Relationships between natural T cells, atopy, IgE levels, and IL‐4 production

Relationships between natural T cells, atopy, IgE levels, and IL‐4 production

Background: Th2 cells govern allergic disorders. Mechanisms leading to the Th2 commitment are dominated by the requirement of IL‐4. A potential source of this triggering IL‐4 could be the CD4+ subset of a small population of T cells, natural T (NT) cells. Indeed, this subset is involved in IgE respo...

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Bibliographic Details
Published in:Allergy (Copenhagen) Vol. 55; no. 3; pp. 286 - 290
Main Authors: Magnan, A., Mély, L., Prato, S., Vervloet, D., Romagné, F., Camilla, C., Necker, A., Casano, B., Montero‐Jullian, F., Malissen, B., Bongrand, P., Fert, V.
Format: Journal Article
Language:English
Published: Copenhagen Munksgaard International Publishers 01-03-2000
Blackwell
Subjects:
Adolescent
Adult
AIDS/HIV
Allergic diseases
allergy
atopy
Biological and medical sciences
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Cell Culture Techniques
Flow Cytometry
General aspects
Humans
Hypersensitivity - immunology
IgE
IL‐4
Immunoglobulin E - analysis
Immunopathology
Immunophenotyping
Interleukin-4 - biosynthesis
Intradermal Tests
Lymphocyte Count
Medical sciences
Middle Aged
NT cells
T cells
Th2
Th2 Cells - immunology
Human
Allergy
Immunopathology
Immunoglobulins
IgE
Cytokine
Helper cell
Natural
Cell subpopulation
Atopy
Interleukin 4
T-Lymphocyte
Th2 lymphocyte
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Summary:Background: Th2 cells govern allergic disorders. Mechanisms leading to the Th2 commitment are dominated by the requirement of IL‐4. A potential source of this triggering IL‐4 could be the CD4+ subset of a small population of T cells, natural T (NT) cells. Indeed, this subset is involved in IgE responses in mice and produces promptly high amounts of IL‐4 in both mice and man. Methods: NT cells were identified in peripheral blood by flow cytometry with antibodies against Vα24 and Vβ11, recognizing the T‐cell receptor specific for NT cells. Simultaneous staining with anti‐CD3, anti‐CD4, or anti‐CD8 antibodies was performed. The frequency of NT cells in man was studied according to the presence of atopy defined by the positivity of skin tests, according to total IgE levels in serum, and according to IL‐4 concentration of whole‐blood culture supernatants determined by a flow cytometer microsphere‐based assay. Results: Seventy subjects were included, of whom 30 were atopic. The number of CD4+ NT cells was higher in atopics than in nonatopics (P=0.009). This number was correlated to the total IgE levels (r=0.34, P=0.03). In addition, the number of CD4+ NT cells, but also of CD8+ NT cells, was correlated to the levels of IL‐4 (r=0.71, P=0.01, and r=0.6, P=0.03, respectively). Conclusions: These results show that the number of NT cells, particularly the CD4+ subset, is related to atopy, IL‐4 production, and IgE levels. Therefore, this population of T cells is likely to play a role in the Th2 commitment initiating atopic diseases.
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ISSN:0105-4538
1398-9995
DOI:10.1034/j.1398-9995.2000.00425.x