Resveratrol shortens the chronological lifespan of Saccharomyces cerevisiae by a pro‐oxidant mechanism

Resveratrol shortens the chronological lifespan of Saccharomyces cerevisiae by a pro‐oxidant mechanism

The antioxidant phenotype caused by resveratrol has been recognized as a key piece in the health benefits exerted by this phytochemical in diseases related to aging. It has recently been proposed that a mitochondrial pro‐oxidant mechanism could be the cause of resveratrol antioxidant properties. In...

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Published in:Yeast (Chichester, England) Vol. 39; no. 3; pp. 193 - 207
Main Authors: Canedo‐Santos, Juan Carlos, Carrillo‐Garmendia, Andres, Mora‐Martinez, Iridian, Gutierrez‐Garcia, Ingrid Karina, Ramirez‐Romero, Maria Guadalupe, Regalado‐Gonzalez, Carlos, Nava, Gerardo M., Madrigal‐Perez, Luis Alberto
Format: Journal Article
Language:English
Published: England Wiley Subscription Services, Inc 01-03-2022
Subjects:
Aging
antioxidant systems
Antioxidants
Catalase
Electron transport chain
Glutathione
Life span
Mitochondria
Oxidants
Oxidation
Oxidative stress
Phenotypes
Reactive oxygen species
Resveratrol
Saccharomyces cerevisiae
Semiquinones
Supplements
antioxidant systems
aging
Saccharomyces cerevisiae
resveratrol
catalase
oxidative stress
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Summary:The antioxidant phenotype caused by resveratrol has been recognized as a key piece in the health benefits exerted by this phytochemical in diseases related to aging. It has recently been proposed that a mitochondrial pro‐oxidant mechanism could be the cause of resveratrol antioxidant properties. In this regard, the hypothesis that resveratrol impedes electron transport to complex III of the electron transport chain as its main target suggests that resveratrol could increase reactive oxygen species (ROS) generation through reverse electron transport or by the semiquinones formation. This idea also explains that cells respond to resveratrol oxidative damage, inducing their antioxidant systems. Moreover, resveratrol pro‐oxidant properties could accelerate the aging process, according to the free radical theory of aging, which postulates that organism's age due to the accumulation of the harmful effects of ROS in cells. Nonetheless, there is no evidence linking the chronological lifespan (CLS) shorten occasioned by resveratrol with a pro‐oxidant mechanism. Hence, this study aimed to evaluate whether resveratrol shortens the CLS of Saccharomyces cerevisiae due to a pro‐oxidant activity. Herein, we provide evidence that supplementation with 100 μM of resveratrol at 5% glucose: (1) shortened the CLS of ctt1Δ and yap1Δ strains; (2) decreased ROS levels and increased the catalase activity in WT strain; (3) maintained unaffected the ROS levels and did not change the catalase activity in ctt1Δ strain; and (4) lessened the exponential growth of ctt1Δ strain, which was restored with the adding of reduced glutathione. These results indicate that resveratrol decreases CLS by a pro‐oxidant mechanism. In this article, the authors present evidence of how resveratrol decreases the chronological aging of Saccharomyces cerevisiae through a pro‐oxidant mechanism. Also, they provide insights into the essential role of the cytosolic catalase T to counteract the resveratrol pro‐oxidant phenotype. Take Away High‐glucose supplementation promotes pro‐oxidant resveratrol phenotype. Resveratrol augments chronological aging by a pro‐oxidant mechanism. Catalase T is essential to counteract resveratrol pro‐oxidative properties.
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ISSN:0749-503X
1097-0061
DOI:10.1002/yea.3677