Influence of natural polysaccharides on the morphology and properties of hybrid vaterite microcrystals

Influence of natural polysaccharides on the morphology and properties of hybrid vaterite microcrystals

Co-precipitation of biopolymers into calcium carbonate crystals changes their physicochemical and biological properties. This work studies hybrid microcrystals of vaterite obtained in the presence of natural polysaccharides, as carriers for the delivery of proteins and enzymes. Hybrid microcrystals...

Full description

Saved in:
Bibliographic Details
Published in:Heliyon Vol. 10; no. 13; p. e33801
Main Authors: Balabushevich, Nadezhda G., Maltseva, Liliya N., Filatova, Lyubov Y., Mosievich, Daniil V., Mishin, Pavel I., Bogomiakova, Margarita E., Lebedeva, Olga S., Murina, Marina A., Klinov, Dmitry V., Obraztsova, Ekaterina A., Kharaeva, Zaira F., Firova, Roxalana K., Grigorieva, Daria V., Gorudko, Irina V., Panasenko, Oleg M., Mikhalchik, Elena V.
Format: Journal Article
Language:English
Published: England Elsevier Ltd 15-07-2024
Elsevier
Subjects:
Antioxidant properties
Biocompatibility
Calcium carbonate (vaterite)
Drug delivery
Enzyme activity
Protein adsorption
CCHE
Km
Enzyme activity
Drug delivery
IBC
Calcium carbonate (vaterite)
ADP
PRP
CCCS
heparin
S
qe
Dpores
dextran sulfate
pectin
Biocompatibility
vmax
Antioxidant properties
polysaccharides
chondroitin sulfate
Protein adsorption
fucoidan
CCDS
CCFU
BET
APTT
Dcrystal
LCL
Dnanocrystalline
Vpores
and CCPE
kcat
microcrystals of vaterite (calcium carbonate)
SEM
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Co-precipitation of biopolymers into calcium carbonate crystals changes their physicochemical and biological properties. This work studies hybrid microcrystals of vaterite obtained in the presence of natural polysaccharides, as carriers for the delivery of proteins and enzymes. Hybrid microcrystals with dextran sulfate, chondroitin sulfate, heparin, fucoidan, and pectin were obtained and compared. The impact of polysaccharides on the morphology (particle diameter, surface area, nanocrystallite and pore size), polysaccharide content and surface charge of hybrid microcrystals was studied. Only microcrystals with fucoidan and heparin exhibited antioxidant activity against •ОН radical. The surface charge and pore size of the hybrid microcrystals affected the sorption of albumin, catalase, chymotrypsin, mucin. A decrease in the catalytic constant and Michaelis constant was observed for catalase sorbed on the hybrid crystals. The biocompatibility of microcrystals depended on the nature of the included polysaccharide: crystals with sulfated polysaccharides increased blood plasma coagulation but not platelet aggregation, and crystals with dextran sulfate had the greatest cytotoxicity against HT-29 cells but not erythrocytes. Hybrid microcrystals with all polysaccharides except chondroitin sulfate reduced erythrocyte lysis in vitro compared with vaterite crystals. The obtained results enable to create novel carriers based on hybrid vaterite crystals with polysaccharides, beneficial for the delivery of protein drugs. [Display omitted] •Hybrid microcrystals of vaterite with natural polysaccharides were obtained by spontaneous crystallization.•Diameter, morphology, charge, antioxidant properties of hybrid microcrystals depended on the nature of polysaccharides.•The surface charge and pore size of the hybrid microcrystals influenced sorption of mucin and proteins and enzyme activity.•Microcrystals differed in the effects on plasma coagulation, platelet aggregation, erythrocytes lysis and HT-29 cells.•The use of different polysaccharides improves the properties of hybrid microcrystals of vaterite, incorporation of target proteins and biological activity.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2024.e33801