Identification of the Three Genes Involved in Controlling Production of a Phytotoxin Tropolone in Burkholderia plantarii

Tropolone, a phytotoxin produced by Burkholderia plantarii, causes rice seedling blight. To identify genes involved in tropolone synthesis, we systematically constructed mutations in the genes encoding 55 histidine kinases and 72 response regulators. From the resulting defective strains, we isolated...

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Published in:	Journal of bacteriology Vol. 198; no. 11; pp. 1604 - 1609
Main Authors:	Miwa, Shunpei, Kihira, Eri, Yoshioka, Akinori, Nakasone, Kaoru, Okamoto, Sho, Hatano, Masaki, Igarashi, Masayuki, Eguchi, Yoko, Kato, Akinori, Ichikawa, Natsuko, Sekine, Mitsuo, Fujita, Nobuyuki, Kanesaki, Yu, Yoshikawa, Hirofumi, Utsumi, Ryutaro
Format:	Journal Article
Language:	English
Published:	United States American Society for Microbiology 01-06-2016
Subjects:	Bacteriology Burkholderia - genetics Burkholderia - metabolism Burkholderia plantarii Gene Expression Regulation, Bacterial - physiology Genes Gram-negative bacteria Molecular Structure Oryza - microbiology Plant Diseases - microbiology Toxins Tropolone - chemistry Tropolone - metabolism
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Summary:	Tropolone, a phytotoxin produced by Burkholderia plantarii, causes rice seedling blight. To identify genes involved in tropolone synthesis, we systematically constructed mutations in the genes encoding 55 histidine kinases and 72 response regulators. From the resulting defective strains, we isolated three mutants, KE1, KE2, and KE3, in which tropolone production was repressed. The deleted genes of these mutants were named troR1, troK, and troR2, respectively. The mutant strains did not cause rice seedling blight, and complementation experiments indicated that TroR1, TroK, and TroR2 were involved in the synthesis of tropolone in B. plantarii However, tropolone synthesis was repressed in the TroR1 D52A, TroK H253A, and TroR2 D46A site-directed mutants. These results suggest that the putative sensor kinase (TroK) and two response regulators (TroR1 and TroR2) control the production of tropolone in B. plantarii A two-component system is normally composed of a sensor histidine kinase (HK) and a cognate response regulator (RR) pair. In this study, HK (TroK) and two RRs (TroR1 and TroR2) were found to be involved in controlling tropolone production in B. plantarii These three genes may be part of a bacterial signal transduction network. Such networks are thought to exist in other bacteria to regulate phytotoxin production, as well as environmental adaptation and signal transduction.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Citation Miwa S, Kihira E, Yoshioka A, Nakasone K, Okamoto S, Hatano M, Igarashi M, Eguchi Y, Kato A, Ichikawa N, Sekine M, Fujita N, Kanesaki Y, Yoshikawa H, Utsumi R. 2016. Identification of the three genes involved in controlling production of a phytotoxin tropolone in Burkholderia plantarii. J Bacteriol 198:1604–1609. doi:10.1128/JB.01028-15. S.M., E.K., A.Y., and K.N. contributed equally to this work.
ISSN:	0021-9193 1098-5530
DOI:	10.1128/jb.01028-15