Search Results - "Model, M. A"
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Experimental test of the geometric model of image formation in bright‐field microscopy
Published in Journal of microscopy (Oxford) (01-07-2021)“…In the geometric optics approximation, an image formed by an objective lens replicates the distribution of intensity at the front focal plane of the objective…”
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2
Measurement of protein concentration in bacteria and small organelles under a light transmission microscope
Published in Journal of molecular recognition (01-09-2024)“…Protein concentration (PC) is an essential characteristic of cells and organelles; it determines the extent of macromolecular crowding effects and serves as a…”
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3
Selective Visualization of Live Intestinal Parasites in Stool Specimens Without Purification
Published in Acta parasitologica (01-09-2022)“…Purpose Microscopic observation of live parasites in the stool is an important diagnostic tool in human and veterinary medicine. Because of the presence of…”
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A model for membrane potential and intracellular ion distribution
Published in Chemistry and physics of lipids (01-12-2014)“…•Intracellular concentration profile of ions for cells with negative Donnan potential is derived.•Donnan potential is produced by ion depletion within a narrow…”
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5
Concentrated dyes as a source of two-dimensional fluorescent field for characterization of a confocal microscope
Published in Journal of microscopy (Oxford) (2008)“…The axial spread function is a useful tool for evaluation of a confocal microscope. It can be obtained experimentally by scanning a uniform fluorescent layer…”
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Measurement of the absorption of concentrated dyes and their use for quantitative imaging of surface topography
Published in Journal of microscopy (Oxford) (01-07-2008)“…We propose a method to image the surface topography of transparent objects. The space between the object and the opposite closely positioned surface (such as a…”
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3D deconvolution of spherically aberrated images using commercial software
Published in Journal of microscopy (Oxford) (01-01-2011)“…Refractive index mismatch between the specimen and the objective immersion oil results in spherical aberration, which causes distortion and spreading of the…”
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Ligand-receptor interaction rates in the presence of convective mass transport
Published in Biophysical journal (01-11-1995)“…The rate of binding of a ligand to receptors on the cell surface can be diffusion limited. We analyze the kinetics of binding, diffusion-limited in a…”
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Desensitization of the actin polymerization response in human neutrophils at low cell density
Published in Journal of leukocyte biology (01-09-1995)“…Many chemoattractant-activated responses in neutrophils show transient kinetics, suggesting that rapid desensitization occurs during the time course of the…”
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10
Bright-field microscopy of transparent objects: a ray tracing approach
Published 15-02-2017“…Formation of a bright-field microscopic image of a transparent phase object is described in terms of elementary geometrical optics. Our approach is based on…”
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11
Quantification of the surface density of a fluorescent label with the optical microscope
Published in Journal of biomedical materials research (01-04-2000)“…Fluorescence microscopy can offer unique advantages for biomaterials characterization. Like spectroscopy or radioactivity, it can be used to quantify specific…”
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Optimization of the Cost and Sensitivity of Receptor- and Enzyme-Based Assays
Published in Analytical biochemistry (15-06-1999)“…In detecting receptor antagonists or enzyme inhibitors, there are three parameters that often affect the outcome in a predictable quantitative manner:…”
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13
Cell Polarization as a Possible Mechanism of Response Termination
Published in Biochemical and biophysical research communications (16-07-1996)“…Neutrophils stimulated with a low concentration of chemotactic peptide N-formyl-met-leu-phe respond with a brief pulse of actin polymerization and a persistent…”
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EXPERIMENTAL APPROACHES FOR OBSERVING HOMOLOGOUS DESENSITISATION AND THEIR PITFALLS
Published in Pharmacological research (01-04-1998)“…Whereas molecular mechanisms of cell desensitisation have been discussed at length in the literature, little organised information on the methods for studying…”
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EXPERIMENTAL APPROACHES FOR OBSERVING HOMOLOGOUS DESENSITISATION AND THEIR PITFALLS
Published in Pharmacological research (01-07-1998)“…Whereas molecular mechanisms of cell desensitisation have been discussed at length in the literature, little organised information on the methods for studying…”
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16
A sensitive flow cytometric method for measuring the oxidative burst
Published in Journal of immunological methods (28-03-1997)“…We report a novel method of flow cytometric detection of the oxidative burst in human neutrophils. The cells were covalently labeled on the plasma membrane by…”
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17
A Microscopic Study of FcγRIII-Mediated Respiratory Burst in Neutrophils
Published in Immunobiology (1979) (01-07-1998)“…We investigated the relationship between the respiratory burst in neutrophils and the membrane distribution of the IgG receptor, FcγRIII.FcγRIII receptors were…”
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18
A standard for calibration and shading correction of a fluorescence microscope
Published in Cytometry (New York, N.Y.) (01-08-2001)“…Background Numerous applications of fluorescence microscopy require quantitation of signal intensity in reproducible units. Two problems must be overcome to…”
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19
Determination of glutathione peroxidase activity
Published in Voprosy meditsinskoi khimii (01-07-1989)“…A simplified procedure is developed for estimation of glutathione peroxidase activity involving glutathione reductase in the conjugated reaction. The procedure…”
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