Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using Streptomyces sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against P. aeruginosa USM-AR2 and MRSA

Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel...

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Published in:	Molecules (Basel, Switzerland) Vol. 26; no. 21; p. 6414
Main Authors:	Murugaiah, Hemalatha, Teh, Chow Lun, Loh, Kai Chew, Mohamad Yahya, Ahmad Ramli, Md Noh, Nur Asshifa, Abu Bakar, Noor Hana Hanif, Kernain, Daruliza, Hashim, Rokiah, Bustami, Yazmin
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 24-10-2021 MDPI
Subjects:	Alginates Alginic acid Anti-Bacterial Agents - biosynthesis Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology antibacterial Antibiotics anticancer Antineoplastic Agents - chemistry Antineoplastic Agents - metabolism Antineoplastic Agents - pharmacology Bacteria Biofilms Biosynthesis Cancer Cell Line, Tumor Cell Proliferation - drug effects Cellulose cellulose nanocrystals Crystals Cytotoxicity Drug resistance Drug Screening Assays, Antitumor Enzymes Fourier transforms Humans Hydrogels Hydrogels - chemical synthesis Hydrogels - chemistry Hydrogels - pharmacology Infrared spectroscopy Metabolites Metal Nanoparticles - chemistry Methicillin-Resistant Staphylococcus aureus - drug effects Microbial Sensitivity Tests Minimum inhibitory concentration Morphology Nanocrystals Nanoparticles Proteins Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Silver Silver - chemistry Silver - metabolism Silver - pharmacology silver nanoparticles Streptomyces Streptomyces - chemistry Streptomyces - metabolism Streptomyces sp Tumors Wound healing Wound infection alginate wound healing silver nanoparticles Streptomyces sp biosynthesis antibacterial anticancer cellulose nanocrystals
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Summary:	Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel film. sp. PBD-311B was used for the biosynthesis of AgNPs. The synthesized bioAgNPs were characterized using UV-Vis spectroscopy, TEM, XRD, and FTIR analysis. Then, the bioAgNPs' antibacterial and anticancer properties were determined using TEMA and cytotoxicity analysis. To form the antibacterial hydrogel film, bioAgNPs were mixed with a CNC and Alg solution and further characterized using FTIR analysis and a disc diffusion test. The average size of the synthesized bioAgNPs is around 69 ± 2 nm with a spherical shape. XRD analysis confirmed the formation of silver nanocrystals. FTIR analysis showed the presence of protein capping at the bioAgNP surface and could be attributed to the extracellular protein binding to bioAgNPs. The MIC value of bioAgNPs against USM-AR2 and MRSA was 6.25 mg/mL and 3.13 mg/mL, respectively. In addition, the bioAgNPs displayed cytotoxicity effects against cancer cells (DBTRG-0.5MG and MCF-7) and showed minimal effects against normal cells (SVG-p12 and MCF-10A), conferring selective toxicity. Interestingly, the bioAgNPs still exhibited inhibition activity when incorporated into CNC/Alg, which implies that the hydrogel film has antibacterial properties. It was also found that bioAgNP-CNC/Alg displayed a minimal or slow release of bioAgNPs owing to the intermolecular interaction and the hydrogel's properties. Overall, bioAgNP-CNC/Alg is a promising antibacterial hydrogel film that showed inhibition against the pathogenic bacteria and MRSA and its application can be further evaluated for the inhibition of cancer cells. It showed benefits for surgical resection of a tumor to avoid post-operative wound infection and tumor recurrence at the surgical site.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1420-3049 1420-3049
DOI:	10.3390/molecules26216414