Search Results - "Moks, Tomas"

Enhanced in vitro Refolding of Insulin-like Growth Factor I Using a Solubilizing Fusion Partner by Samuelsson, Elisabet, Moks, Tomas, Uhlen, Mathias, Nilsson, Bjoern

“…We have previously shown that human insulin-like growth factor I (IGF-I), fused to ZZ (two domains derived from staphylococcal protein A), can be refolded at…”
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Facilitated In Vitro Refolding of Human Recombinant Insulin-Like Growth Factor I Using a Solubilizing Fusion Partner by Samuelsson, Elisabet, Wadensten, Henrik, Hartmanis, Maris, Moks, Tomas, Uhlén, Mathias

“…We describe a new approach to refolding recombinant proteins in which an affinity fusion partner, consisting of two IgG-binding domains (ZZ) derived from…”
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Large-Scale Affinity Purification of Human Insulin-Like Growth Factor I from Culture Medium of Escherichia Coli by Moks, Tomas, Abrahmsén, Lars, Josephson, Staffan, Östling, Martin, Österlöf, Björn, Enfors, Sven-Olof, Persson, Ingalill, Nilsson, Björn, Uhlén, Mathias

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Expression of human insulin-like growth factor I in bacteria: use of optimized gene fusion vectors to facilitate protein purification by Moks, Tomas, Abrahmsen, Lars, Holmgren, Erik, Bilich, Margareta, Olsson, Anders, Pohl, Gunnar, Sterky, Catharina, Hultberg, Hans, Josephson, Staffan

“…Several fusions between the gene for human insulin-like growth factor I (IGF-I) and the genes for different IgG-binding fragments of staphylococcal protein A…”
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Staphylococcal protein A consists of five IgG‐binding domains by MOKS, Tomas, ABRAHMSÉN, Lars, NILSSON, Björn, HELLMAN, Ulf, SJÖQUIST, John, UHLÉN, Mathias

“…A genetic approach is described to clarify the IgG‐binding properties of the N‐terminal portion of staphylococcal protein A (region E). Several gene fragments,…”
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Immobilization and affinity purification of recombinant proteins using histidine peptide fusions by LJUNGQUIST, Charlotta, BREITHOLTZ, Anna, BRINK‐NILSSON, Helena, MOKS, Tomas, UHLÉN, Mathias, NILSSON, Björn

“…A gene fusion approach to simplify protein immobilization and purification is described. A gene encoding the protein of interest is fused to a gene fragment…”
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Single-Step Recovery of a Secreted Recombinant Protein by Expanded Bed Adsorption by Hansson, Marianne, Ståhl, Stefan, Hjorth, Rolf, Uhlén, Mathias, Moks, Tomas

“…We have used an expanded bed adsorption procedure for efficient recovery of a recombinant fusion protein, directly from a crude fermentor broth without prior…”
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Gene fusions for purpose of expression: an introduction by Uhlén, M, Moks, T

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Fusion proteins in biotechnology by Uhlén, M, Forsberg, G, Moks, T, Hartmanis, M, Nilsson, B

“…Gene fusion techniques allow the production of recombinant proteins featuring the combined characteristics of the parental products. Originally, these…”
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A synthetic IgG-binding domain based on staphylococcal protein A by Nilsson, B, Moks, T, Jansson, B, Abrahmsén, L, Elmblad, A, Holmgren, E, Henrichson, C, Jones, T A, Uhlén, M

“…A synthetic IgG-binding domain based on staphylococcal protein A was designed with the aid of sequence comparisons and computer graphic analysis. A strategy,…”
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Secretion of heterologous gene products to the culture medium of Escherichia coli by Abrahmsén, Lars, Moks, Tomas, Nilsson, Björn, Uhlén, Mathias

“…Different constructs containing fragments of the Staphylococcal protein A gene have been introduced in Escherichia coli and the effect on expression and…”
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Dual Affinity Fusion Approach and Its Use to Express Recombinant Human Insulin-Like Growth Factor II by Hammarberg, Bjorn, Nygren, Per-Ake, Holmgren, Erik, Elmblad, Anette, Tally, Michael, Hellman, Ulf, Moks, Tomas, Uhlen, Mathias

“…A dual affinity fusion concept has been developed in which the gene encoding the desired product is fused between two flanking heterologous genes encoding IgG-…”
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Solid phase DNA sequencing using the biotin-avidin system by STAHL, S, HULTMAN, T, OLSSON, A, MOKS, T, UHLEN, M

“…A novel method for solid-phase DNA sequencing is described. A plasmid vector, pRIT27, has been designed to allow directional immobilization of double stranded…”
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A gene fusion system for generating antibodies against short peptides by Löwenadler, B, Jansson, B, Paleus, S, Holmgren, E, Nilsson, B, Moks, T, Palm, G, Josephson, S, Philipson, L, Uhlén, M

“…A novel method to obtain specific antibodies against short peptides is described, involving synthesis of the corresponding oligodeoxynucleotides followed by…”
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Single‐Step Trypsin Cleavage of a Fusion Protein to Obtain Human Insulin and Its C Peptide by Jonasson, Per, Nilsson, Joakim, Samuelsson, Elisabet, Moks, Tomas, Stårhl, Stefan, Uhlén, Mathias

“…The kinetics for trypsin cleavage of different fusion proteins, consisting of human proinsulin and two IgG‐binding domains (ZZ), were investigated. To achieve…”
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Thiol‐directed immobilization of recombinant IgG‐binding receptors by LJUNGQUIST, Charlotta, JANSSON, Birger, MOKS, Tomas, UHLÉN, Mathias

“…A genetic engineering approach is described for directed immobilization of IgG‐binding receptors to a thiolcontaining matrix using a single cysteine residue…”
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Semi-automated solid-phase DNA sequencing by Uhlén, M, Hultman, T, Wahlberg, J, Lundeberg, J, Bergh, S, Pettersson, B, Holmberg, A, Ståhl, S, Moks, T

“…Increasing the efficiency of DNA sequencing necessitates the development of systems which reduce the need for manual operations by integrating template…”
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Differential stability of recombinant human insulin-like growth factor II in Escherichia coli and Staphylococcus aureus by Hammarberg, B, Moks, T, Tally, M, Elmblad, A, Holmgren, E, Murby, M, Nilsson, B, Josephson, S, Uhlén, M

“…Recombinant human insulin-like growth factor II (IGF-II), produced as a soluble extracellular fusion protein, was shown to be proteolytically degraded in…”
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Characterization of an extended form of recombinant human insulin-like growth factor II by HAMMARBERG, B, TALLY, M, SAMUELSSON, E, WADENSTEN, H, HOLMGREN, E, HARTMANIS, M, HALL, K, UHLEN, M, MOKS, T

“…To investigate the biological role of variants of human insulin-like growth factor II (IGF-II), an extended form designated IGF-IIE21, with a molecular mass of…”
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Protein engineering to optimize recombinant protein purification by Uhlén, M, Moks, T, Abrahmsén, L

“…Genetic approaches have been used to facilitate purification of recombinant proteins, on both a large and a small scale. Based on developments in three…”
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