Search Results - "Miranda, G. S."

Mechanisms of Protein Stabilization and Prevention of Protein Aggregation by Glycerol by Vagenende, Vincent, Yap, Miranda G. S, Trout, Bernhardt L

“…The stability of proteins in aqueous solution is routinely enhanced by cosolvents such as glycerol. Glycerol is known to shift the native protein ensemble to…”
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study of monoclonal antibody-producing CHO cell lines: What makes a stable high producer? by Chusainow, Janet, Yang, Yuan Sheng, Yeo, Jessna H.M, Toh, Poh Choo, Asvadi, Parisa, Wong, Niki S.C, Yap, Miranda G.S

“…Generating stable, high-producing cell lines for recombinant protein production requires an understanding of the potential limitations in the cellular…”
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Animal performance and meat quality characteristics from feedlot-finished steers fed increasing levels of wet distillers grain by Pouzo, L.B., Ceconi, I., Davies, P., Méndez, D., Ortiz Miranda, S.G., Testa, M.L., Pavan, E.

“…One hundred forty-four steers were group-housed in 24 pens that were randomly assigned to one of four dietary treatments defined by the proportion of wet…”
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DNA methylation contributes to loss in productivity of monoclonal antibody-producing CHO cell lines by Yang, Yuansheng, Mariati, Chusainow, Janet, Yap, Miranda G.S.

“…Production instability currently limits the use of mammalian cells for industrial production of therapeutic proteins. We have previously reported that the loss…”
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IRES-mediated Tricistronic vectors for enhancing generation of high monoclonal antibody expressing CHO cell lines by Ho, Steven C.L., Bardor, Muriel, Feng, Huatao, Mariati, Tong, Yen Wah, Song, Zhiwei, Yap, Miranda G.S., Yang, Yuansheng

“…A Tricistronic vector utilizing internal ribosome entry site (IRES) elements to express the light chain (LC), heavy chain (HC), and a neomycin…”
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Combined in silico modeling and metabolomics analysis to characterize fed-batch CHO cell culture by Selvarasu, Suresh, Ho, Ying Swan, Chong, William P. K., Wong, Niki S. C., Yusufi, Faraaz N. K., Lee, Yih Yean, Yap, Miranda G. S., Lee, Dong-Yup

“…The increasing demand for recombinant therapeutic proteins highlights the need to constantly improve the efficiency and yield of these biopharmaceutical…”
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investigation of intracellular glycosylation activities in CHO cells: Effects of nucleotide sugar precursor feeding by Wong, Niki S.C, Wati, Lydia, Nissom, Peter M, Feng, H.T, Lee, M.M, Yap, Miranda G.S

“…Controlling glycosylation of recombinant proteins produced by CHO cells is highly desired as it can be directed towards maintaining or increasing product…”
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Engineering mammalian cells in bioprocessing - current achievements and future perspectives by Lim, Yiping, Wong, Niki S. C., Lee, Yih Yean, Ku, Sebastian C. Y., Wong, Danny C. F., Yap, Miranda G. S.

“…Over the past 20 years, we have seen significant improvements in product titres from 50 mg/l to 5–10 g/l, a more than 100‐fold increase. The main methods that…”
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Uncovering genes with divergent mRNA-protein dynamics in Streptomyces coelicolor by Jayapal, Karthik P, Philp, Robin J, Kok, Yee-Jiun, Yap, Miranda G S, Sherman, David H, Griffin, Timothy J, Hu, Wei-Shou

“…Many biological processes are intrinsically dynamic, incurring profound changes at both molecular and physiological levels. Systems analyses of such processes…”
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Heat shock protein 27 overexpression in CHO cells modulates apoptosis pathways and delays activation of caspases to improve recombinant monoclonal antibody titre in fed-batch bioreactors by Tan, Janice G.L., Lee, Yih Yean, Wang, Tianhua, Yap, Miranda G. S., Tan, Tin Wee, Ng, Say Kong

“…CHO cells are major production hosts for recombinant biologics including the rapidly expanding recombinant monoclonal antibodies (mAbs). Heat shock protein 27…”
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Molecular Anatomy of Preferential Interaction Coefficients by Elucidating Protein Solvation in Mixed Solvents: Methodology and Application for Lysozyme in Aqueous Glycerol by Vagenende, Vincent, Yap, Miranda G. S, Trout, Bernhardt L

“…Preferential interaction coefficients of proteins in mixed solvents are bulk thermodynamic parameters that relate molecular characteristics of protein…”
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Multitagging Proteomic Strategy to Estimate Protein Turnover Rates in Dynamic Systems by Jayapal, Karthik P, Sui, Siguang, Philp, Robin J, Kok, Yee-Jiun, Yap, Miranda G. S, Griffin, Timothy J, Hu, Wei-Shou

“…Current techniques for quantitative proteomics focus mainly on measuring overall protein dynamics, which is the net result of protein synthesis and…”
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Effects of overexpression of X-box binding protein 1 on recombinant protein production in Chinese hamster ovary and NS0 myeloma cells by Ku, Sebastian C.Y, Ng, Daphne T.W, Yap, Miranda G.S, Chao, Sheng-Hao

“…X-box binding protein 1 (XBP-1) is a key regulator of the cellular secretory pathway and unfolded protein response (UPR). It has been shown that the spliced…”
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Selenomethionine increases proliferation and reduces apoptosis in bovine mammary epithelial cells under oxidative stress by Miranda, S G, Purdie, N G, Osborne, V R, Coomber, B L, Cant, J P

“…The decline in mammary epithelial cell number as lactation progresses may be due, in part, to oxidative stress. Selenium is an integral component of several…”
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The schistosomicidal activity of ethanolic extracts from branches, leaves, flowers and fruits of Handroanthus impetiginosus (Mart. ex DC.) Mattos (Bignoniaceae) plant and metabolic profile characterization by UPLC-ESI-QTOF analysis by Montagnini, D. L., Katchborian-Neto, A., Tahan, M. P. M., Oliveira, N. D., Magalhães, L. G., Januário, A. H., Pauletti, P. M., Cavallari, P. S. S. R., Cunha, W. R, Araujo, O. P., Soares, M. G., Ferreira, M. S., Andrade, J. V., Miranda, G. S., Santos, M. F. C, Silva, M. L. A. e

“…Abstract Schistosomiasis, caused by Schistosoma mansoni Sambon, 1907, is a severe and widely distributed parasitic disease, affecting about 200 million people…”
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Metabolomics-based identification of apoptosis-inducing metabolites in recombinant fed-batch CHO culture media by Chong, William P.K., Yusufi, Faraaz N.K., Lee, Dong-Yup, Reddy, Satty G., Wong, Niki S.C., Heng, Chew Kiat, Yap, Miranda G.S., Ho, Ying Swan

“…A liquid chromatography–mass spectrometry (LC–MS) based metabolomics platform was previously established to identify and profile extracellular metabolites in…”
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Metabolomics profiling of extracellular metabolites in recombinant Chinese Hamster Ovary fed-batch culture by Chong, William P. K., Goh, Lin Tang, Reddy, Satty G., Yusufi, Faraaz N.K., Lee, Dong Yup, Wong, Niki S. C., Heng, Chew Kiat, Yap, Miranda G. S., Ho, Ying Swan

“…A metabolomics‐based approach was used to time profile extracellular metabolites in duplicate fed‐batch bioreactor cultures of recombinant Chinese Hamster…”
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Regulation of XBP-1 signaling during transient and stable recombinant protein production in CHO cells by Ku, Sebastian C. Y., Toh, Poh Choo, Lee, Yih Yean, Chusainow, Janet, Yap, Miranda G. S., Chao, Sheng-Hao

“…X‐box binding protein 1 (XBP‐1) is a key regulator of cellular unfolded protein response (UPR). The spliced isoform of XBP‐1, XBP‐1S, is a transcription…”
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Clinical Correlation of Transcription Factor SOX3 in Cancer: Unveiling Its Role in Tumorigenesis by Del Puerto, Helen Lima, Miranda, Ana Paula G S, Qutob, Dinah, Ferreira, Enio, Silva, Felipe H S, Lima, Bruna M, Carvalho, Barbara A, Roque-Souza, Bruna, Gutseit, Eduardo, Castro, Diego C, Pozzolini, Emanuele T, Duarte, Nayara O, Lopes, Thacyana B G, Taborda, Daiana Y O, Quirino, Stella M, Elgerbi, Ahmed, Choy, John S, Underwood, Adam

“…Members of the SOX (SRY-related HMG box) family of transcription factors are crucial for embryonic development and cell fate determination. This review…”
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Maximizing interferon-γ production by chinese hamster ovary cells through temperature shift optimization: Experimental and modeling by Fox, Stephen R., Patel, Upasana A., Yap, Miranda G. S., Wang, Daniel I. C.

“…The Chinese hamster ovary (CHO) cell line producing interferon‐γ (IFN‐γ) exhibits a 2‐fold increase in specific productivity when grown at 32°C compared to…”
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