An immunosorbent for interferon purification

Immunoaffinity chromatography was shown to be the method achieving the most complete elimination of antigenic admixtures from leukocyte interferon preparations without the loss of the preparation activity. An affinity sorbent has been developed on the basis of covalently linked polyvinyl alcohol (PV...

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Bibliographic Details
Published in:	Voprosy virusologiĭ Vol. 38; no. 3; p. 129
Main Authors:	Garipova, M I, Monakov, Iu B, Baschenko, I A, Frolova, I S, Kleeva, O B, Kuznetsov, V P
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-05-1993
Subjects:	Animals Antigens - isolation & purification Cells, Cultured Chromatography, Affinity - methods Drug Contamination Humans Immunosorbent Techniques Immunosorbents Interferon Inducers - pharmacology Interferons - isolation & purification Leukocytes - drug effects Ovalbumin - isolation & purification Polyvinyl Alcohol Rabbits
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Summary:	Immunoaffinity chromatography was shown to be the method achieving the most complete elimination of antigenic admixtures from leukocyte interferon preparations without the loss of the preparation activity. An affinity sorbent has been developed on the basis of covalently linked polyvinyl alcohol (PVA). The immobilization of the antigen-specific rabbit globulin in the preparation of the sorbent is achieved by reaction of protein amino groups with the activated matrix. The proposed sorbent achieved the elimination of the antigenic admixtures from interferon preparations as effectively as those prepared on the basis of sepharose 4B, the productivity of the purification process being at least 5 times higher. The proposed sorbent is stable at the limit values of rH, is not destroyed by detergents, is sterilized in the process of preparation. Owing to the strong linkage of the immobilized immunoglobulin with the PVA-carrier, immunoaffinity chromatography on this sorbent does not involve contamination of the preparations with rabbit globulin allergenic for man. The combination of a large pore structure, wetting ability, stiffness, mechanical and chemical stability allows the proposed sorbent to be recommended for use in modern large-scale biotechnological production.
ISSN:	0507-4088