In vitro and in vivo antioxidant activity of Iraqi propolis against benign prostatic hyperplasia in rats

The objective of the present work included evaluation of antioxidant action of Iraqi propolis extract in vivo and in vitro. The in vitro part of the study comprised studying of the scavenging activity of serial concentrations of propolis extract in two different methods DpPH scavenging assay and red...

Full description

Saved in:

Bibliographic Details
Published in:	Eurasian journal of biosciences Vol. 14; no. 2; pp. 7467 - 7472
Main Authors:	Abd-Alhassen, Jamela K, Mohammed, Ishtar Adnan, Daham, Abbas Fadhil
Format:	Journal Article
Language:	English
Published:	Izmir Foundation for Environmental Protection and Research, Çevre Koruma ve Araştırma Vakfı (ÇEVKOR) 01-01-2020
Subjects:	Acids Antioxidants Ascorbic acid Catalase Experiments Free radicals Glutathione Hyperplasia Malondialdehyde Medical research Oxidative stress Propolis Prostate Superoxide dismutase Testosterone Iraq
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The objective of the present work included evaluation of antioxidant action of Iraqi propolis extract in vivo and in vitro. The in vitro part of the study comprised studying of the scavenging activity of serial concentrations of propolis extract in two different methods DpPH scavenging assay and reducing power assay. Results showed that IC50 of the propolis extract, and the reference drug (ascorbic acid) with regard to the DPPH scavenging activity, were 1.65µg/mL, 1.74 respectively while 1.08 µg/ml and 1.11 µg/ml regarding to the reducing power assay respectively. The in vivo part involved studying the antioxidant and therapeutic activity of 200mg/ kg BW of propolis extract given twice daily on the exogenous testosterone induced benign prostatic hyperplasia (TIBPH) in the wister rat which conducted on 18 male rats divided randomly to the 3 groups of 6 rats for each group. After completion of treatment Prostate weight, prostate index, Malondialdehyde (MDA) content, catalase (CAT) activity, glutathione (GSH) content, and superoxide dismutase (SOD) activity markers were measured. Results showed a significant (P<0.05) improvement in different mentioned parameters of propolis treated rats included reduction in both prostatic weight and prostatic index, decreased in MDA content with significant (P<0.05) increase in GSH content, CAT and SOD activities as compared with positive control group. In conclusion, the above results proved that the propolis have a good therapeutic and antioxidant activities against (TIBPH) in rats.
ISSN:	1307-9867