Enzymatic esterification of fatty acid esters by tetraethylammonium amino acid ionic liquids-coated Candida rugosa lipase

[Display omitted] ► New tetraethylammonium amino acids ILs were used as a modifier with Candida rugosa lipase to produce two new biocatalysts (ILs-CRL). ► The activity of ILs-CRL was determined through esterification of oleyl alcohol with different fatty acids. ► The ILs-CRL system showed higher act...

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Bibliographic Details
Published in:	Journal of molecular catalysis. B, Enzymatic Vol. 79; pp. 61 - 65
Main Authors:	Abdul Rahman, Mohd Basyaruddin, Jumbri, Khairulazhar, Mohd Ali Hanafiah, Nurul Ain, Abdulmalek, Emilia, Tejo, Bimo Ario, Basri, Mahiran, Salleh, Abu Bakar
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 01-07-2012 Elsevier
Subjects:	Amino acid ionic liquids amino acids Biocatalyst biocatalysts Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Candida rugosa Candida rugosa lipase catalytic activity Coated-enzyme Esterification fatty acid esters Fundamental and applied biological sciences. Psychology hexane hydrogen bonding hydrophilicity interleukin-2 medium chain fatty acids Methods. Procedures. Technologies mixing oleyl alcohols triacylglycerol lipase Coated-enzyme Amino acid ionic liquids Candida rugosa lipase Esterification Biocatalyst Yeast Enzyme Fatty ester Triacylglycerol lipase Esterases Fatty acids Carboxylic ester hydrolases Ionic liquid Fungi Aminoacid Hydrolases Fungi Imperfecti Candida rugosa Enzymatic reaction
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Summary:	[Display omitted] ► New tetraethylammonium amino acids ILs were used as a modifier with Candida rugosa lipase to produce two new biocatalysts (ILs-CRL). ► The activity of ILs-CRL was determined through esterification of oleyl alcohol with different fatty acids. ► The ILs-CRL system showed higher activity on short, medium and long chain fatty acids compared to native CRL. ► Hydrophilicity of ILs may play an important role to stabilize the ILs-CRL. Enzymatic production of fatty acid esters from the esterification of oleyl alcohol with various fatty acids was investigated by using two new tetraethylammonium amino acid ionic liquids-coated Candida rugosa lipase (ILs-CRL) as biocatalysts in hexane. Both enzyme derivatives were prepared by mixing Candida rugosa lipase with tetraethylammonium l-histidinate (IL1) and tetraethylammonium l-asparaginate (IL2). The ILs-CRL system containing the equivalent protein concentration as in CRL showed higher esterification activity especially on medium chain fatty acids (C12–C16) as compared to non-coated CRL. Hydrophilicity of ILs may play an important role in hydrogen bonding with enzyme surface and consequently stabilize the ILs-CRL.
Bibliography:	http://dx.doi.org/10.1016/j.molcatb.2012.03.003
ISSN:	1381-1177 1873-3158
DOI:	10.1016/j.molcatb.2012.03.003