Search Results - "Mittl, Peer R.E"

Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase by Obexer, Richard, Godina, Alexei, Garrabou, Xavier, Mittl, Peer R. E., Baker, David, Griffiths, Andrew D., Hilvert, Donald

“…Designing catalysts that achieve the rates and selectivities of natural enzymes is a long-standing goal in protein chemistry. Here, we show that an…”
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Noncanonical Heme Ligands Steer Carbene Transfer Reactivity in an Artificial Metalloenzyme by Pott, Moritz, Tinzl, Matthias, Hayashi, Takahiro, Ota, Yusuke, Dunkelmann, Daniel, Mittl, Peer R. E., Hilvert, Donald

“…Changing the primary metal coordination sphere is a powerful strategy for tuning metalloprotein properties. Here we used amber stop codon suppression with…”
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Evolution of a highly active and enantiospecific metalloenzyme from short peptides by Studer, Sabine, Hansen, Douglas A, Pianowski, Zbigniew L, Mittl, Peer R E, Debon, Aaron, Guffy, Sharon L, Der, Bryan S, Kuhlman, Brian, Hilvert, Donald

“…Primordial sequence signatures in modern proteins imply ancestral origins tracing back to simple peptides. Although short peptides seldom adopt unique folds,…”
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Emergence of a Negative Activation Heat Capacity during Evolution of a Designed Enzyme by Bunzel, H. Adrian, Kries, Hajo, Marchetti, Luca, Zeymer, Cathleen, Mittl, Peer R. E, Mulholland, Adrian J, Hilvert, Donald

“…Temperature influences the reaction kinetics and evolvability of all enzymes. To understand how evolution shapes the thermodynamic drivers of catalysis, we…”
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Precision is essential for efficient catalysis in an evolved Kemp eliminase by Blomberg, Rebecca, Kries, Hajo, Pinkas, Daniel M., Mittl, Peer R. E., Grütter, Markus G., Privett, Heidi K., Mayo, Stephen L., Hilvert, Donald

“…A computationally designed enzyme that was evolved to accelerate a chemical reaction 6 × 10 8 -fold approaches the exceptional efficiency of highly optimized…”
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A Chemically Programmed Proximal Ligand Enhances the Catalytic Properties of a Heme Enzyme by Green, Anthony P, Hayashi, Takahiro, Mittl, Peer R. E, Hilvert, Donald

“…Enzymes rely on complex interactions between precisely positioned active site residues as a mechanism to compensate for the limited functionality contained…”
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Drug Design Inspired by Nature: Crystallographic Detection of an Auto‐Tailored Protease Inhibitor Template by Gall, Flavio M., Hohl, Deborah, Frasson, David, Wermelinger, Tobias, Mittl, Peer R. E., Sievers, Martin, Riedl, Rainer

“…De novo drug discovery is still a challenge in the search for potent and selective modulators of therapeutically relevant target proteins. Here, we disclose…”
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Computationally Designed Armadillo Repeat Proteins for Modular Peptide Recognition by Reichen, Christian, Hansen, Simon, Forzani, Cristina, Honegger, Annemarie, Fleishman, Sarel J., Zhou, Ting, Parmeggiani, Fabio, Ernst, Patrick, Madhurantakam, Chaithanya, Ewald, Christina, Mittl, Peer R.E., Zerbe, Oliver, Baker, David, Caflisch, Amedeo, Plückthun, Andreas

“…Armadillo repeat proteins (ArmRPs) recognize their target peptide in extended conformation and bind, in a first approximation, two residues per repeat. Thus,…”
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Structure‐based optimization of designed Armadillo‐repeat proteins by Madhurantakam, Chaithanya, Varadamsetty, Gautham, Grütter, Markus G., Plückthun, Andreas, Mittl, Peer R.E.

“…The armadillo domain is a right‐handed super‐helix of repeating units composed of three α‐helices each. Armadillo repeat proteins (ArmRPs) are frequently…”
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Specific Inhibition of Caspase-3 by a Competitive DARPin: Molecular Mimicry between Native and Designed Inhibitors by Schroeder, Thilo, Barandun, Jonas, Flütsch, Andreas, Briand, Christophe, Mittl, Peer R.E., Grütter, Markus G.

“…Dysregulation of apoptosis is associated with several human diseases. The main apoptotic mediators are caspases, which propagate death signals to downstream…”
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Structure-Activity Studies in a Family of β-Hairpin Protein Epitope Mimetic Inhibitors of the p53-HDM2 Protein-Protein Interaction by Fasan, Rudi, Dias, Ricardo L.A, Moehle, Kerstin, Zerbe, Oliver, Obrecht, Daniel, Mittl, Peer R.E, Grütter, Markus G, Robinson, John A

“…Inhibitors of the interaction between the p53 tumor-suppressor protein and its natural human inhibitor HDM2 are attractive as potential anticancer agents. In…”
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Stabilizing Ionic Interactions in a Full-consensus Ankyrin Repeat Protein by Merz, Tobias, Wetzel, Svava K., Firbank, Susan, Plückthun, Andreas, Grütter, Markus G., Mittl, Peer R.E.

“…Full-consensus designed ankyrin repeat proteins (DARPins), in which randomized positions of the previously described DARPin library have been fixed, are…”
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Autoproteolytic and Catalytic Mechanisms for the β-Aminopeptidase BapA—A Member of the Ntn Hydrolase Family by Merz, Tobias, Heck, Tobias, Geueke, Birgit, Mittl, Peer R.E., Briand, Christophe, Seebach, Dieter, Kohler, Hans-Peter E., Grütter, Markus G.

“…The β-aminopeptidase BapA from Sphingosinicella xenopeptidilytica belongs to the N-terminal nucleophile (Ntn) hydrolases of the DmpA-like family and has the…”
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Structure of the PRYSPRY-domain: Implications for autoinflammatory diseases by Grütter, Christian, Briand, Christophe, Capitani, Guido, Mittl, Peer R.E., Papin, Stephanie, Tschopp, Jürg, Grütter, Markus G.

“…We determined the first structure of PRYSPRY, a domain found in over 500 different proteins, involved in innate immune signaling, cytokine signaling…”
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Extended Substrate Recognition in Caspase-3 Revealed by High Resolution X-ray Structure Analysis by Ganesan, Rajkumar, Mittl, Peer R.E., Jelakovic, Stjepan, Grütter, Markus G.

“…Caspases are cysteine proteases involved in the signalling cascades of programmed cell death in which caspase-3 plays a central role, since it propagates death…”
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The crystal structure of Helicobacter cysteine-rich protein C at 2.0 A resolution: similar peptide-binding sites in TPR and SEL1-like repeat proteins by Lüthy, Lucas, Grütter, Markus G, Mittl, Peer R E

“…Helicobacter pylori is a Gram-negative human pathogen that infects the gastric mucosa and causes an inflammatory process leading to gastritis, ulceration and…”
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Helicobacter pylori evolution: lineage- specific adaptations in homologs of eukaryotic Sel1-like genes by Ogura, Masako, Perez, J Christian, Mittl, Peer R E, Lee, Hae-Kyung, Dailide, Geidrius, Tan, Shumin, Ito, Yoshiyuki, Secka, Ousman, Dailidiene, Daiva, Putty, Kalyani, Berg, Douglas E, Kalia, Awdhesh

“…Geographic partitioning is postulated to foster divergence of Helicobacter pylori populations as an adaptive response to local differences in predominant host…”
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Structural genomics: opportunities and challenges by Mittl, Peer R.E, Grütter, Markus G

“…Following the complete genome sequencing of an increasing number of organisms, structural biology is engaging in a systematic approach of high-throughput…”
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The Crystal Structure of Metal-free Human EF-hand Protein S100A3 at 1.7-Å Resolution by Fritz, Gu¨nter, Mittl, Peer R.E., Vasak, Milan, Gru¨tter, Markus G., Heizmann, Claus W.

“…S100A3 is a unique member of the EF-hand superfamily of Ca2+-binding proteins. It binds Ca2+ with poor affinity (Kd = 4–35 mm) but Zn2+ with exceptionally high…”
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A Noncanonical Proximal Heme Ligand Affords an Efficient Peroxidase in a Globin Fold by Pott, Moritz, Hayashi, Takahiro, Mori, Takahiro, Mittl, Peer R. E., Green, Anthony P., Hilvert, Donald

“…Expanding the range of genetically encoded metal coordination environments accessible within tunable protein scaffolds presents excellent opportunities for the…”
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