Search Results - "Minegishi, Yasutaka"
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Rapid Screening Detection of Genetically Modified Papaya by Loop-Mediated Isothermal Amplification
Published in Biological & pharmaceutical bulletin (01-05-2023)“…A loop-mediated isothermal amplification (LAMP)-mediated screening detection method for genetically modified (GM) papaya was developed targeting the 35S…”
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Real-time PCR Detection Methods for Food Allergens (Wheat, Buckwheat, and Peanuts) Using Reference Plasmids
Published in Journal of agricultural and food chemistry (15-05-2019)“…Specific and sensitive real-time qualitative polymerase chain reaction (PCR) methods for the detection of food allergens including wheat, buckwheat, and…”
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Diverged and Active Partitiviruses in Lichen
Published in Frontiers in microbiology (21-10-2020)“…The lichen is a microbial consortium that mainly consists of fungi and either algae (Viridiplantae) or cyanobacteria. This structure also contains other…”
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Development of a Novel Detection Method Targeting an Ultrashort 25 bp Sequence Found in Agrobacterium-Mediated Transformed GM Plants
Published in Journal of agricultural and food chemistry (23-12-2020)“…Agrobacterium-mediated transformation is the most commonly used technique for plant genetic engineering. During the transformation, a T-DNA region, which is…”
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Development and evaluation of rapid screening detection methods for genetically modified crops using loop-mediated isothermal amplification
Published in Food chemistry (30-06-2018)“…•LAMP-mediated novel, comprehensive and rapid detection methods for GM maize and soybean events were developed.•The limit of detections for each target…”
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Qualitative real-time PCR identification of the khapra beetle, Trogoderma granarium (Coleoptera: Dermestidae)
Published in Applied entomology and zoology (15-02-2019)“…The identification of insect species is generally achieved by analyzing the specific morphological characteristics or, in more recent years, by polymerase…”
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Evaluation of a Species-specific, Stable, and Endogenous Sequence of Eggplant (Solanum melongena) using LAMP for the Detection of Genetically Modified Eggplants
Published in Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) (25-12-2021)“…Species-specific endogenous reference sequences are indispensable in the development of methods to detect genetically modified (GM) crops for food and feed. We…”
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Development and Interlaboratory Validation of Quantitative Polymerase Chain Reaction Method for Screening Analysis of Genetically Modified Soybeans
Published in Biological & pharmaceutical bulletin (01-01-2013)“…A novel real-time polymerase chain reaction (PCR)-based quantitative screening method was developed for three genetically modified soybeans: RRS, A2704-12, and…”
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Practicable Group Testing Method to Evaluate Weight/Weight GMO Content in Maize Grains
Published in Journal of agricultural and food chemistry (13-07-2011)“…Because of the increasing use of maize hybrids with genetically modified (GM) stacked events, the established and commonly used bulk sample methods for PCR…”
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Inter-Laboratory Study to Validate New Rapid Screening Methods for Kudoa septempunctata
Published in Biocontrol Science (2016)“…Kudoa septempunctata is the causative agent of a food-borne disease associated with the ingestion of raw olive flounder. As the current qRT-PCR method for its…”
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The Development of a Novel, Validated, Rapid and Simple Method for the Detection of Sarcocystis fayeri in Horse Meat in the Sanitary Control Setting
Published in Biocontrol Science (2016)“…Sarcocystis fayeri (S. fayeri) is a newly identified causative agent of foodborne disease that is associated with the consumption of raw horse meat. The…”
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Comprehensive GMO Detection Using Real-Time PCR Array: Single-Laboratory Validation
Published in Journal of AOAC International (01-03-2012)“…We have developed a real-time PCR array method to comprehensively detect genetically modified (GM) organisms. In the method, genomic DNA extracted from an…”
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Interlaboratory Study of Qualitative PCR Methods for Genetically Modified Maize Events MON810, Bt11, GA21, and CaMV P35S
Published in Journal of AOAC International (01-03-2013)“…Qualitative PCR methods for the genetically modified (GM) maize events MON810, Bt11, and GA21, and the 35S promoter (P35S) region of the cauliflower mosaic…”
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LAMP法による遺伝子組換えナス検出のための,種特異的かつ安定なナス内在性配列の開発
Published in Shokuhin eiseigaku zasshi (25-12-2021)“…食品あるいは飼料において,遺伝子組換え(GM)作物の検出法を開発するためには,種特異的な内在性配列が不可欠である.本研究では,ナス科作物のβ-fructosidase遺伝子の部分配列を用いて,loop-mediated isothermal amplification…”
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Selection of Suitable DNA Extraction Methods for Genetically Modified Maize 3272, and Development and Evaluation of an Event-Specific Quantitative PCR Method for 3272
Published in Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) (25-02-2016)“…A novel real-time PCR-based analytical method was developed for the event-specific quantification of a genetically modified (GM) maize, 3272. We first…”
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Development and Evaluation of Event-Specific Quantitative PCR Method for Genetically Modified Soybean MON87701
Published in Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) (25-12-2016)“…A real-time PCR-based analytical method was developed for the event-specific quantification of a genetically modified (GM) soybean event, MON87701. First, a…”
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Evaluation of Modified PCR Quantitation of Genetically Modified Maize and Soybean Using Reference Molecules: Interlaboratory Study
Published in Journal of AOAC International (2009)“…Real-time polymerase chain reaction (PCR)-based quantitative methods were previously developed and validated for genetically modified (GM) maize or soy. In…”
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Development and Validation of an Event-Specific Quantitative PCR Method for Genetically Modified Maize MIR162
Published in Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) (25-10-2014)“…A novel real-time PCR-based analytical method was developed for the event-specific quantification of a genetically modified (GM) maize event, MIR162. We first…”
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Development of a Screening Method for Genetically Modified Soybean by Plasmid-Based Quantitative Competitive Polymerase Chain Reaction
Published in Journal of agricultural and food chemistry (23-07-2008)“…A novel type of quantitative competitive polymerase chain reaction (QC-PCR) system for the detection and quantification of the Roundup Ready soybean (RRS) was…”
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