Derivation of human embryonic stem cells in defined conditions

We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that i...

Full description

Saved in:

Bibliographic Details
Published in:	Nature biotechnology Vol. 24; no. 2; pp. 185 - 187
Main Authors:	Thomson, James A, Ludwig, Tenneille E, Levenstein, Mark E, Jones, Jeffrey M, Berggren, W Travis, Mitchen, Erika R, Frane, Jennifer L, Crandall, Leann J, Daigh, Christine A, Conard, Kevin R, Piekarczyk, Marian S, Llanas, Rachel A
Format:	Journal Article
Language:	English
Published:	New York, NY Nature 01-02-2006 Nature Publishing Group
Subjects:	Animal cells Biological and medical sciences Biotechnology Cell Culture Techniques - methods Cell Differentiation Cell Line Cell Proliferation Cell Survival Culture Media - chemistry Culture Media - metabolism Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Genetic recombination Humans Methods. Procedures. Technologies Proteins Stem cells Stem Cells - cytology Stem Cells - physiology Tissue Engineering - methods Human Cell culture Embryo Cell line Stem cell Protein Growth factor
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1087-0156 1546-1696
DOI:	10.1038/nbt1177