Search Results - "Miret, Joan"

Enabling HEK293 cells for antibiotic-free media bioprocessing through CRISPR/Cas9 gene editing by Román, Ramón, Miret, Joan, Roura, Aïda, Casablancas, Antoni, Lecina, Martí, Cairó, Jordi J.

“…[Display omitted] •We developed 2 new metabolic selectable markers for protein production in HEK293.•We developed gene-edited cell lines in order to test TYMS…”
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Homogeneous antibody-drug conjugates: DAR 2 anti-HER2 obtained by conjugation on isolated light chain followed by mAb assembly by Farràs, Mercè, Miret, Joan, Camps, Marc, Román, Ramón, Martínez, Óscar, Pujol, Xavier, Erb, Stéphane, Ehkirch, Anthony, Cianferani, Sarah, Casablancas, Antoni, Cairó, Jordi Joan

“…Despite advances in medical care, cancer remains a major threat to human health. Antibody-drug conjugates (ADCs) are a promising targeted therapy to overcome…”
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Heavy chain dimers stabilized by disulfide bonds are required to promote in vitro assembly of trastuzumab by Farràs, Mercè, Román, Ramón, Camps, Marc, Miret, Joan, Martínez, Óscar, Pujol, Xavier, Casablancas, Antoni, Cairó, Jordi Joan

“…Monoclonal antibodies (mAbs) and their derivatives have become one of the most important classes of therapeutic drugs. Their multiple applications increased…”
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Enhancing heterologous protein expression and secretion in HEK293 cells by means of combination of CMV promoter and IFNα2 signal peptide by Román, Ramón, Miret, Joan, Scalia, Federica, Casablancas, Antoni, Lecina, Martí, Cairó, Jordi J.

“…•A screening for best promoters and signal peptides for recombinant protein production in HEK293 cell line was performed.•IFNα2 signal peptide was used for the…”
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Enhancing heterologous protein expression and secretion in HEK293 cells by means of combination of CMV promoter and IFN alpha 2 signal peptide by Roman, Ramon, Miret, Joan, Scalia, Federica, Casablancas, Antoni, Lecina, Marti, Cairo, Jordi J

“…Efficient production and secretion of recombinant proteins in mammalian cell lines relies in a combination of genetic, metabolic and culture strategy factors…”
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Development of a highly efficient production process for recombinant protein expression in Escherichia coli NEB10β by Miret, Joan, Román, Ramón, Benito, Mario, Casablancas, Antoni, Guillén, Marina, Álvaro, Gregorio, González, Glòria

“…•Molecular biology strain E. coli NEB10β produces high product titers in bioreactor.•High productivity using fed-batch process using glycerol as a carbon…”
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Metabolic flux balance analysis during lactate and glucose concomitant consumption in HEK293 cell cultures by Martínez‐Monge, Iván, Albiol, Joan, Lecina, Martí, Liste‐Calleja, Leticia, Miret, Joan, Solà, Carles, Cairó, Jordi J.

“…At early stages of the exponential growth phase in HEK293 cell cultures, the tricarboxylic acid cycle is unable to process all the amount of NADH generated in…”
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Study on vector elements for efficient selection, expression and secretion in HEK293 cells by Roman, Ramon, Miret, Joan, Martinez, Ivan, Roura, Aïda, Casablancas, Antoni, Lecina, Martí, Cairó, Jordi J.

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Insight on the Impact of the Reduction Step on the Site‐Directed Conjugation of an Anti‐HER2 Cysteine‐Engineered Antibody by Miret, Joan, Camps, Marc, Farràs, Mercè, Román, Ramón, Erb, Stéphane, Cianferani, Sarah, Casablancas, Antoni, Cairó, Jordi J.

“…Cysteine‐based conjugation is one of the main antibody conjugation strategies for generating both heterogeneous and homogeneous Antibody Drug Conjugates…”
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Homogeneous antibody-drug conjugates: DAR 2 anti-HER2 obtained by conjugation on isolated light chain followed by mAb assembly by Farràs, Mercè, Miret, Joan, Camps, Marc, Román, Ramón, Martínez, Óscar, Pujol, Xavier, Erb, Stéphane, Ehkirch, Anthony, Cianferani, Sarah, Casablancas, Antoni, Cairó, Jordi Joan

“…Despite advances in medical care, cancer remains a major threat to human health. Antibody-drug conjugates (ADCs) are a promising targeted therapy to overcome…”
Get full text