Search Results - "Miyazaki, Takatsugu"

Bacteroidota polysaccharide utilization system for branched dextran exopolysaccharides from lactic acid bacteria by Nakamura, Shuntaro, Kurata, Rikuya, Tonozuka, Takashi, Funane, Kazumi, Park, Enoch Y., Miyazaki, Takatsugu

“…Dextran is an α-(1→6)-glucan that is synthesized by some lactic acid bacteria, and branched dextran with α-(1→2)-, α-(1→3)-, and α-(1→4)-linkages are often…”
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Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis by Miyazaki, Takatsugu, Miyashita, Ryunosuke, Mori, Sota, Kato, Tatsuya, Park, Enoch Y.

“…N-glycans are involved in various physiological functions and their structures diverge among different phyla and kingdoms. Insect cells mainly produce high…”
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Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system by Miyazaki, Takatsugu, Kato, Tatsuya, Park, Enoch Y.

“…β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of…”
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Identification and Characterization of Dextran α-1,2-Debranching Enzyme from Microbacterium dextranolyticum by Miyazaki, Takatsugu, Tanaka, Hidekazu, Nakamura, Shuntaro, Dohra, Hideo, Funane, Kazumi

“…Dextran α-1,2-debranching enzyme (DDE) releases glucose with hydrolyzing α-(1→2)-glucosidic linkages in α-glucans, which are made up of dextran with…”
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N-Glycan Modification of a Recombinant Protein via Coexpression of Human Glycosyltransferases in Silkworm Pupae by Kato, Tatsuya, Kako, Natsumi, Kikuta, Kotaro, Miyazaki, Takatsugu, Kondo, Sachiko, Yagi, Hirokazu, Kato, Koichi, Park, Enoch Y.

“…Recombinant proteins produced in insect cells and insects, unlike those produced in mammalian cells, have pauci-mannose-type N -glycans. In this study, we…”
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A glycoside hydrolase family 31 dextranase with high transglucosylation activity from Flavobacterium johnsoniae by Gozu, Yoshifumi, Ishizaki, Yuichi, Hosoyama, Yuhei, Miyazaki, Takatsugu, Nishikawa, Atsushi, Tonozuka, Takashi

“…Glycoside hydrolase family (GH) 31 enzymes exhibit various substrate specificities, although the majority of members are α-glucosidases. Here, we constructed a…”
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The side chain of a glycosylated asparagine residue is important for the stability of isopullulanase by Miyazaki, Takatsugu, Yashiro, Hiroyuki, Nishikawa, Atsushi, Tonozuka, Takashi

“…N-glycosylation has been shown to be important for the stability of some glycoproteins. Isopullulanase (IPU), a polysaccharide-hydrolyzing enzyme, is a highly…”
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Combined Drug Resistance Mutations Substantially Enhance Enzyme Production in Paenibacillus agaridevorans by Funane, Kazumi, Tanaka, Yukinori, Hosaka, Takeshi, Murakami, Kiriko, Miyazaki, Takatsugu, Shiwa, Yuh, Gibu, Shigehachi, Inaoka, Takashi, Kasahara, Ken, Fujita, Nobuyuki, Yoshikawa, Hirofumi, Hiraga, Yoshikazu, Ochi, Kozo

“…This study shows that sequential introduction of drug resistance mutations substantially increased enzyme production in The triple mutant YT478 ( Gln225→stop…”
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Crystal structure and substrate-binding mode of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8 by Miyazaki, Takatsugu, Ichikawa, Megumi, Iino, Hitoshi, Nishikawa, Atsushi, Tonozuka, Takashi

“…Glycoside hydrolase family 63 (GH63) proteins are found in eukaryotes such as processing α-glucosidase I and also many bacteria and archaea. Recent studies…”
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Crystal Structure and Mutational Analysis of Isomalto-dextranase, a Member of Glycoside Hydrolase Family 27 by Okazawa, Yuka, Miyazaki, Takatsugu, Yokoi, Gaku, Ishizaki, Yuichi, Nishikawa, Atsushi, Tonozuka, Takashi

“…Arthrobacter globiformis T6 isomalto-dextranase (AgIMD) is an enzyme that liberates isomaltose from the non-reducing end of a polymer of glucose, dextran…”
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Comparison of the structural changes in two cellobiohydrolases, CcCel6A and CcCel6C, from Coprinopsis cinerea – a tweezer‐like motion in the structure of CcCel6C by Tamura, Mizuki, Miyazaki, Takatsugu, Tanaka, Yutaro, Yoshida, Makoto, Nishikawa, Atsushi, Tonozuka, Takashi

“…The basidiomycete Coprinopsis cinerea produces five cellobiohydrolases belonging to glycoside hydrolase family 6 (GH6). Among these enzymes, C. cinerea…”
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Crystal structure of the N-terminal domain of a glycoside hydrolase family 131 protein from Coprinopsis cinerea by Miyazaki, Takatsugu, Yoshida, Makoto, Tamura, Mizuki, Tanaka, Yutaro, Umezawa, Kiwamu, Nishikawa, Atsushi, Tonozuka, Takashi

“…•The structure of the N-terminal domain of a GH131 protein was determined.•The structure is composed of a β-jelly roll fold.•The possible active site is wide…”
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Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea by Liu, Yuan, Yoshida, Makoto, Kurakata, Yuma, Miyazaki, Takatsugu, Igarashi, Kiyohiko, Samejima, Masahiro, Fukuda, Kiyoharu, Nishikawa, Atsushi, Tonozuka, Takashi

“…The basidiomycete Coprinopsis cinerea produces the glycoside hydrolase family 6 enzyme CcCel6C at low and constitutive levels. CcCel6C exhibits unusual…”
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Glycoside hydrolases active on microbial exopolysaccharide α-glucans: structures and function by Miyazaki, Takatsugu

“…Glucose is the most abundant monosaccharide in nature and is an important energy source for living organisms. Glucose exists primarily as oligomers or polymers…”
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Structural insights into the inhibition mechanism of glucosidase inhibitors toward kojibiose hydrolase belonging to the glycoside hydrolase family 65 by Nakamura, Shuntaro, Miyazaki, Takatsugu

“…Glycoside hydrolase family 65 (GH65) includes glycoside hydrolases active on various α-glucosides. We previously demonstrated that the GH65 enzyme from…”
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Heterologous Expression, Purification, and Characterization of an α-Mannosidase Belonging to Glycoside Hydrolase Family 99 of Shewanella amazonensis by MATSUDA, Kana, KURAKATA, Yuma, MIYAZAKI, Takatsugu, MATSUO, Ichiro, ITO, Yukishige, NISHIKAWA, Atsushi, TONOZUKA, Takashi

“…Shewanella amazonensis α-mannosidase (Sama99), a member of glycoside hydrolase family 99, was expressed in Escherichia coli. The purified Sama99 hydrolyzed…”
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A Surface Loop in the N-Terminal Domain of Pedobacter heparinus Heparin Lyase II is Important for Activity by Mori, Marina, Ichikawa, Megumi, Kiguchi, Yumiko, Miyazaki, Takatsugu, Hattori, Makoto, Nishikawa, Atsushi, Tonozuka, Takashi

“…Pedobacter heparinus heparin lyase II (PhHepII) is composed of N-terminal, central, and C-terminal domains. A long surface loop, designated loop-A, is in the…”
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Structural insights into α-(1→6)-linkage preference of GH97 glucodextranase from Flavobacterium johnsoniae by Nakamura, Shuntaro, Kurata, Rikuya, Miyazaki, Takatsugu

“…Glycoside hydrolase family 97 (GH97) comprises enzymes like anomer-inverting α-glucoside hydrolases (i.e., glucoamylase) and anomer-retaining α-galactosidases…”
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Structural insights into α‐(1→6)‐linkage preference of GH 97 glucodextranase from Flavobacterium johnsoniae by Nakamura, Shuntaro, Kurata, Rikuya, Miyazaki, Takatsugu

“…Glycoside hydrolase family 97 (GH97) comprises enzymes like anomer‐inverting α‐glucoside hydrolases (i.e., glucoamylase) and anomer‐retaining α‐galactosidases…”
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G-quadruplex binding ability of TLS/FUS depends on the β-spiral structure of the RGG domain by Yagi, Ryota, Miyazaki, Takatsugu, Oyoshi, Takanori

“…Abstract The RGG domain, defined as closely spaced Arg-Gly-Gly repeats, is a DNA and RNA-binding domain in various nucleic acid-binding proteins. Translocated…”
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