Use of pcr-rflp of thefla a gene for detection and subtyping of Campylobacter jejuni strains Potentially related to Guillain-barré syndrome, isolated from humans and animals

The objectives of the present study were the subtyping of Campylobacter jejuni subsp. jejuni strains obtained from humans and different animal species using PCR-RFLP, and the detection, by means of the same technique, of strains related to serotype PEN O19:LIO 7, the main C. jejuni serotype linked t...

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Published in:	Brazilian journal of microbiology Vol. 40; no. 4; pp. 952 - 959
Main Authors:	Scarcelli, E.(Instituto Biológico Centro de Pesquisa e Desenvolvimento de Sanidade Animal), Piatti, R.M.(Instituto Biológico Centro de Pesquisa e Desenvolvimento de Sanidade Animal), Harakava, R.(Instituto Biológico Centro de Pesquisa e Desenvolvimento de Sanidade Animal), Miyashiro, S.(Instituto Biológico Centro de Pesquisa e Desenvolvimento de Sanidade Animal), Campos, F.R.(Instituto Biológico Centro de Pesquisa e Desenvolvimento de Sanidade Animal), Souza, M.C.A.(Agência Paulista de Tecnologia dos Agronegócios Pólo Regional do Vale do Paraíba Unidade de Pesquisa e Desenvolvimento de Pindamonhangaba), Cardoso, M.V.(Instituto Biológico Centro de Pesquisa e Desenvolvimento de Sanidade Animal), Teixeira, S.R.(Instituto Biológico Centro de Pesquisa e Desenvolvimento de Sanidade Animal), Genovez, M.E.(Instituto Biológico Centro de Pesquisa e Desenvolvimento de Sanidade Animal)
Format:	Journal Article
Language:	English
Published:	São Paulo Sociedade Brasileira de Microbiologia 01-12-2009 Springer Nature B.V
Subjects:	Bacteria Campylobacter jejuni Genes Guillain-Barré Syndrome MICROBIOLOGY Neurological disorders Pathology PCR-RFLP Polymerase chain reaction subtyping Guillain-Barré Syndrome PCR-RFLP subtyping Campylobacter jejuni
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Summary:	The objectives of the present study were the subtyping of Campylobacter jejuni subsp. jejuni strains obtained from humans and different animal species using PCR-RFLP, and the detection, by means of the same technique, of strains related to serotype PEN O19:LIO 7, the main C. jejuni serotype linked to Guillain-Barré Syndrome (GBS). Seventy C. jejuni strains isolated from human feces (n=33), primates (n=15), dogs (n=5), swine (n=2), bovines (n=1), abortion material from goats (n=2) and poultry carcasses (n=12), all collected in the state of São Paulo, were subtyped by means of PCR-RFLP of fla A gene, using restriction endonucleases Hae III, Afa I and Mbo I. Seven subtypes were observed when using the enzyme Hae III; eight when using Mbo I; and seven when using Afa I. The combination of the three endonucleases led to 16 fla-RFLP subtypes, from which ten subtypes shared strains of human and animal origin. From these, seven subtypes were observed in human and broiler strains. In eight subtypes, the other animal species shared patterns with human strains. It was inferred that, besides broilers, swine, goats, dogs and primates may be sources of infection for human in São Paulo. PCR-RFLP is a highly discriminatory technique that may be applied to molecular epidemiology studies of samples from different origins. Besides, the study also enabled the detection of two human strains and two primate strains related to serotype PEN O19: LIO 7.
Bibliography:	10.1590/S1517-83822009000400029 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400029
ISSN:	1517-8382 1678-4405 1678-4405
DOI:	10.1590/S1517-83822009000400029