Search Results - "Moazen, Fatemeh"
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Cloning and Optimization of Intracellular Expression of Human Interferon β-1a in Pichia pastoris GS115
Published in Advanced biomedical research (01-08-2024)“…Interferon-beta (IFN-β) is a cytokine with a wide range of biological and pharmaceutical applications, including multiple sclerosis (MS), cancer, some…”
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Rational design of a new variant of Reteplase with optimized physicochemical profile and large-scale production in Escherichia coli
Published in World journal of microbiology & biotechnology (01-02-2022)“…Structural engineering of the recombinant thrombolytic drug, Reteplase, and its cost-effective production are important goals in the pharmaceutical industry…”
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Optimization of solvent media to solubilize TEV protease using response surface method
Published in Research in pharmaceutical sciences (01-07-2020)“…Background and purpose: Tobacco etch virus (TEV) protease, a 27 KDa protein, consists of the catalytic domain of nuclear inclusion a (NIa) protein produced by…”
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Cloning, optimization of periplasmic expression and purification of recombinant granulocyte macrophage-stimulating factor in Escherichia coli BL21 (DE3)
Published in Advanced biomedical research (01-01-2019)“…Background: Molgramostim, a nonglycosylated version of recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF), can be produced in a high level…”
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Twin arginine translocation system in secretory expression of recombinant human growth hormone
Published in Research in pharmaceutical sciences (01-11-2016)“…Recombinant protein production in E. coli has several advantages over other expression systems. Misfolding, inclusion body formation, and lack of eukaryotic…”
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Optimization of the expression of phaC2 encoding poly (3-hydroxyalkanoate) synthase from Pseudomonas aeruginosa PTCC1310 in Fad B deleted Escherichia coli
Published in Advanced biomedical research (01-01-2016)“…Background: Poly3-hydroxyalkanoates (PHAs) are potential candidates for the industrial production of biodegradable plastics. Therefore, in the present study,…”
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Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients
Published in Advanced biomedical research (2015)“…Type 2 diabetes (T2D) is a multifactorial disease with susceptibility of several genes that are related to T2D. Insulin secretion pathway starts with potassium…”
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Polymorphism of Apo lipoprotein E gene and the risk of multiple sclerosis
Published in Journal of research in medical sciences (01-12-2011)“…Background: Apolipoprotein E (ApoE) gene encodes an important protein in reforming injuries of central nervous system (CNS). It is assumed that various ApoE…”
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Optimization of the Production and Purification of Taq Polymerase Enzyme Containing N666E Mutation in its O-Helix Region
Published in Majallah-i dānishkadah-i pizishkī-i Iṣfahān. (Online) (01-08-2012)“…Background: The aim of this project was to isolate and purify the highly active recombinant Taq DNA polymerase from the strain of Escherichia coli BL21. This…”
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Polymerase chain reaction amplification of a GC rich region by adding 1,2 propanediol
Published in Advanced biomedical research (2014)“…Apolipoprotein E (ApoE) is one of the most important carriers of lipids in mammalians. The gene for this lipoprotein (ApoE) is located on chromosome 19 which…”
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Improvement of soluble expression of GM-CSF in the cytoplasm of Escherichia coli using chemical and molecular chaperones
Published in Protein expression and purification (01-08-2019)“…The most common approaches to improve soluble expression of heterologous proteins are applications of molecular chaperones such as DnaK, DnaJ, GrpE, GroEL and…”
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Optimization of the Production of Soluble Recombinant TEV Protease in Two E. coli Strains
Published in Avicenna journal of medical biotechnology (01-10-2024)“…Background: The low solubility of Tobacco Etch Virus (TEV) protease, a functional enzyme that cleaves protein tags without significant modification in its…”
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Recombinant Production and Intein-Mediated Purification of an Antimicrobial Peptide, BR2
Published in International journal of peptide research and therapeutics (01-12-2017)“…Nowadays, targeted therapy of cancer is under intensive focus of many investigations due to severe side effects imposed by various cancer chemotherapeutics…”
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High-yield Production of Granulocyte-macrophage Colony-stimulating Factor in E. coli BL21 (DE3) By an Auto-induction Strategy
Published in Iranian journal of pharmaceutical research : IJPR (01-01-2019)“…A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to…”
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Efficient Media for High Lipase Production: One Variable at a Time Approach
Published in Avicenna journal of medical biotechnology (01-04-2017)“…Lipase enzymes have applications in a wide range of industries. A crucial determining factor of industrial prices of these enzymes is the culture media…”
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Isolation and characterization of novel thermophilic lipase-secreting bacteria
Published in Brazilian journal of microbiology (01-12-2013)“…The purpose of the present study was to screen and identify the lipase-producing microorganisms from various regions of Iran. Samples collected from hot…”
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Optimization of the Expression of Reteplase in Escherichia coli TOP10 Using Arabinose Promoter
Published in Jundishapur journal of natural pharmaceutical products (20-02-2015)Get full text
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COMPARISON OF THE CYTOPLASMIC AND PERIPLASMIC PRODUCTION OF RETEPLASE IN Escherichia coli
Published in Preparative biochemistry & biotechnology (03-10-2013)“…Reteplase is the recombinant type of tissue plasminogen activator variant. In this study, preplasmic and cytoplasmic (as inclusion body: IBs) production and…”
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Isolation and Characterization of a New Thermoalkalophilic Lipase from Soil Bacteria
Published in Iranian journal of pharmaceutical research : IJPR (01-01-2015)“…Lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. In this…”
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