Investigation of the rhythmic recruitment of tear neutrophils to the ocular surface and their phenotypes

Investigation of the rhythmic recruitment of tear neutrophils to the ocular surface and their phenotypes

Hundreds of thousands of polymorphonuclear neutrophils (PMNs) are collected from the ocular surface upon waking, while few are harvested during daytime. This study aimed to investigate potential factors contributing to the circadian infiltration of tear PMNs, including changes in IL-8 and C5a in tea...

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Bibliographic Details
Published in:Scientific reports Vol. 14; no. 1; p. 7061
Main Authors: Jin, Yutong, Minten, Ceili, Jenkins, Mara, Jones, Lyndon, Gorbet, Maud
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 25-03-2024
Nature Publishing Group
Nature Portfolio
Subjects:
631/250/2504
631/250/256
631/80/105
Circadian
Circadian rhythms
Closed-eye environment
Degranulation
Eye
Face
Humanities and Social Sciences
Interleukin 8
Leukocytes (neutrophilic)
Leukocytes (polymorphonuclear)
multidisciplinary
NETs
Neutrophils
Phenotype
Phenotypes
Science
Science (multidisciplinary)
Tears
Degranulation
NETs
Circadian
Closed-eye environment
Neutrophils
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Summary:Hundreds of thousands of polymorphonuclear neutrophils (PMNs) are collected from the ocular surface upon waking, while few are harvested during daytime. This study aimed to investigate potential factors contributing to the circadian infiltration of tear PMNs, including changes in IL-8 and C5a in tears, and their phenotypes across different time points in a 24-h cycle. Tear PMNs were collected using a gentle eyewash after 2-h and 7-h of sleep (eye closure, EC) at night, after 2-h EC during the day, and towards the end of the afternoon. Significantly fewer cells were collected after 2-h EC during the day compared to 2-h EC at night. A positive correlation between IL-8 and PMN numbers existed, but not with C5a. Tear PMNs collected after 2-h EC at night were less degranulated and possessed a larger activation potential compared to 7-h EC. Tear PMNs from 7-h EC at night exhibited hyper-segmented nuclei and more NETosis compared to 2 h EC night, indicating an aged and activated phenotype. The diurnal-nocturnal recruitment pattern of tear PMNs may be driven by increased IL-8 in nighttime tears. Higher degranulation and NETs point to the significant activation of tear PMNs on the ocular surface during prolonged eye closure at night.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-57311-8