A comparative study on the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa

A comparative study on the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa

These cross-sectional studies reported the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa. A total of 328 samples (faeces, feeds, silage, and drinking water) were collected fro...

Full description

Saved in:
Bibliographic Details
Published in:Tropical animal health and production Vol. 56; no. 2; p. 88
Main Authors: Gana, J., Gcebe, N., Moerane, R., Ngoshe, Y. B., Tshuma, T., Moabelo, K., Adesiyun, A. A.
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-03-2024
Springer Nature B.V
Subjects:
Abattoirs
Animals
Beef
Biomedical and Life Sciences
Cattle
Comparative analysis
Comparative studies
Cross-Sectional Studies
Drinking water
Farms
Feces
Female
Food Microbiology
Food safety
Genes
Geographical distribution
Life Sciences
Listeria
Listeria - genetics
Listeria innocua
Listeria monocytogenes
Listeria welshimeri
Livestock farming
Pathogens
Polymerase chain reaction
Regular
Regular Articles
South Africa - epidemiology
Species
Veterinary Medicine/Veterinary Science
Virulence
Zoology
South Africa
Abattoirs
Virulence genes
Serogroups
Cattle farms
Listeria
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:These cross-sectional studies reported the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa. A total of 328 samples (faeces, feeds, silage, and drinking water) were collected from 23 cattle farms (communal, cow-calf, and feedlot), and 262 samples (faeces, carcass swabs, and effluents) from 8 beef abattoirs (low throughput and high throughput) were processed using standard bacteriological and molecular methods to detect Listeria species. The factors associated with the prevalence of Listeria species were investigated, and multiplex polymerase chain reaction (mPCR) was used to determine Listeria species, the pathogenic serogroups, and the carriage of eight virulence-associated genes by Listeria monocytogenes . The overall prevalence of Listeria species in cattle farms was 14.6%, comprising Listeria innocua (11.3%), Listeria monocytogenes (3.4%), Listeria welshimeri (0.0%) compared with 11.1%, comprising Listeria innocua (5.7%), Listeria monocytogenes (4.6%), Listeria welshimeri (0.8%) for beef abattoirs. Of the three variables (area, type of farm/abattoir, and sample type) investigated, only the sample types at abattoirs had a significant ( P  < 0.001) effect on the prevalence of L. innocua and L. welshimeri. The frequency of distribution of the serogroups based on 11 L. monocytogenes isolated from farms was 72.7% and 27.3% for the serogroup 1/2a-3a and 4b-4d-4e, respectively, while for the 12 L. monocytogenes isolates recovered from abattoirs, it was 25%, 8.3%, 50% and 16.7% for the serogroup 1/2a-3a, 1/2b-3b, 1/2c-3c, and 4b-4d-4e respectively (P  < 0.05). All (100%) isolates of L. monocytogenes from the farms and abattoirs were positive for seven virulence genes ( hlyA , inlB , plcA , iap , inlA , inlC, and inlJ) . The clinical and food safety significance of the findings cannot be ignored.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0049-4747
1573-7438
DOI:10.1007/s11250-024-03934-y