Search Results - "Moore, Ronald J"

Nanodroplet processing platform for deep and quantitative proteome profiling of 10–100 mammalian cells by Zhu, Ying, Piehowski, Paul D., Zhao, Rui, Chen, Jing, Shen, Yufeng, Moore, Ronald J., Shukla, Anil K., Petyuk, Vladislav A., Campbell-Thompson, Martha, Mathews, Clayton E., Smith, Richard D., Qian, Wei-Jun, Kelly, Ryan T.

“…Nanoscale or single-cell technologies are critical for biomedical applications. However, current mass spectrometry (MS)-based proteomic approaches require…”
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Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC‐MS by Zhu, Ying, Clair, Geremy, Chrisler, William B., Shen, Yufeng, Zhao, Rui, Shukla, Anil K., Moore, Ronald J., Misra, Ravi S., Pryhuber, Gloria S., Smith, Richard D., Ansong, Charles, Kelly, Ryan T.

“…We report on the quantitative proteomic analysis of single mammalian cells. Fluorescence‐activated cell sorting was employed to deposit cells into a newly…”
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Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution by Piehowski, Paul D., Zhu, Ying, Bramer, Lisa M., Stratton, Kelly G., Zhao, Rui, Orton, Daniel J., Moore, Ronald J., Yuan, Jia, Mitchell, Hugh D., Gao, Yuqian, Webb-Robertson, Bobbie-Jo M., Dey, Sudhansu K., Kelly, Ryan T., Burnum-Johnson, Kristin E.

“…Biological tissues exhibit complex spatial heterogeneity that directs the functions of multicellular organisms. Quantifying protein expression is essential for…”
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High-Throughput Single Cell Proteomics Enabled by Multiplex Isobaric Labeling in a Nanodroplet Sample Preparation Platform by Dou, Maowei, Clair, Geremy, Tsai, Chia-Feng, Xu, Kerui, Chrisler, William B, Sontag, Ryan L, Zhao, Rui, Moore, Ronald J, Liu, Tao, Pasa-Tolic, Ljiljana, Smith, Richard D, Shi, Tujin, Adkins, Joshua N, Qian, Wei-Jun, Kelly, Ryan T, Ansong, Charles, Zhu, Ying

“…Effective extension of mass spectrometry-based proteomics to single cells remains challenging. Herein we combined microfluidic nanodroplet technology with…”
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High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip by Woo, Jongmin, Williams, Sarah M., Markillie, Lye Meng, Feng, Song, Tsai, Chia-Feng, Aguilera-Vazquez, Victor, Sontag, Ryan L., Moore, Ronald J., Hu, Dehong, Mehta, Hardeep S., Cantlon-Bruce, Joshua, Liu, Tao, Adkins, Joshua N., Smith, Richard D., Clair, Geremy C., Pasa-Tolic, Ljiljana, Zhu, Ying

“…Global quantification of protein abundances in single cells could provide direct information on cellular phenotypes and complement transcriptomics…”
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Dengue virus infection perturbs lipid homeostasis in infected mosquito cells by Perera, Rushika, Riley, Catherine, Isaac, Giorgis, Hopf-Jannasch, Amber S, Moore, Ronald J, Weitz, Karl W, Pasa-Tolic, Ljiljana, Metz, Thomas O, Adamec, Jiri, Kuhn, Richard J

“…Dengue virus causes ∼50-100 million infections per year and thus is considered one of the most aggressive arthropod-borne human pathogen worldwide. During its…”
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Active molecular iodine photochemistry in the Arctic by Raso, Angela R. W., Custard, Kyle D., May, Nathaniel W., Tanner, David, Newburn, Matt K., Walker, Lawrence, Moore, Ronald J., Huey, L. G., Alexander, Liz, Shepson, Paul B., Pratt, Kerri A.

“…During springtime, the Arctic atmospheric boundary layer undergoes frequent rapid depletions in ozone and gaseous elemental mercury due to reactions with…”
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Subnanogram proteomics: Impact of LC column selection, MS instrumentation and data analysis strategy on proteome coverage for trace samples by Zhu, Ying, Zhao, Rui, Piehowski, Paul D., Moore, Ronald J., Lim, Sujung, Orphan, Victoria J., Paša-Tolić, Ljiljana, Qian, Wei-Jun, Smith, Richard D., Kelly, Ryan T.

“…[Display omitted] •In-depth proteome analysis was achieved for low- and subnanogram biological samples.•NanoLC with 30-μm-i.d. column and Orbitrap Fusion Lumos…”
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Effectiveness of CID, HCD, and ETD with FT MS/MS for Degradomic-Peptidomic Analysis: Comparison of Peptide Identification Methods by Shen, Yufeng, Tolić, Nikola, Xie, Fang, Zhao, Rui, Purvine, Samuel O, Schepmoes, Athena A, Moore, Ronald, J, Anderson, Gordon A, Smith, Richard D

“…We report on the effectiveness of CID, HCD, and ETD for LC–FT MS/MS analysis of peptides using a tandem linear ion trap-Orbitrap mass spectrometer. A range of…”
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Antibody-free, targeted mass-spectrometric approach for quantification of proteins at low picogram per milliliter levels in human plasma/serum by Shi, Tujin, Fillmore, Thomas L, Sun, Xuefei, Zhao, Rui, Schepmoes, Athena A, Hossain, Mahmud, Xie, Fang, Wu, Si, Kim, Jong-Seo, Jones, Nathan, Moore, Ronald J, Paša-Tolić, Ljiljana, Kagan, Jacob, Rodland, Karin D, Liu, Tao, Tang, Keqi, Camp, David G, Smith, Richard D, Qian, Wei-Jun

“…Sensitive detection of low-abundance proteins in complex biological samples has typically been achieved by immunoassays that use antibodies specific to target…”
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Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples by Dou, Maowei, Tsai, Chia-Feng, Piehowski, Paul D, Wang, Yang, Fillmore, Thomas L, Zhao, Rui, Moore, Ronald J, Zhang, Pengfei, Qian, Wei-Jun, Smith, Richard D, Liu, Tao, Kelly, Ryan T, Shi, Tujin, Zhu, Ying

“…Two-dimensional reversed-phase capillary liquid chromatography (2D RPLC) separations have enabled comprehensive proteome profiling of biological systems…”
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Chemically Etched Open Tubular and Monolithic Emitters for Nanoelectrospray Ionization Mass Spectrometry by Kelly, Ryan T, Page, Jason S, Luo, Quanzhou, Moore, Ronald J, Orton, Daniel J, Tang, Keqi, Smith, Richard D

“…We have developed a new procedure for fabricating fused-silica emitters for electrospray ionization-mass spectrometry (ESI-MS) in which the end of a bare…”
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Enhanced top-down characterization of histone post-translational modifications by Tian, Zhixin, Tolić, Nikola, Zhao, Rui, Moore, Ronald J, Hengel, Shawna M, Robinson, Errol W, Stenoien, David L, Wu, Si, Smith, Richard D, Paša-Tolić, Ljiljana

“…Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone…”
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Spatially-Resolved Proteomics: Rapid Quantitative Analysis of Laser Capture Microdissected Alveolar Tissue Samples by Clair, Geremy, Piehowski, Paul D., Nicola, Teodora, Kitzmiller, Joseph A., Huang, Eric L., Zink, Erika M., Sontag, Ryan L., Orton, Daniel J., Moore, Ronald J., Carson, James P., Smith, Richard D., Whitsett, Jeffrey A., Corley, Richard A., Ambalavanan, Namasivayam, Ansong, Charles

“…Laser capture microdissection (LCM)-enabled region-specific tissue analyses are critical to better understand complex multicellular processes. However, current…”
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Stochiometric quantification of the thiol redox proteome of macrophages reveals subcellular compartmentalization and susceptibility to oxidative perturbations by Duan, Jicheng, Zhang, Tong, Gaffrey, Matthew J., Weitz, Karl K., Moore, Ronald J., Li, Xiaolu, Xian, Ming, Thrall, Brian D., Qian, Wei-Jun

“…Posttranslational modifications of protein cysteine thiols play a significant role in redox regulation and the pathogenesis of human diseases. Herein, we…”
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Proteome-wide Light/Dark Modulation of Thiol Oxidation in Cyanobacteria Revealed by Quantitative Site-specific Redox Proteomics by Guo, Jia, Nguyen, Amelia Y., Dai, Ziyu, Su, Dian, Gaffrey, Matthew J., Moore, Ronald J., Jacobs, Jon M., Monroe, Matthew E., Smith, Richard D., Koppenaal, David W., Pakrasi, Himadri B., Qian, Wei-Jun

“…Reversible protein thiol oxidation is an essential regulatory mechanism of photosynthesis, metabolism, and gene expression in photosynthetic organisms. Herein,…”
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Mass spectrometry-based direct detection of multiple types of protein thiol modifications in pancreatic beta cells under endoplasmic reticulum stress by Li, Xiaolu, Day, Nicholas J., Feng, Song, Gaffrey, Matthew J., Lin, Tai-Du, Paurus, Vanessa L., Monroe, Matthew E., Moore, Ronald J., Yang, Bin, Xian, Ming, Qian, Wei-Jun

“…Thiol-based post-translational modifications (PTMs) play a key role in redox-dependent regulation and signaling. Functional cysteine (Cys) sites serve as redox…”
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A reversed-phase capillary ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) method for comprehensive top-down/bottom-up lipid profiling by Gao, Xiaoli, Zhang, Qibin, Meng, Da, Isaac, Giorgis, Zhao, Rui, Fillmore, Thomas L., Chu, Rosey K., Zhou, Jianying, Tang, Keqi, Hu, Zeping, Moore, Ronald J., Smith, Richard D., Katze, Michael G., Metz, Thomas O.

“…Lipidomics is a critical part of metabolomics and aims to study all the lipids within a living system. We present here the development and evaluation of a…”
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Characterization of intact N- and O-linked glycopeptides using higher energy collisional dissociation by Cao, Li, Tolić, Nikola, Qu, Yi, Meng, Da, Zhao, Rui, Zhang, Qibin, Moore, Ronald J., Zink, Erika M., Lipton, Mary S., Paša-Tolić, Ljiljana, Wu, Si

“…Simultaneous elucidation of the glycan structure and the glycosylation site are needed to reveal the biological function of protein glycosylation. In this…”
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SNaPP: Simplified Nanoproteomics Platform for Reproducible Global Proteomic Analysis of Nanogram Protein Quantities by Huang, Eric L, Piehowski, Paul D, Orton, Daniel J, Moore, Ronald J, Qian, Wei-Jun, Casey, Cameron P, Sun, Xiaofei, Dey, Sudhansu K, Burnum-Johnson, Kristin E, Smith, Richard D

“…Global proteomic analyses of complex protein samples in nanogram quantities require a fastidious approach to achieve in-depth protein coverage and quantitative…”
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