Search Results - "Mohr, Stephanie E."

Methionine metabolism and methyltransferases in the regulation of aging and lifespan extension across species by Parkhitko, Andrey A., Jouandin, Patrick, Mohr, Stephanie E., Perrimon, Norbert

“…Methionine restriction (MetR) extends lifespan across different species and exerts beneficial effects on metabolic health and inflammatory responses. In…”
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An integrative approach to ortholog prediction for disease-focused and other functional studies by Hu, Yanhui, Flockhart, Ian, Vinayagam, Arunachalam, Bergwitz, Clemens, Berger, Bonnie, Perrimon, Norbert, Mohr, Stephanie E

“…Mapping of orthologous genes among species serves an important role in functional genomics by allowing researchers to develop hypotheses about gene function in…”
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RNAi screening comes of age: improved techniques and complementary approaches by Mohr, Stephanie E., Smith, Jennifer A., Shamu, Caroline E., Neumüller, Ralph A., Perrimon, Norbert

“…Key Points RNAi has been used for genome-wide screening and other studies that aim to uncover the function of genes and gene networks. Sequence-specific RNAi…”
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FlyRNAi.org 2025 update-expanded resources for new technologies and species by Hu, Yanhui, Comjean, Aram, Rodiger, Jonathan, Chen, Weihang, Kim, Ah-Ram, Qadiri, Mujeeb, Gao, Chenxi, Zirin, Jonathan, Mohr, Stephanie E, Perrimon, Norbert

“…The design, analysis and mining of large-scale 'omics studies with the goal of advancing biological and biomedical understanding require use of a range of…”
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Loss-of-function genetic tools for animal models: cross-species and cross-platform differences by Housden, Benjamin E., Muhar, Matthias, Gemberling, Matthew, Gersbach, Charles A., Stainier, Didier Y. R., Seydoux, Geraldine, Mohr, Stephanie E., Zuber, Johannes, Perrimon, Norbert

“…Key Points Loss-of-function (LOF) technologies are widely used across many model organisms and many fields. Although all LOF approaches have the shared goal of…”
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Molecular Interaction Search Tool (MIST): an integrated resource for mining gene and protein interaction data by Hu, Yanhui, Vinayagam, Arunachalam, Nand, Ankita, Comjean, Aram, Chung, Verena, Hao, Tong, Mohr, Stephanie E, Perrimon, Norbert

“…Abstract Model organism and human databases are rich with information about genetic and physical interactions. These data can be used to interpret and guide…”
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Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9 by Ren, Xingjie, Sun, Jin, Housden, Benjamin E., Hu, Yanhui, Roesel, Charles, Lin, Shuailiang, Liu, Lu-Ping, Yang, Zhihao, Mao, Decai, Sun, Lingzhu, Wu, Qujie, Jie, Jun-Yuan, Xi, Jianzhong, Mohr, Stephanie E., Xu, Jiang, Perrimon, Norbert, Ni, Jian-Quan

“…The ability to engineer genomes in a specific, systematic, and cost-effective way is critical for functional genomic studies. Recent advances using the…”
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The Transgenic RNAi Project at Harvard Medical School: Resources and Validation by Perkins, Lizabeth A, Holderbaum, Laura, Tao, Rong, Hu, Yanhui, Sopko, Richelle, McCall, Kim, Yang-Zhou, Donghui, Flockhart, Ian, Binari, Richard, Shim, Hye-Seok, Miller, Audrey, Housden, Amy, Foos, Marianna, Randkelv, Sakara, Kelley, Colleen, Namgyal, Pema, Villalta, Christians, Liu, Lu-Ping, Jiang, Xia, Huan-Huan, Qiao, Wang, Xia, Fujiyama, Asao, Toyoda, Atsushi, Ayers, Kathleen, Blum, Allison, Czech, Benjamin, Neumuller, Ralph, Yan, Dong, Cavallaro, Amanda, Hibbard, Karen, Hall, Don, Cooley, Lynn, Hannon, Gregory J, Lehmann, Ruth, Parks, Annette, Mohr, Stephanie E, Ueda, Ryu, Kondo, Shu, Ni, Jian-Quan, Perrimon, Norbert

“…To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along…”
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CRISPR guide RNA design for research applications by Mohr, Stephanie E., Hu, Yanhui, Ewen‐Campen, Benjamin, Housden, Benjamin E., Viswanatha, Raghuvir, Perrimon, Norbert

“…The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools…”
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A gene-specific T2A-GAL4 library for Drosophila by Lee, Pei-Tseng, Zirin, Jonathan, Kanca, Oguz, Lin, Wen-Wen, Schulze, Karen L, Li-Kroeger, David, Tao, Rong, Devereaux, Colby, Hu, Yanhui, Chung, Verena, Fang, Ying, He, Yuchun, Pan, Hongling, Ge, Ming, Zuo, Zhongyuan, Housden, Benjamin E, Mohr, Stephanie E, Yamamoto, Shinya, Levis, Robert W, Spradling, Allan C, Perrimon, Norbert, Bellen, Hugo J

“…We generated a library of ~1000 stocks in which we inserted a construct in the intron of genes allowing expression of under control of endogenous promoters…”
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RNAi screening: new approaches, understandings, and organisms by Mohr, Stephanie E., Perrimon, Norbert

“…RNA interference (RNAi) leads to sequence‐specific knockdown of gene function. The approach can be used in large‐scale screens to interrogate function in…”
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Large-Scale Transgenic Drosophila Resource Collections for Loss- and Gain-of-Function Studies by Zirin, Jonathan, Hu, Yanhui, Liu, Luping, Yang-Zhou, Donghui, Colbeth, Ryan, Yan, Dong, Ewen-Campen, Ben, Tao, Rong, Vogt, Eric, VanNest, Sara, Cavers, Cooper, Villalta, Christians, Comjean, Aram, Sun, Jin, Wang, Xia, Jia, Yu, Zhu, Ruibao, Peng, Ping, Yu, Jinchao, Shen, Da, Qiu, Yuhao, Ayisi, Limmond, Ragoowansi, Henna, Fenton, Ethan, Efrem, Senait, Parks, Annette, Saito, Kuniaki, Kondo, Shu, Perkins, Liz, Mohr, Stephanie E, Ni, Jianquan, Perrimon, Norbert

“…Abstract The Transgenic RNAi Project (TRiP), a Drosophila melanogaster functional genomics platform at Harvard Medical School, was initiated in 2008 to…”
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Coordination of tumor growth and host wasting by tumor-derived Upd3 by Ding, Guangming, Xiang, Xiaoxiang, Hu, Yanhui, Xiao, Gen, Chen, Yuchen, Binari, Richard, Comjean, Aram, Li, Jiaying, Rushworth, Elisabeth, Fu, Zhenming, Mohr, Stephanie E., Perrimon, Norbert, Song, Wei

“…yki-induced gut tumors in Drosophila are associated with host wasting, including muscle dysfunction, lipid loss, and hyperglycemia, a condition reminiscent of…”
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Drosophila melanogaster : a simple system for understanding complexity by Mohr, Stephanie E, Perrimon, Norbert

“…Understanding human gene function is fundamental to understanding and treating diseases. Research using the model organism benefits from a wealth of molecular…”
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An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms by Kanca, Oguz, Zirin, Jonathan, Garcia-Marques, Jorge, Knight, Shannon Marie, Yang-Zhou, Donghui, Amador, Gabriel, Chung, Hyunglok, Zuo, Zhongyuan, Ma, Liwen, He, Yuchun, Lin, Wen-Wen, Fang, Ying, Ge, Ming, Yamamoto, Shinya, Schulze, Karen L, Hu, Yanhui, Spradling, Allan C, Mohr, Stephanie E, Perrimon, Norbert, Bellen, Hugo J

“…We previously reported a CRISPR-mediated knock-in strategy into introns of genes, generating an transgenic library for multiple uses (Lee et al., 2018a). The…”
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An Integrative Analysis of the InR/PI3K/Akt Network Identifies the Dynamic Response to Insulin Signaling by Vinayagam, Arunachalam, Kulkarni, Meghana M., Sopko, Richelle, Sun, Xiaoyun, Hu, Yanhui, Nand, Ankita, Villalta, Christians, Moghimi, Ahmadali, Yang, Xuemei, Mohr, Stephanie E., Hong, Pengyu, Asara, John M., Perrimon, Norbert

“…Insulin regulates an essential conserved signaling pathway affecting growth, proliferation, and metabolism. To expand our understanding of the insulin pathway,…”
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Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophila cells by Xia, Baolong, Viswanatha, Raghuvir, Hu, Yanhui, Mohr, Stephanie E, Perrimon, Norbert

“…Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In cells, while genome-wide loss-of-function screens…”
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Protein complex-based analysis framework for high-throughput data sets by Vinayagam, Arunachalam, Hu, Yanhui, Kulkarni, Meghana, Roesel, Charles, Sopko, Richelle, Mohr, Stephanie E, Perrimon, Norbert

“…Analysis of high-throughput data increasingly relies on pathway annotation and functional information derived from Gene Ontology. This approach has…”
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Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos by Viswanatha, Raghuvir, Mameli, Enzo, Rodiger, Jonathan, Merckaert, Pierre, Feitosa-Suntheimer, Fabiana, Colpitts, Tonya M., Mohr, Stephanie E., Hu, Yanhui, Perrimon, Norbert

“…Mosquito-borne diseases present a worldwide public health burden. Current efforts to understand and counteract them have been aided by the use of cultured…”
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SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing by Chen, Chiao-Lin, Rodiger, Jonathan, Chung, Verena, Viswanatha, Raghuvir, Mohr, Stephanie E, Hu, Yanhui, Perrimon, Norbert

“…CRISPR-Cas9 is a powerful genome editing technology in which a single guide RNA (sgRNA) confers target site specificity to achieve Cas9-mediated genome…”
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