Baccharis trimera (Less.) DC Exhibits an Anti-Adipogenic Effect by Inhibiting the Expression of Proteins Involved in Adipocyte Differentiation

(Less.) DC (gorse) is a plant popularly used for the treatment of obesity. In this study, we prepared three extracts aqueous extract (AE), decoction (AE-D), and methanol extract (ME) and investigated their antioxidant effects in six different tests and their anti-adipogenic effect in 3T3-L1 cells. T...

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Bibliographic Details
Published in:	Molecules (Basel, Switzerland) Vol. 22; no. 6; p. 972
Main Authors:	de Souza Marinho do Nascimento, Daniele, Oliveira, Ruth Medeiros, Camara, Rafael Barros Gomes, Gomes, Dayanne Lopes, Monte, Jessika Fernanda Santiago, Costa, Mariana Santana Santos Pereira, Fernandes, Júlia Moraes, Langassner, Silvana Maria Zucolotto, Rocha, Hugo Alexandre Oliveira
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 12-06-2017 MDPI
Subjects:	3T3-L1 Cells Adipocytes Adipocytes - drug effects Adipocytes - metabolism Adipogenesis Adipogenesis - drug effects Animals antioxidant Antioxidants Antioxidants - chemistry Assaying Baccharis - chemistry CCAAT-Enhancer-Binding Protein-beta - genetics CCAAT-Enhancer-Binding Proteins - genetics CCAAT/enhancer-binding protein Cell Differentiation - drug effects Chelation chlorogenic acid Cytotoxicity Ferric ions Functional foods & nutraceuticals Gene Expression Regulation - drug effects Hydroxyl radicals Mice Obesity - drug therapy Obesity - genetics Obesity - pathology Plant Extracts - administration & dosage Plant Extracts - chemistry PPAR gamma - genetics Proteins Superoxide Transcription factors antioxidant chlorogenic acid adipogenesis
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Summary:	(Less.) DC (gorse) is a plant popularly used for the treatment of obesity. In this study, we prepared three extracts aqueous extract (AE), decoction (AE-D), and methanol extract (ME) and investigated their antioxidant effects in six different tests and their anti-adipogenic effect in 3T3-L1 cells. The extracts showed a dose-dependent antioxidant activity in all tests. AE was the most potent antioxidant in copper and ferric ion chelation assays, whereas AE-D was the most potent in superoxide and hydroxyl radical scavenging assays, reducing power assay, and total antioxidant capacity analysis. Only ME showed a cytotoxic effect against 3T3-L1 cells. Lipid accumulation decreased in 3T3-L1 adipocytes in the presence of AE and AE-D extracts (0.5 to 1.0 mg/mL). In addition, the extracts dramatically attenuated the levels of adipogenic transcriptional factors, including CCAAT enhancer-binding protein α (C/EBPα), CCAAT enhancer-binding protein β (C/EBPβ), and gamma receptors by peroxisome proliferators (PPARγ), during adipogenesis. AE-D (1.0 mg/mL) caused an approximately 90% reduction in the levels of these molecules. We propose that has an anti-adipogenic effect and could be used in the development of functional foods.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1420-3049 1420-3049
DOI:	10.3390/molecules22060972