Search Results - "Miller, Shondra"

CRIS.py: A Versatile and High-throughput Analysis Program for CRISPR-based Genome Editing by Connelly, Jon P., Pruett-Miller, Shondra M.

“…CRISPR-Cas9 technology allows the creation of user-defined genomic modifications in cells and whole organisms. However, quantifying editing rates in pools of…”
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A Survey of Validation Strategies for CRISPR-Cas9 Editing by Sentmanat, Monica F., Peters, Samuel T., Florian, Colin P., Connelly, Jon P., Pruett-Miller, Shondra M.

“…The T7 endonuclease 1 (T7E1) mismatch detection assay is a widely used method for evaluating the activity of site-specific nucleases, such as the clustered…”
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Identification of Potent, Selective, and Orally Bioavailable Small-Molecule GSPT1/2 Degraders from a Focused Library of Cereblon Modulators by Nishiguchi, Gisele, Keramatnia, Fatemeh, Min, Jaeki, Chang, Yunchao, Jonchere, Barbara, Das, Sourav, Actis, Marisa, Price, Jeanine, Chepyala, Divyabharathi, Young, Brandon, McGowan, Kevin, Slavish, P. Jake, Mayasundari, Anand, Jarusiewicz, Jamie A, Yang, Lei, Li, Yong, Fu, Xiang, Garrett, Shalandus H, Papizan, James B, Kodali, Kiran, Peng, Junmin, Pruett Miller, Shondra M, Roussel, Martine F, Mullighan, Charles, Fischer, Marcus, Rankovic, Zoran

“…Whereas the PROTAC approach to target protein degradation greatly benefits from rational design, the discovery of small-molecule degraders relies mostly on…”
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The Zα2 domain of ZBP1 is a molecular switch regulating influenza-induced PANoptosis and perinatal lethality during development by Kesavardhana, Sannula, Malireddi, R. K. Subbarao, Burton, Amanda R., Porter, Shaina N., Vogel, Peter, Pruett-Miller, Shondra M., Kanneganti, Thirumala-Devi

“…Z-DNA-binding protein 1 (ZBP1) is an innate immune sensor of nucleic acids that regulates host defense responses and development. ZBP1 activation triggers…”
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Discovery of a proteinaceous cellular receptor for a norovirus by Orchard, Robert C., Wilen, Craig B., Doench, John G., Baldridge, Megan T., McCune, Broc T., Lee, Ying-Chiang J., Lee, Sanghyun, Pruett-Miller, Shondra M., Nelson, Christopher A., Fremont, Daved H., Virgin, Herbert W.

“…Noroviruses (NoVs) are a leading cause of gastroenteritis globally, yet the host factors required for NoV infection are poorly understood. We identified host…”
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cBAF complex components and MYC cooperate early in CD8+ T cell fate by Guo, Ao, Huang, Hongling, Zhu, Zhexin, Chen, Mark J., Shi, Hao, Yuan, Sujing, Sharma, Piyush, Connelly, Jon P., Liedmann, Swantje, Dhungana, Yogesh, Li, Zhenrui, Haydar, Dalia, Yang, Mao, Beere, Helen, Yustein, Jason T., DeRenzo, Christopher, Pruett-Miller, Shondra M., Crawford, Jeremy Chase, Krenciute, Giedre, Roberts, Charles W. M., Chi, Hongbo, Green, Douglas R.

“…The identification of mechanisms to promote memory T (T mem ) cells has important implications for vaccination and anti-cancer immunotherapy 1 – 4 . Using a…”
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Deleting DNMT3A in CAR T cells prevents exhaustion and enhances antitumor activity by Prinzing, Brooke, Zebley, Caitlin C, Petersen, Christopher T, Fan, Yiping, Anido, Alejandro Allo, Yi, Zhongzhen, Nguyen, Phuong, Houke, Haley, Bell, Matthew, Haydar, Dalia, Brown, Charmaine, Boi, Shannon K, Alli, Shanta, Crawford, Jeremy Chase, Riberdy, Janice M, Park, Jeoungeun J, Zhou, Sheng, Velasquez, Mireya Paulina, DeRenzo, Chris, Lazzarotto, Cicera R, Tsai, Shengdar Q, Vogel, Peter, Pruett-Miller, Shondra M, Langfitt, Deanna M, Gottschalk, Stephen, Youngblood, Ben, Krenciute, Giedre

“…Chimeric antigen receptor (CAR) T cell therapy is revolutionizing cancer immunotherapy for patients with B cell malignancies and is now being developed for…”
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ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97 by Wang, Bo, Maxwell, Brian A., Joo, Joung Hyuck, Gwon, Youngdae, Messing, James, Mishra, Ashutosh, Shaw, Timothy I., Ward, Amber L., Quan, Honghu, Sakurada, Sadie Miki, Pruett-Miller, Shondra M., Bertorini, Tulio, Vogel, Peter, Kim, Hong Joo, Peng, Junmin, Taylor, J. Paul, Kundu, Mondira

“…Disturbances in autophagy and stress granule dynamics have been implicated as potential mechanisms underlying inclusion body myopathy (IBM) and related…”
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High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases by Chen, Fuqiang, Pruett-Miller, Shondra M, Huang, Yuping, Gjoka, Monika, Duda, Katarzyna, Taunton, Jack, Collingwood, Trevor N, Frodin, Morten, Davis, Gregory D

“…Single-stranded oligonucleotides are used as donor templates for zinc-finger nucleases to create targeted genomic deletions and mutations in mammalian cell…”
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Positional cloning of quantitative trait nucleotides for blood pressure and cardiac QT-interval by targeted CRISPR/Cas9 editing of a novel long non-coding RNA by Cheng, Xi, Waghulde, Harshal, Mell, Blair, Morgan, Eric E, Pruett-Miller, Shondra M, Joe, Bina

“…Multiple GWAS studies have reported strong association of cardiac QT-interval to a region on HSA17. Interestingly, a rat locus homologous to this region is…”
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High-Throughput Analysis of CRISPR-Cas9 Editing Outcomes in Cell and Animal Models Using CRIS.py by Narina, Shilpa, Connelly, Jon P, Pruett-Miller, Shondra M

“…Genome editing using the CRISPR-Cas9 platform creates precise modifications in cells and whole organisms. Although knockout (KO) mutations can occur at high…”
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Ca2+-mediated mitochondrial inner membrane permeabilization induces cell death independently of Bax and Bak by Quarato, Giovanni, Llambi, Fabien, Guy, Cliff S., Min, Jaeki, Actis, Marisa, Sun, Huan, Narina, Shilpa, Pruett-Miller, Shondra M., Peng, Junmin, Rankovic, Zoran, Green, Douglas R.

“…The ability of mitochondria to buffer a rapid rise in cytosolic Ca 2+ is a hallmark of proper cell homeostasis. Here, we employed m-3M3FBS, a putative…”
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Gain-of-function mutations in RPA1 cause a syndrome with short telomeres and somatic genetic rescue by Sharma, Richa, Sahoo, Sushree S., Honda, Masayoshi, Granger, Sophie L., Goodings, Charnise, Sanchez, Louis, Künstner, Axel, Busch, Hauke, Beier, Fabian, Pruett-Miller, Shondra M., Valentine, Marcus B., Fernandez, Alfonso G., Chang, Ti-Cheng, Géli, Vincent, Churikov, Dmitri, Hirschi, Sandrine, Pastor, Victor B., Boerries, Melanie, Lauten, Melchior, Kelaidi, Charikleia, Cooper, Megan A., Nicholas, Sarah, Rosenfeld, Jill A., Polychronopoulou, Sophia, Kannengiesser, Caroline, Saintomé, Carole, Niemeyer, Charlotte M., Revy, Patrick, Wold, Marc S., Spies, Maria, Erlacher, Miriam, Coulon, Stéphane, Wlodarski, Marcin W.

“…Human telomere biology disorders (TBD)/short telomere syndromes (STS) are heterogeneous disorders caused by inherited loss-of-function mutations in…”
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Pediatric MDS and bone marrow failure-associated germline mutations in SAMD9 and SAMD9L impair multiple pathways in primary hematopoietic cells by Thomas, Melvin E., Abdelhamed, Sherif, Hiltenbrand, Ryan, Schwartz, Jason R., Sakurada, Sadie Miki, Walsh, Michael, Song, Guangchun, Ma, Jing, Pruett-Miller, Shondra M., Klco, Jeffery M.

“…Pediatric myelodysplastic syndromes (MDS) are a heterogeneous disease group associated with impaired hematopoiesis, bone marrow hypocellularity, and frequently…”
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Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer by Zhou, Peipei, Shi, Hao, Huang, Hongling, Sun, Xiang, Yuan, Sujing, Chapman, Nicole M., Connelly, Jon P., Lim, Seon Ah, Saravia, Jordy, KC, Anil, Pruett-Miller, Shondra M., Chi, Hongbo

“…CD8 + cytotoxic T cells (CTLs) orchestrate antitumour immunity and exhibit inherent heterogeneity 1 , 2 , with precursor exhausted T (T pex ) cells but not…”
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14-3-3ζ–TRAF5 axis governs interleukin-17A signaling by McGowan, Jenna, Peter, Cara, Kim, Joshua, Popli, Sonam, Veerman, Brent, Saul-McBeth, Jessica, Conti, Heather, Pruett-Miller, Shondra M., Chattopadhyay, Saurabh, Chakravarti, Ritu

“…IL-17A is a therapeutic target in many autoimmune diseases. Most nonhematopoietic cells express IL-17A receptors and respond to extracellular IL-17A by…”
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Caspase-6 promotes activation of the caspase-11-NLRP3 inflammasome during gram-negative bacterial infections by Zheng, Min, Karki, Rajendra, Kancharana, Balabhaskararao, Berns, Hartmut, Pruett-Miller, Shondra M., Kanneganti, Thirumala-Devi

“…The innate immune system acts as the first line of defense against infection. One key component of the innate immune response to gram-negative bacterial…”
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A Cancer-Specific Ubiquitin Ligase Drives mRNA Alternative Polyadenylation by Ubiquitinating the mRNA 3′ End Processing Complex by Yang, Seung Wook, Li, Lei, Connelly, Jon P., Porter, Shaina N., Kodali, Kiran, Gan, Haiyun, Park, Jung Mi, Tacer, Klementina Fon, Tillman, Heather, Peng, Junmin, Pruett-Miller, Shondra M., Li, Wei, Potts, Patrick Ryan

“…Alternative polyadenylation (APA) contributes to transcriptome complexity by generating mRNA isoforms with varying 3′ UTR lengths. APA leading to 3′ UTR…”
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Attenuation of Microbiotal Dysbiosis and Hypertension in a CRISPR/Cas9 Gene Ablation Rat Model of GPER1 by Waghulde, Harshal, Cheng, Xi, Galla, Sarah, Mell, Blair, Cai, Jingwei, Pruett-Miller, Shondra M, Vazquez, Guillermo, Patterson, Andrew, Vijay Kumar, Matam, Joe, Bina

“…G-protein–coupled estrogen receptor, Gper1, has been implicated in cardiovascular disease, but its mechanistic role in blood pressure control is poorly…”
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Fragmid: A toolkit for rapid assembly and assessment of CRISPR technologies by Pruett-Miller, Shondra M.

“…The CRISPR toolbox continues to expand at a rapid pace, leaving researchers scrambling to assess the latest tools in their systems of interest. McGee et al.1…”
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