Hydrogen Gas Inhalation Alleviates Airway Inflammation and Oxidative Stress on Ovalbumin-Induced Asthmatic BALB/c Mouse Model

Hydrogen Gas Inhalation Alleviates Airway Inflammation and Oxidative Stress on Ovalbumin-Induced Asthmatic BALB/c Mouse Model

Airway inflammatory diseases, such as asthma, are a global public health concern owing to their chronic inflammatory effects on the respiratory mucosa. Molecular hydrogen (H2) has recently been recognized for its antioxidant and anti-inflammatory properties. In this study, we examined the therapeuti...

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Bibliographic Details
Published in:Antioxidants Vol. 13; no. 11; p. 1328
Main Authors: He, Wenjing, Rahman, Md. Habibur, Bajgai, Johny, Abdul-Nasir, Sofian, Mo, Chaodeng, Ma, Hui, Goh, Seong Hoon, Bomi, Kim, Jung, Hyeran, Kim, Cheol-Su, Lee, Hyungdon, Lee, Kyu-Jae
Format: Journal Article
Language:English
Published: Basel MDPI AG 30-10-2024
MDPI
Subjects:
Anti-inflammatory agents
Antioxidants
Asthma
Blood
Colony-stimulating factor
Cytokines
Enzymatic activity
Enzymes
Gas detectors
Glutathione peroxidase
Granulocyte-macrophage colony-stimulating factor
Hydrogen
Hypersensitivity
Immunoglobulin E
Inflammation
Inflammatory diseases
Inhalation
Lymphocytes
Metastases
Neutrophils
Nitric oxide
Ovalbumin
Oxidative stress
Pathophysiology
Public health
Reactive oxygen species
Reagents
Respiratory tract diseases
Salbutamol
Tumor necrosis factor-TNF
Tumor necrosis factor-α
γ-Interferon
United States > US
South Korea
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Summary:Airway inflammatory diseases, such as asthma, are a global public health concern owing to their chronic inflammatory effects on the respiratory mucosa. Molecular hydrogen (H2) has recently been recognized for its antioxidant and anti-inflammatory properties. In this study, we examined the therapeutic potential of H2 in airway inflammation using an ovalbumin (OVA)-induced BALB/c mouse model of allergic asthma. Female BALB/c mice were sensitized and challenged with OVA to induce airway inflammation, and 30 mice were randomly divided into five groups: NT (non-treatment), HTC (3% H2 treatment only), NC (negative control, OVA only), PC (positive control, OVA + intranasal 1 mg/mL salbutamol 50 μL), and HT (H2 treatment, OVA + inhaled 3% H2). Various inflammatory and oxidative stress (OS)-induced markers such as white blood cells (WBCs) and their differential counts, lung histology, cytokine levels such as interleukin (IL)-4, (IL)-5, (IL)-13, interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), granulocyte-macrophage colony-stimulating factor (GM-CSF), (IL)-10, reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxidase (GPx), and catalase (CAT), and total immunoglobulin E (IgE) levels were investigated. Our results showed that inhaled H2 significantly reduced inflammatory cell infiltration, OS markers, and pro-inflammatory cytokine expression while upregulating antioxidant enzyme activity. Furthermore, H2 also significantly decreased serum IgE levels, a marker of allergic inflammation. Collectively, our findings suggest that H2 inhalation is a promising treatment option for airway inflammation, offering a novel approach with potential clinical applications.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox13111328