Age-induced perturbation in cell membrane phospholipid fatty acid profile of longevity-selected Drosophila melanogaster and corresponding control lines
Various compositions of fatty acids can produce cell membranes with disparate fluidity and propensity for oxidation. The latter characteristic, which can be evaluated via the peroxidation index (PI), has a fundamental role in the development of the “membrane-pacemaker theory” of aging. This study tr...
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| Published in: | Experimental gerontology Vol. 48; no. 11; pp. 1362 - 1368 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
Elsevier Inc
01-11-2013
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Various compositions of fatty acids can produce cell membranes with disparate fluidity and propensity for oxidation. The latter characteristic, which can be evaluated via the peroxidation index (PI), has a fundamental role in the development of the “membrane-pacemaker theory” of aging. This study tried to evaluate differences between the membrane phospholipid fatty acid (PLFA) profile of longevity-selected (L) and corresponding control (C) lines of Drosophila melanogaster with age (3, 9, 14 and 19days) and its consequences on phase transition temperature as a function of membrane fluidity. Despite an equal proportion of polyunsaturated fatty acids, PI and double bond index over all ages in both experimental groups, monounsaturated fatty acids showed significant variation with advancement of age in both L and C lines. A significant age-associated elevation of the unsaturation vs. saturation index in parallel with a gradual reduction of the mean melting point was observed in longevous flies. PLFA composition of the L vs. C lines revealed a dissimilarity in 3-day old samples, which was based on the positive loading of C14:0 and C18:3 as well as negative loading of C18:0. The findings of this study are not in agreement with the principle of the “membrane pacemaker theory” linking PI and longevity. However, the physiochemical properties of PLFAs in longevity lines may retard the cells' senescence by maintaining optimal membrane functionality over time. Identical susceptibility to peroxidation of both types of lines underlines the involvement of other mechanisms in protecting the bio-membrane against oxidation, such as the reduced production of mitochondrial reactive oxygen species or improvement of the antioxidant defense system in longer-lived phenotypes. Concurrent assessments of these mechanisms in relation to cell membrane PLFA composition may clarify the cellular basis of lifespan in this species.
•We assessed the effect of longevity selection and age on cell phospholipid profile.•Proportion of polyunsaturated fatty acids was equal in longevity and control lines.•The proportion of monounsaturated fatty acids increased in both lines with age.•This study does not support linking peroxidation index to longevity. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0531-5565 1873-6815 |
| DOI: | 10.1016/j.exger.2013.08.018 |