Comparison of the removal of 2,4-dichlorophenol and phenol from polluted water, by peroxidases from tomato hairy roots, and protective effect of polyethylene glycol

Multiple efforts have been directed towards optimized processes in which enzymes, like peroxidases, are used to remove phenolic compounds from polluted wastewater. Here we describe the use of peroxidase isoenzymes from tomato hairy roots, which were able to oxidise 2,4-dichlorophenol (2,4-DCP) and p...

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Bibliographic Details
Published in:	Chemosphere (Oxford) Vol. 70; no. 6; pp. 982 - 989
Main Authors:	González, Paola Solange, Agostini, Elizabeth, Milrad, Silvia Raquel
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 2008 Elsevier
Subjects:	2,4-D acidic peroxidase Additives Applied sciences basic peroxidase biodegradation Biological and medical sciences Biological and physicochemical phenomena Biological treatment of waters Bioremediation Biotechnology Chlorophenols - isolation & purification Chlorophenols - metabolism Environment and pollution Enzyme Activation - drug effects enzyme activity Exact sciences and technology Fundamental and applied biological sciences. Psychology General purification processes genetic transformation Hairy roots hydrogen peroxide Industrial applications and implications. Economical aspects ionically bound to cell wall peroxidase Isoenzymes - metabolism Lycopersicon esculentum Natural water pollution PEG peroxidase Peroxidase - metabolism Peroxidase isoenzymes phenol Phenol - isolation & purification Phenol - metabolism Phenolic compounds phytoremediation Plant Proteins - metabolism Plant Roots - enzymology Pollution polyethylene glycol Polyethylene Glycols - pharmacology Rhizobium rhizogenes roots Solanum lycopersicum var. lycopersicum tomatoes transgenic plants wastewater treatment Wastewaters Water Pollutants, Chemical - isolation & purification Water Pollution, Chemical - prevention & control Water treatment and pollution Peroxidase isoenzymes Additives PEG Bioremediation Phenolic compounds Hairy roots Hairy root Isozyme Enzymatic catalysis Biological purification Ethylene oxide polymer Additive Decontamination Dicotyledones Angiospermae Lycopersicon esculentum Peroxidase Solanaceae Organic compounds Enzyme Polymerization Organic chlorine compounds Operating conditions Phytoremediation Peroxidases Plant origin Phenols Spermatophyta Water pollution Oxidoreductases Waste water purification
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Summary:	Multiple efforts have been directed towards optimized processes in which enzymes, like peroxidases, are used to remove phenolic compounds from polluted wastewater. Here we describe the use of peroxidase isoenzymes from tomato hairy roots, which were able to oxidise 2,4-dichlorophenol (2,4-DCP) and phenol from aqueous solutions. This could be an interesting alternative for the removal of these compounds from contaminated sites. We used different enzyme fractions: total peroxidases (TP), ionically bound to cell wall peroxidases (IBP), basic (BP) and acidic peroxidases (AP). We analyzed the optimum conditions of removal, the effect of Polyethyleneglycol (PEG-3350) on the process and on the enzyme activities, to obtain the maximum efficiency. The optimal H 2O 2 concentrations for 2,4-DCP and phenol removal were 1 and 0.1 mM, respectively. TP, IBP and BP showed better removal efficiencies than AP, for both contaminants. The addition of different concentrations (10–100 mg l −1) of PEG-3350 to solutions containing 2,4-DCP showed no effect on the removal efficiencies of the isoenzymes. However, PEG (100 mg l −1) increased the removal efficiency of phenol by BP and IBP fractions. On the other hand, peroxidase activities from BP and IBP fractions were 3 and 13 times higher, respectively, than those detected for the same fractions in phenol treated solutions without PEG. The protective effect of PEG, which depends on the contaminant as well as of the enzyme fraction used, would be important to improve the removal efficiency of phenol by some peroxidase isoenzymes.
Bibliography:	http://dx.doi.org/10.1016/j.chemosphere.2007.08.025 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0045-6535 1879-1298
DOI:	10.1016/j.chemosphere.2007.08.025