Search Results - "Molhoj, Michael"

Identification of potential sites for tryptophan oxidation in recombinant antibodies using tert-butylhydroperoxide and quantitative LC-MS by Hensel, Miriam, Steurer, Rebecca, Fichtl, Juergen, Elger, Carsten, Wedekind, Frank, Petzold, Andreas, Schlothauer, Tilman, Molhoj, Michael, Reusch, Dietmar, Bulau, Patrick

“…Amino acid oxidation is known to affect the structure, activity, and rate of degradation of proteins. Methionine oxidation is one of the several chemical…”
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From proof of concept to the routine use of an automated and robust multi-dimensional liquid chromatography mass spectrometry workflow applied for the charge variant characterization of therapeutic antibodies by Goyon, Alexandre, Dai, Lu, Chen, Tao, Wei, Bingchuan, Yang, Feng, Andersen, Nisana, Kopf, Robert, Leiss, Michael, Mølhøj, Michael, Guillarme, Davy, Stella, Cinzia

“…•Thorough optimization of a multi-dimensional LC/MS setup to identify charge variants.•Similar levels of most protein PTMs reported by off-line and on-line…”
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Structure-based prediction of asparagine and aspartate degradation sites in antibody variable regions by Sydow, Jasmin F, Lipsmeier, Florian, Larraillet, Vincent, Hilger, Maximiliane, Mautz, Bjoern, Mølhøj, Michael, Kuentzer, Jan, Klostermann, Stefan, Schoch, Juergen, Voelger, Hans R, Regula, Joerg T, Cramer, Patrick, Papadimitriou, Apollon, Kettenberger, Hubert

“…Monoclonal antibodies (mAbs) and proteins containing antibody domains are the most prevalent class of biotherapeutics in diverse indication areas. Today,…”
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Applying mini-bore HPAEC-MS/MS for the characterization and quantification of Fc N-glycans from heterogeneously glycosylated IgGs by Maier, Maria, Reusch, Dietmar, Bruggink, Cees, Bulau, Patrick, Wuhrer, Manfred, Mølhøj, Michael

“…⿢HPAEC coupled to ion trap MS using a prototype mini-bore (1mm) CarboPac PA200 column.⿢Improved signal-to-noise with protonated ions relative to…”
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Inter-laboratory study to evaluate the performance of automated online characterization of antibody charge variants by multi-dimensional LC-MS/MS by Camperi, Julien, Grunert, Ingrid, Heinrich, Katrin, Winter, Martin, Özipek, Saban, Hoelterhoff, Sina, Weindl, Thomas, Mayr, Kilian, Bulau, Patrick, Meier, Monika, Mølhøj, Michael, Leiss, Michael, Guillarme, Davy, Bathke, Anja, Stella, Cinzia

“…An international study was conducted to evaluate the performance and reliability of an online multi-dimensional (mD)-LC-MS/MS approach for the characterization…”
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Simultaneous assessment of Asp isomerization and Asn deamidation in recombinant antibodies by LC-MS following incubation at elevated temperatures by Diepold, Katharina, Bomans, Katrin, Wiedmann, Michael, Zimmermann, Boris, Petzold, Andreas, Schlothauer, Tilman, Mueller, Robert, Moritz, Bernd, Stracke, Jan Olaf, Mølhøj, Michael, Reusch, Dietmar, Bulau, Patrick

“…The degradation of proteins by asparagine deamidation and aspartate isomerization is one of several chemical degradation pathways for recombinant antibodies…”
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Variable heavy-variable light domain and Fab-arm CrossMabs with charged residue exchanges to enforce correct light chain assembly by Regula, Joerg Thomas, Imhof-Jung, Sabine, Mølhøj, Michael, Benz, Joerg, Ehler, Andreas, Bujotzek, Alexander, Schaefer, Wolfgang, Klein, Christian

“…Abstract Technologies for the production of bispecific antibodies need to overcome two major challenges. The first one is correct heavy chain assembly, which…”
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Assessment of chemical modifications of sites in the CDRs of recombinant antibodies: Susceptibility vs. functionality of critical quality attributes by Haberger, Markus, Bomans, Katrin, Diepold, Katharina, Hook, Michaela, Gassner, Jana, Schlothauer, Tilman, Zwick, Adrian, Spick, Christian, Kepert, Jochen Felix, Hienz, Brigitte, Wiedmann, Michael, Beck, Hermann, Metzger, Philipp, Mølhøj, Michael, Knoblich, Constanze, Grauschopf, Ulla, Reusch, Dietmar, Bulau, Patrick

“…Modifications like asparagine deamidation, aspartate isomerization, methionine oxidation, and lysine glycation are typical degradations for recombinant…”
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A highly stable polyethylene glycol-conjugated human single-chain antibody neutralizing granulocyte-macrophage colony stimulating factor at low nanomolar concentration by Krinner, Eva-Maria, Hepp, Julia, Hoffmann, Patrick, Bruckmaier, Sandra, Petersen, Laetitia, Petsch, Silke, Parr, Larissa, Schuster, Ioana, Mangold, Susanne, Lorenczewski, Grit, Lutterbüse, Petra, Buziol, Stefan, Hochheim, Inessa, Volkland, Jörg, Mølhøj, Michael, Sriskandarajah, Mirnalini, Strasser, Markus, Itin, Christian, Wolf, Andreas, Basu, Amartya, Yang, Karen, Filpula, David, Sørensen, Poul, Kufer, Peter, Baeuerle, Patrick, Raum, Tobias

“…GM-CSF (granulocyte-macrophage colony stimulating factor) plays a central role in inflammatory processes. Treatment with antibodies neutralizing murine GM-CSF…”
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Identification and monitoring of host cell proteins by mass spectrometry combined with high performance immunochemistry testing by Bomans, Katrin, Lang, Antje, Roedl, Veronika, Adolf, Lisa, Kyriosoglou, Kyrillos, Diepold, Katharina, Eberl, Gabriele, Mølhøj, Michael, Strauss, Ulrike, Schmalz, Christian, Vogel, Rudolf, Reusch, Dietmar, Wegele, Harald, Wiedmann, Michael, Bulau, Patrick

“…Biotherapeutics are often produced in non-human host cells like Escherichia coli, yeast, and various mammalian cell lines. A major focus of any therapeutic…”
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Detection of a phosphorylated glycine-serine linker in an IgG-based fusion protein by Tyshchuk, Oksana, Völger, Hans Rainer, Ferrara, Claudia, Bulau, Patrick, Koll, Hans, Mølhøj, Michael

“…Molecular mass determination by electrospray ionization mass spectrometry of a recombinant IgG-based fusion protein (mAb1-F) produced in human embryonic kidney…”
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GingisKHAN™ protease cleavage allows a high-throughput antibody to Fab conversion enabling direct functional assessment during lead identification of human monoclonal and bispecific IgG1 antibodies by Moelleken, Jörg, Endesfelder, Manuel, Gassner, Christian, Lingke, Sabine, Tomaschek, Simone, Tyshchuk, Oksana, Lorenz, Stefan, Reiff, Ulrike, Mølhøj, Michael

“…The determination of the binding strength of immunoglobulins (IgGs) to targets can be influenced by avidity when the targets are soluble di- or multimeric…”
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Characterization and prediction of positional 4-hydroxyproline and sulfotyrosine, two post-translational modifications that can occur at substantial levels in CHO cells-expressed biotherapeutics by Tyshchuk, Oksana, Gstöttner, Christoph, Funk, Dennis, Nicolardi, Simone, Frost, Stefan, Klostermann, Stefan, Becker, Tim, Jolkver, Elena, Schumacher, Felix, Koller, Claudia Ferrara, Völger, Hans Rainer, Wuhrer, Manfred, Bulau, Patrick, Mølhøj, Michael

“…Biotherapeutics may contain a multitude of different post-translational modifications (PTMs) that need to be assessed and possibly monitored and controlled to…”
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Heavy and light chain pairing of bivalent quadroma and knobs-into-holes antibodies analyzed by UHR-ESI-QTOF mass spectrometry by Schaefer, Wolfgang, Völger, Hans R, Lorenz, Stefan, Imhof-Jung, Sabine, Regula, Jörg T, Klein, Christian, Mølhøj, Michael

“…The quadroma antibody represents the first attempt to produce a bispecific heterodimeric IgG antibody by somatic fusion of 2 hybridoma cells each expressing…”
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DuoMab: a novel CrossMab-based IgG-derived antibody format for enhanced antibody-dependent cell-mediated cytotoxicity by Sustmann, Claudio, Dickopf, Steffen, Regula, Jörg T, Kettenberger, Hubert, Mølhøj, Michael, Gassner, Christian, Weininger, Diana, Fenn, Sebastian, Manigold, Tobias, Kling, Lothar, Künkele, Klaus-Peter, Schwaiger, Manfred, Bossenmaier, Birgit, Griese, Julia J, Hopfner, Karl-Peter, Graff-Meyer, Alexandra, Stahlberg, Henning, Ringler, Philippe, Lauer, Matthias E, Brinkmann, Ulrich, Schaefer, Wolfgang, Klein, Christian

“…High specificity accompanied with the ability to recruit immune cells has made recombinant therapeutic antibodies an integral part of drug development. Here we…”
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CD19-/CD3-bispecific antibody of the BiTE class is far superior to tandem diabody with respect to redirected tumor cell lysis by Mølhøj, Michael, Crommer, Sandrine, Brischwein, Klaus, Rau, Doris, Sriskandarajah, Mirnaalini, Hoffmann, Patrick, Kufer, Peter, Hofmeister, Robert, Baeuerle, Patrick A.

“…Many kinds of bispecific antibodies recruiting T cells for cancer therapy have been developed. Side-by-side comparison has shown that CD19-/CD3-bispecific…”
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The Biosynthesis of L-Arabinose in Plants: Molecular Cloning and Characterization of a Golgi-Localized UDP-D-Xylose 4-Epimerase Encoded by the MUR4 Gene of Arabidopsis by Burget, Emilie G., Verma, Rajeev, Mølhøj, Michael, Reiter, Wolf-Dieter

“…The mur4 mutant of Arabidopsis shows a 50% reduction in the monosaccharide L-Ara in leaf-derived cell wall material because of a partial defect in the…”
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The Biosynthesis of D-Galacturonate in Plants. Functional Cloning and Characterization of a Membrane-Anchored UDP-D-Glucuronate 4-Epimerase from Arabidopsis by Mølhøj, Michael, Verma, Rajeev, Reiter, Wolf-Dieter

“…Pectic cell wall polysaccharides owe their high negative charge to the presence of D-galacturonate, a monosaccharide that appears to be present only in plants…”
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The biosynthesis of the branched‐chain sugar d‐apiose in plants: functional cloning and characterization of a UDP‐d‐apiose/UDP‐d‐xylose synthase from Arabidopsis by Mølhøj, Michael, Verma, Rajeev, Reiter, Wolf‐Dieter

“…Summary d‐Apiose is a plant‐specific branched‐chain monosaccharide found in rhamnogalacturonan II (RG‐II), apiogalacturonan, and several apioglycosides. Within…”
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Molecular Analysis of 10 Coding Regions from Arabidopsis That Are Homologous to the MUR3 Xyloglucan Galactosyltransferase by Li, Xuemei, Israel Cordero, Caplan, Jeffrey, Mølhøj, Michael, Reiter, Wolf-Dieter

“…Plant cell walls are composed of a large number of complex polysaccharides, which contain at least 13 different monosaccharides in a multitude of linkages…”
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