Epicatechin-3-O-(3″-O-methyl)-gallate Content in Various Tea Cultivars (Camellia sinensis L.) and Its in Vitro Inhibitory Effect on Histamine Release

It has been reported that epigallocatechin-3-O-(3″-O-methyl)-gallate (EGCG3″Me) and the EGCG3″Me-rich green tea (Camellia sinensis L.) cultivar ‘Benifuuki’ exhibit antiallergic effects. The objective of this study was to investigate the effect of various tea leaf catechins on histamine release from...

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Bibliographic Details
Published in:	Journal of agricultural and food chemistry Vol. 60; no. 9; pp. 2165 - 2170
Main Authors:	Maeda-Yamamoto, Mari, Ema, Kaori, Monobe, Mamami, Tokuda, Yoshiko, Tachibana, Hirofumi
Format:	Journal Article
Language:	English
Published:	Washington, DC American Chemical Society 07-03-2012
Subjects:	Animals Biological and medical sciences bone marrow Bone Marrow Cells - metabolism Camellia sinensis Camellia sinensis - chemistry Catechin - analogs & derivatives Catechin - pharmacology Coffee, tea and other stimulative beverage industries correlation cultivars epigallocatechin Female flavanols Food industries Fundamental and applied biological sciences. Psychology Gallic Acid - analogs & derivatives Gallic Acid - analysis Gallic Acid - pharmacology green tea high performance liquid chromatography histamine Histamine Release - drug effects Hypersensitivity - drug therapy leaves mast cells Mast Cells - metabolism Mice Plant Leaves - chemistry Species Specificity epicatechin-3-O-(3″-O-methyl)-gallate (ECG3″Me) HPLC analysis ‘Benifuuki’ tea histamine release inhibitory effect Flavanol 'Benifuuki' tea In vitro Tea Camellia sinensis Dicotyledones Angiospermae Spermatophyta epitatechin-3-O-(3-O-methyl)-gallate (ECG3Me) Theaceae Cultivar
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Summary:	It has been reported that epigallocatechin-3-O-(3″-O-methyl)-gallate (EGCG3″Me) and the EGCG3″Me-rich green tea (Camellia sinensis L.) cultivar ‘Benifuuki’ exhibit antiallergic effects. The objective of this study was to investigate the effect of various tea leaf catechins on histamine release from murine bone marrow mast cells (BMMC). At a dose of 50 μg/mL, the rank order of histamine release inhibition was observed to be epicatechin-3-O-(3″-O-methyl)-gallate (ECG3″Me) > gallocatechin-3-O-(3″-O-methyl)-gallate (GCG3″Me) > EGCG3″Me > gallocatechin-gallate (GCG) > catechin-gallate (CG) > EGCG > epicatechin-gallate (ECG) > epigallocatechin (EGC) > gallocatechin (GC). Of the various tea cultivars analyzed by HPLC, the greatest content of ECG3″Me was found in the third crop of ‘Benifuuki’ (1.05% dry weight). Moreover, ECG3″Me content was positively correlated with EGCG3″Me content in ‘Benifuuki’ tea leaves. In an assay of mixtures of ECG3″Me and EGCG3″Me, inhibitory activity (50 μg/mL in total) was increased as the content of ECG3″Me increased. This suggests that ECG3″Me might link to the antiallergic effect of ‘Benifuuki’ tea, as has been reported for EGCG3″Me.
Bibliography:	http://dx.doi.org/10.1021/jf204497b ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-8561 1520-5118
DOI:	10.1021/jf204497b