Nitric oxide (NO) production in mammalian non-tumorigenic epithelial cells of the small intestine and macrophages induced by individual strains of lactobacilli and bifidobacteria

Nitric oxide (NO) production in mammalian non-tumorigenic epithelial cells of the small intestine and macrophages induced by individual strains of lactobacilli and bifidobacteria

Nitric oxide (NO) affects multiple gastrointestinal functions, including mucosal inflammation and antimicrobial activity. The aim of this study was to screen the ability of probiotic bacteria to stimulate NO production in porcine intestinal epithelial cells and macrophages in the presence and absenc...

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Bibliographic Details
Published in:International dairy journal Vol. 19; no. 3; pp. 166 - 171
Main Authors: Pipenbaher, Nataša, Moeller, Peter Lange, Dolinšek, Jan, Jakobsen, Mogens, Weingartl, Hana, Cencič, Avrelija
Format: Journal Article
Language:English
Published: Oxford Elsevier Ltd 01-03-2009
Elsevier
Subjects:
Biological and medical sciences
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Milk and cheese industries. Ice creams
Lactic acid bacteria
Individual
Nitric oxide
Dairy industry
Production
Bacteria
Lactobacillaceae
Epithelial cell
Small intestine
Lactobacillus
Strain
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Summary:Nitric oxide (NO) affects multiple gastrointestinal functions, including mucosal inflammation and antimicrobial activity. The aim of this study was to screen the ability of probiotic bacteria to stimulate NO production in porcine intestinal epithelial cells and macrophages in the presence and absence of interferon gamma (INF-γ). Production of NO in intestinal epithelium was stimulated by individual strains of lactobacilli without INF-γ priming. While none of the tested bifidobacteria were capable of inducing NO production, most constitutively secreted NO. Most tested strains induced a significant increase in NO production compared with the control cells in the macrophage cell line 3D4/21. Results support the protective role of the individual strains of the genera Lactobacillus and Bifidobacterium and may lead to new approaches for manipulating and regulating immune responses at the mucosal surfaces of the gastrointestinal tract.
ISSN:0958-6946
1879-0143
DOI:10.1016/j.idairyj.2008.09.003