Spinal Cord Injury Affects Gene Expression of Transmembrane Proteins in Tissue and Release of Extracellular Vesicle in Blood: In Silico and In Vivo Analysis

Spinal Cord Injury Affects Gene Expression of Transmembrane Proteins in Tissue and Release of Extracellular Vesicle in Blood: In Silico and In Vivo Analysis

Spinal cord injury (SCI) can disrupt membrane transmission by affecting transmembrane channels or neurotransmitter release. This study aimed to explore gene expression changes of transmembrane proteins underlying SCI through bioinformatics approaches and confirming in SCI model in rats. In this expe...

Full description

Saved in:
Bibliographic Details
Published in:Cell journal (Yakhteh) Vol. 25; no. 11; pp. 772 - 782
Main Authors: Mirzaalikhan, Yasmin, Eslami, Nasim, Izadi, Amin, Shekari, Faezeh, Kiani, Sahar
Format: Journal Article
Language:English
Published: Iran Royan Institute of Iran 01-11-2023
Royan Institute
Royan Institute (ACECR), Tehran
Subjects:
Annotations
Bioinformatics
Blood
CD63 antigen
CD9 antigen
Centrifugation
CXCR4 protein
differentially expressed genes
DNA microarrays
extracellular vesicles
Gene expression
Genes
Helices
Injury analysis
membrane protein
Membranes
Neurotransmitter release
Original
Polymerase chain reaction
Protein expression
Proteins
Ribonucleic acid
RNA
signaling pathways
Spinal cord injuries
spinal cord injury
Transmembrane proteins
Western blotting
Membrane Protein
Differentially Expressed Genes
Signaling Pathways
Extracellular Vesicles
Spinal Cord Injury
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Spinal cord injury (SCI) can disrupt membrane transmission by affecting transmembrane channels or neurotransmitter release. This study aimed to explore gene expression changes of transmembrane proteins underlying SCI through bioinformatics approaches and confirming in SCI model in rats. In this experimental study, the differentially expressed genes (DEGs) in acute and subacute SCI were obtained based on microarray data downloaded from the gene expression omnibus (GEO). Transmembrane proteins of DEGs were recognized by using the UniProt annotation and transmembrane helices prediction (TMHMM) methods. The model of SCI was established through a weight-dropping procedure in rats. To confirm the SCI model, hematoxylin and eosin (H and E) staining was performed. Total mRNA was extracted from spinal cord tissues, and the RNA expression profile of some of the significantly changed genes in the previous part that has been confirmed by real-time polymerase chain reaction (PCR). Blood was collected from rats before sacrificing. Extracellular vesicles (EVs) were isolated by high-speed centrifugation from plasma. For the assessment of protein expression, western blotting was used. Based on bioinformatics analysis, we candidated a set of membrane proteins in SCI's acute and sub-acute phases, and confirmed significant upregulation in and between the acute and control groups and downregulation in Enpp3 between acute and subacute groups at the RNA level. Considering CD63 as an EV marker, we examined the protein expression of and in the plasma-derived EVs, and CD9 has significant expression between acute and control groups. We also demonstrate no significant and expressions between groups. Our results provide new insight into the relationship between candidate transmembrane protein expression and different stages of SCI using in-silico approaches. Also, results show the release of EVs in blood in each group after SCI helping enlarge strategies to enhance recovery following SCI.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2228-5806
2228-5814
DOI:10.22074/CELLJ.2023.2004115.1320