Effect of temperature shock on soybean microspore embryogenesis
In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC. Cytological analysis done during the 30 days of culture showed that frequencies...
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Published in: | Brazilian archives of biology and technology Vol. 47; no. 4; pp. 537 - 544 |
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Main Authors: | , , , |
Format: | Journal Article |
Language: | English |
Published: |
Tecpar
01-08-2004
Instituto de Tecnologia do Paraná (Tecpar) |
Subjects: | |
Online Access: | Get full text |
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Summary: | In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC. Cytological analysis done during the 30 days of culture showed that frequencies of symmetrical binucleated and multicellular pollen grain did not differ among temperature treatments. Multicellular pollen grain could be formed by symmetrical division, as well as by assymetrical division. In relation to the embryo induction, the results of the treatments did not differ too. These results suggested that these treatments did not induce a sporophytic pathway in soybean.
Neste estudo foram testados os efeitos do choque térmico na indução androgenética em cultura de anteras de soja. Parte das anteras formam submetidas ao choque térmico de 4 ou 33ºC, enquanto as anteras controle formam mantidas a 25ºC. Análises citológicas formam realizadas ao longo dos 30 primeiros dias de cultura mostrando que a freqüência de grãos de pólen binucleados simétricos e multinucleados não difere entre os tratamentos e que os grãos de pólen multinucleados podem ser formados tanto a partir de uma mitose simétrica inicial como após uma mitose assimétrica. Em relação à indução embriogênica, os tratamentos também não diferem. Estes resultados sugerem que o tratamento de choque térmico não induz a rota esporofítica nos micrósporos de soja. |
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Bibliography: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400006 10.1590/S1516-89132004000400006 |
ISSN: | 1516-8913 1516-8913 1678-4324 |
DOI: | 10.1590/s1516-89132004000400006 |