Search Results - "Misoka, Fusakazu"
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Protease II from Escherichia coli: sequencing and expression of the enzyme gene and characterization of the expressed enzyme
Published in Journal of biochemistry (Tokyo) (1991)“…Protease II gene of Escherichia coli HB101 was cloned and expressed in E. coli JM83. The transformant harboring a hybrid plasmid, pPROII-12, with a 2.4 kbp…”
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Overproduction of human insulin-like growth factor-II inEscherichia coli
Published in Biotechnology letters (01-12-1989)Get full text
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Secretion of human interferon-alpha induced by using secretion vectors containing a promoter and signal sequence of alkaline phosphatase gene of Escherichia coli
Published in Journal of biochemistry (Tokyo) (01-05-1985)“…We constructed a new vector containing the promoter and the signal sequence of E. coli phoA gene, the structural gene for the periplasmic alkaline phosphatase…”
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Efficient purification and analysis for stability of human secretin precursor-like peptide produced inEscherichia coli
Published in Biotechnology letters (01-08-1991)Get full text
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Efficient purification and analysis for stability of human secretin precursor-like peptide produced in Escherichia coli
Published in Biotechnology letters (01-08-1991)“…A gene coding for human secretin elongated at the C-terminus by Gly-Lys-Arg, designated as SecGKR gene, was fused to downstream of an artificial human growth…”
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Production in Escherichia coli of Biologically Active Secretin, a Gastrointestinal Hormone
Published in Proceedings of the National Academy of Sciences - PNAS (01-04-1982)“…A synthetic gene for porcine secretin was ligated with Pst I-cleaved pBR322 by its flanking synthetic Pst I linkers to produce a fused protein consisting of an…”
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