Search Results - "Moller, Jesper V."

Inhibition of the sarco/endoplasmic reticulum (ER) Ca2+-ATPase by thapsigargin analogs induces cell death via ER Ca2+ depletion and the unfolded protein response by Sehgal, Pankaj, Szalai, Paula, Olesen, Claus, Praetorius, Helle A., Nissen, Poul, Christensen, Søren Brøgger, Engedal, Nikolai, Møller, Jesper V.

“…Calcium (Ca2+) is a fundamental regulator of cell signaling and function. Thapsigargin (Tg) blocks the sarco/endoplasmic reticulum (ER) Ca2+-ATPase (SERCA),…”
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The sarcolipin-bound calcium pump stabilizes calcium sites exposed to the cytoplasm by Winther, Anne-Marie L., Bublitz, Maike, Karlsen, Jesper L., Møller, Jesper V., Hansen, John B., Nissen, Poul, Buch-Pedersen, Morten J.

“…An X-ray crystal structure of sarcoplasmic reticulum Ca 2+ -ATPase (SERCA) in the presence of sarcolipin, a SERCA regulator, is presented; the structure shows…”
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Comparing crystal structures of Ca²⁺‐ATPase in the presence of different lipids by Drachmann, Nikolaj D, Olesen, Claus, Møller, Jesper V, Guo, Zheng, Nissen, Poul, Bublitz, Maike

“…The activity of the sarco/endoplasmic reticulum Ca²⁺‐ATPase (SERCA) depends strongly on the lipid composition of the surrounding membrane. Yet, structural…”
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ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay by Sehgal, Pankaj, Olesen, Claus, Møller, Jesper V

“…Enzymatic coupled assays are usually based on the spectrophotometric registration of changes in NADH/NAD(+) or NADPH/NADP(+) absorption at 340 nm accompanying…”
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Engineering a prostate-specific membrane antigen-activated tumor endothelial cell prodrug for cancer therapy by Denmeade, Samuel R, Mhaka, Annastasiah M, Rosen, D Marc, Brennen, W Nathaniel, Dalrymple, Susan, Dach, Ingrid, Olesen, Claus, Gurel, Bora, Demarzo, Angelo M, Wilding, George, Carducci, Michael A, Dionne, Craig A, Møller, Jesper V, Nissen, Poul, Christensen, S Brøgger, Isaacs, John T

“…Heterogeneous expression of drug target proteins within tumor sites is a major mechanism of resistance to anticancer therapies. We describe a strategy to…”
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The SERCA residue Glu340 mediates interdomain communication that guides Ca2+ transport by Geurts, Maxwell M. G., Clausen, Johannes D., Arnou, Bertrand, Montigny, Cédric, Lenoir, Guillaume, Corey, Robin A., Jaxel, Christine, Møller, Jesper V., Nissen, Poul, Andersen, Jens Peter, le Maire, Marc, Bublitz, Maike

“…The sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) is a P-type ATPase that transports Ca2+ from the cytosol into the sarco(endo) plasmic reticulum (SR/ER)…”
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Crystallographic analysis reveals a unique lidocaine binding site on human serum albumin by Hein, Kim Langmach, Kragh-Hansen, Ulrich, Morth, J. Preben, Jeppesen, Martin D., Otzen, Daniel, Møller, Jesper V., Nissen, Poul

“…Human serum albumin (HSA), the major protein component in blood plasma and in extravascular spaces, is known to participate in the binding and transport of a…”
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Invisible detergents for structure determination of membrane proteins by small‐angle neutron scattering by Midtgaard, Søren Roi, Darwish, Tamim A., Pedersen, Martin Cramer, Huda, Pie, Larsen, Andreas Haahr, Jensen, Grethe Vestergaard, Kynde, Søren Andreas Røssell, Skar‐Gislinge, Nicholas, Nielsen, Agnieszka Janina Zygadlo, Olesen, Claus, Blaise, Mickael, Dorosz, Jerzy Józef, Thorsen, Thor Seneca, Venskutonytė, Raminta, Krintel, Christian, Møller, Jesper V., Frielinghaus, Henrich, Gilbert, Elliot Paul, Martel, Anne, Kastrup, Jette Sandholm, Jensen, Poul Erik, Nissen, Poul, Arleth, Lise

“…A novel and generally applicable method for determining structures of membrane proteins in solution via small‐angle neutron scattering (SANS) is presented…”
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Slow Phospholipid Exchange between a Detergent-Solubilized Membrane Protein and Lipid-Detergent Mixed Micelles: Brominated Phospholipids as Tools to Follow Its Kinetics by Montigny, Cédric, Dieudonné, Thibaud, Orlowski, Stéphane, Vázquez-Ibar, José Luis, Gauron, Carole, Georgin, Dominique, Lund, Sten, le Maire, Marc, Møller, Jesper V, Champeil, Philippe, Lenoir, Guillaume

“…Membrane proteins are largely dependent for their function on the phospholipids present in their immediate environment, and when they are solubilized by…”
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Differential effects of thapsigargin analogues on apoptosis of prostate cancer cells: Complex regulation by intracellular calcium by Dubois, Charlotte, Vanden Abeele, Fabien, Sehgal, Pankaj, Olesen, Claus, Junker, Steffen, Christensen, Søren B, Prevarskaya, Natalia, Møller, Jesper V

“…The inhibition of sarcoplasmic reticulum Ca²⁺‐ATPase (SERCA) by thapsigargin (Tg) and Tg‐type analogues is considered to trigger cell death by activation of…”
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Critical Roles of Hydrophobicity and Orientation of Side Chains for Inactivation of Sarcoplasmic Reticulum Ca2+-ATPase with Thapsigargin and Thapsigargin Analogs by Winther, Anne-Marie L., Liu, Huizhen, Sonntag, Yonathan, Olesen, Claus, le Maire, Marc, Soehoel, Helmer, Olsen, Carl-Erik, Christensen, S. Brøgger, Nissen, Poul, Møller, Jesper V.

“…Thapsigargin (Tg), a specific inhibitor of sarco/endoplasmic Ca2+-ATPases (SERCA), binds with high affinity to the E2 conformation of these ATPases. SERCA…”
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Purified E255L Mutant SERCA1a and Purified PfATP6 Are Sensitive to SERCA-type Inhibitors but Insensitive to Artemisinins by Cardi, Delphine, Pozza, Alexandre, Arnou, Bertrand, Marchal, Estelle, Clausen, Johannes D., Andersen, Jens Peter, Krishna, Sanjeev, Møller, Jesper V., le Maire, Marc, Jaxel, Christine

“…The antimalarial drugs artemisinins have been described as inhibiting Ca2+-ATPase activity of PfATP6 (Plasmodium falciparum ATP6) after expression in Xenopus…”
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Gel chromatography and analytical ultracentrifugation to determine the extent of detergent binding and aggregation, and Stokes radius of membrane proteins using sarcoplasmic reticulum Ca2+–ATPase as an example by le Maire, Marc, Arnou, Bertrand, Olesen, Claus, Georgin, Dominique, Ebel, Christine, Møller, Jesper V

“…For structural studies of integral membrane proteins, including their 3D crystallization, the judicious use of detergent for solubilization and purification is…”
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Water-Mediated Interactions Influence the Binding of Thapsigargin to Sarco/Endoplasmic Reticulum Calcium Adenosinetriphosphatase by Paulsen, Eleonora S, Villadsen, Jesper, Tenori, Eleonora, Liu, Huizhen, Bonde, Ditte F, Lie, Mette A, Bublitz, Maike, Olesen, Claus, Autzen, Henriette E, Dach, Ingrid, Sehgal, Pankaj, Nissen, Poul, Møller, Jesper V, Schiøtt, Birgit, Christensen, S. Brøgger

“…A crystal structure suggests four water molecules are present in the binding cavity of thapsigargin in sarco/endoplasmic reticulum calcium ATPase (SERCA)…”
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Discovery of Tricyclic Clerodane Diterpenes as Sarco/Endoplasmic Reticulum Ca2+-ATPase Inhibitors and Structure–Activity Relationships by De Ford, Christian, Calderón, Carlos, Sehgal, Pankaj, Fedosova, Natalya U, Murillo, Renato, Olesen, Claus, Nissen, Poul, Møller, Jesper V, Merfort, Irmgard

“…Tricyclic clerodane diterpenes (TCDs) are natural compounds that often show potent cytotoxicity for cancer cells, but their mode of action remains elusive. A…”
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Active Detergent-solubilized H+,K+-ATPase Is a Monomer by Dach, Ingrid, Olesen, Claus, Signor, Luca, Nissen, Poul, le Maire, Marc, Møller, Jesper V., Ebel, Christine

“…The H+,K+-ATPase pumps protons or hydronium ions and is responsible for the acidification of the gastric fluid. It is made up of an α-catalytic and a…”
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Structural studies of P-type ATPase-ligand complexes using an X-ray free-electron laser by Bublitz, Maike, Nass, Karol, Drachmann, Nikolaj D, Markvardsen, Anders J, Gutmann, Matthias J, Barends, Thomas R M, Mattle, Daniel, Shoeman, Robert L, Doak, R Bruce, Boutet, Sébastien, Messerschmidt, Marc, Seibert, Marvin M, Williams, Garth J, Foucar, Lutz, Reinhard, Linda, Sitsel, Oleg, Gregersen, Jonas L, Clausen, Johannes D, Boesen, Thomas, Gotfryd, Kamil, Wang, Kai-Tuo, Olesen, Claus, Møller, Jesper V, Nissen, Poul, Schlichting, Ilme

“…Membrane proteins are key players in biological systems, mediating signalling events and the specific transport of e.g. ions and metabolites. Consequently,…”
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P-type ATPases as drug targets: Tools for medicine and science by Yatime, Laure, Buch-Pedersen, Morten J., Musgaard, Maria, Morth, J. Preben, Winther, Anne-Marie Lund, Pedersen, Bjørn P., Olesen, Claus, Andersen, Jens Peter, Vilsen, Bente, Schiøtt, Birgit, Palmgren, Michael G., Møller, Jesper V., Nissen, Poul, Fedosova, Natalya

“…P-type ATPases catalyze the selective active transport of ions like H +, Na +, K +, Ca 2+, Zn 2+, and Cu 2+ across diverse biological membrane systems. Many…”
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Protein–protein contacts in solubilized membrane proteins, as detected by cross-linking by le Maire, Marc, Møller, Jesper V., Menguy, Thierry, Velours, Jean, Champeil, Philippe

“…The amount of detergent required for the solubilization of membrane proteins needs to be optimised as an excess may cause loss of activity and insufficiency…”
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Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop by Champeil, Philippe, de Foresta, Béatrice, Picard, Martin, Gauron, Carole, Georgin, Dominique, le Maire, Marc, Møller, Jesper V, Lenoir, Guillaume, Montigny, Cédric

“…The present study mainly consists of a re-evaluation of the rate at which C12E8, a typical non-ionic detergent used for membrane studies, is able to dissociate…”
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