Lectin histochemistry in rat liver fibrosis induced by heterologous serum sensitization

The localization of carbohydrates in rat livers with fibrosis induced by heterologous serum was examined by lectin histochemical and biochemical techniques. Twenty-four lectins were used to visualize the different carbohydrates in paraffin sections of normal and fibrotic liver tissues. No difference...

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Bibliographic Details
Published in:	Journal of Veterinary Medical Science Vol. 59; no. 8; pp. 681 - 687
Main Authors:	Miyamaoto, M. (Kyoto Univ. (Japan)), Manabe, N, Kuramitsu, K, Kuribayashi, Y, Tamura, K, Furuya, Y, Nagano, N, Fukumoto, M, Miyamoto, H
Format:	Journal Article
Language:	English
Published:	Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01-08-1997 Japan Science and Technology Agency
Subjects:	ANALISIS HISTOCITOLOGICO ANALYSE HISTOCYTOLOGIQUE Animals BLOOD SERUM Blood Transfusion CARBOHIDRATOS carbohydrate localization CARBOHYDRATES ENFERMEDADES HEPATICAS GLUCIDE Glycoconjugates - analysis hepatic fibrosis HISTOCYTOLOGICAL ANALYSIS IMMUNISATION IMMUNIZATION INMUNIZACION lectin histochemistry LECTINAS LECTINE LECTINS Liver - cytology Liver - pathology Liver Cirrhosis, Experimental - pathology LIVER DISEASES MALADIE DU FOIE Male RAT RATA RATS Rats, Sprague-Dawley Reference Values SERUM SANGUIN serum sensitization SUERO SANGUINEO Swine Transplantation, Heterologous
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Summary:	The localization of carbohydrates in rat livers with fibrosis induced by heterologous serum was examined by lectin histochemical and biochemical techniques. Twenty-four lectins were used to visualize the different carbohydrates in paraffin sections of normal and fibrotic liver tissues. No differences in staining patterns of these lectins were observed between normal and fibrotic livers in hepatocyte cell membranes including bile canaliculi, sinusoidal endothelial, or bile ductal cells. Kupffer cells strongly stained with Vicia villosa agglutinin (VVA) were seen only in the periportal zone of the normal liver, but they were observed in the periportal zone and scattered throughout the pseudolobular zone in the fibrotic liver. The cytoplasm of some hepatocytes was strongly stained by Bandeiraea simplicifolia lectin-I (BSL-I). BSL-I positive hepatocytes in normal liver were localized in the periportal zone, but those in the fibrotic liver were scattered in the periportal and perifibrous zones. After polyacrylamide gel electrophoresis of liver glycoproteins, differences in molecular sizes of BSL-I positive glycoproteins (79 and 81 kD) were detected by lectin blotting. Cell density of perifibrous BSL-I positive hepatocytes may be useful as a diagnostic parameter for liver fibrosis and/or cirrhosis. Two distinct staining patterns with twelve lectins were observed in fibrotic septa of the fibrotic liver. The fibrotic septa were stained with six characteristic lectins, and the centrilobular septa were stained with all these twelve of lectins. Histopathological assessment of the centrilobular fibrotic septa stained with these characteristic lectins may contribute to the diagnosis and prognosis of hepatic fibrosis.
Bibliography:	L73 1997006864 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0916-7250 1347-7439
DOI:	10.1292/jvms.59.681