A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol

A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol

A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilizati...

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Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Vol. 10; no. 7; p. 1346
Main Authors: Mat Zaid, Mohd Hazani, Abdullah, Jaafar, Rozi, Normazida, Mohamad Rozlan, Aliff Aiman, Abu Hanifah, Sharina
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 10-07-2020
MDPI
Subjects:
17ß-estradiol
17β-Estradiol
Absorption spectra
aptamer
Aptamers
aptasensor
Biosensors
Bisphenol A
Carbon
carbon nanodots
Chromatography
Electrochemical analysis
Electrochemical impedance spectroscopy
Electrochemistry
Electrodes
Electrolytes
Fluorescence
Fourier transforms
Immobilization
impedance analysis
Infrared spectroscopy
Morphology
Nanomaterials
Potassium
Progesterone
Rivers
Selectivity
Sensors
Sex hormones
Signal transduction
Spectrum analysis
Transmission electron microscopy
Voltammetry
Water analysis
Water sampling
Selangor Malaysia
Malaysia
carbon nanodots
impedance analysis
17ß-estradiol
aptamer
aptasensor
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Summary:A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilization of 76-mer aptamer probe. The morphology and structure of the nanomaterial were characterized by UV-visible absorption spectra, Fluorescence spectra, Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Moreover, cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the electrochemical performance of the prepared electrodes. Subsequently, impedimetric (EIS) measurements were employed to investigate the relative impedances changes before and after E2 binding, which results in a linear relationship of E2 concentration in the range of 1.0 × 10 to 1.0 × 10 M, with a detection limit of 0.5 × 10 M. Moreover, the developed biosensor showed high selectivity toward E2 and exhibited excellent discrimination against progesterone (PRG), estriol (E3) and bisphenol A (BPA), respectively. Moreover, the average recovery rate of spiked river water samples with E2 ranged from 98.2% to 103.8%, with relative standard deviations between 1.1% and 3.8%, revealing the potential application of the present biosensor for E2 detection in water samples.
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ISSN:2079-4991
2079-4991
DOI:10.3390/nano10071346