Model-based Analysis for Real-time Label-free Ultraviolet Quantification of Ultrafast Plasmonic Polymerase Chain Reaction

Model-based Analysis for Real-time Label-free Ultraviolet Quantification of Ultrafast Plasmonic Polymerase Chain Reaction

A mathematical model for DNA quantification was calibrated using experimental results from real-time 260nm absorption measurements of plasmonic PCR thermocycling. The effect of different PCR parameters on template amplification was investigated using the calibrated model.

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Bibliographic Details
Published in:2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC) pp. 5136 - 5139
Main Authors: Mohammadyousef, P., Paliouras, M., Trifiro, M., Kirk, A.G.
Format: Conference Proceeding
Language:English
Published: IEEE 01-07-2020
Online Access:Get full text
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Description
Summary:A mathematical model for DNA quantification was calibrated using experimental results from real-time 260nm absorption measurements of plasmonic PCR thermocycling. The effect of different PCR parameters on template amplification was investigated using the calibrated model.
ISSN:1558-4615
DOI:10.1109/EMBC44109.2020.9176669