Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides

Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides

Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola popu...

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Bibliographic Details
Published in:European journal of plant pathology Vol. 149; no. 4; pp. 895 - 910
Main Authors: Trkulja, Nenad R., Milosavljević, Anja G., Mitrović, Milana S., Jović, Jelena B., Toševski, Ivo T., Khan, Mohamed F. R., Secor, Gary A.
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-12-2017
Springer Nature B.V
Subjects:
Agriculture
Benzimidazoles
Beta vulgaris
Biomedical and Life Sciences
COB gene
CYP51 gene
Demethylation
Ecology
Fungicides
Haplotypes
Leafspot
Life Sciences
Mutation
Pesticides
Plant Pathology
Plant Sciences
Populations
Quinones
Sugar
Tubulin
ß-tubulin
Multi-resistance
Molecular characterization
CYP51
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Summary:Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob , CYP51 and ß-tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the ß-tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 ( N  = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 ( N  = 11, QoI-S, DMI-R and MBC-R); and MR3 ( N  = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-017-1239-0