ALGORITHM FOR LABORATORY DIAGNOSTICS OF CLOSTRIDIUM DIFFICILE ASSOCIATED DIARRHEA

Aim. To develop an optimal algorithm for laboratory diagnosis of C. difficile associated diarrhea in context of obtaining the most reliable analysis results. Materials and methods. 211 patients with clinically significant C. difficile associated diarrhea participated in the study. Luminal faeces wer...

Full description

Saved in:

Bibliographic Details
Published in:	Žurnal mikrobiologii, ėpidemiologii i immunobiologii Vol. 95; no. 2; pp. 45 - 53
Main Authors:	Sukhina, M. A., Obraztsov, I. V., Mikhalevskaya, V. I., Achkasov, S. I., Safin, A. L., Shelygin, Yu. A.
Format:	Journal Article
Language:	English
Published:	Central Research Institute for Epidemiology 28-04-2018
Subjects:	antibiotic-associated diarrhea binary toxin c. difficile associated infection c. difficile-ассоциированная инфекция glutamatedehydrogenase sensitivity specificity toxin a toxin b
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Aim. To develop an optimal algorithm for laboratory diagnosis of C. difficile associated diarrhea in context of obtaining the most reliable analysis results. Materials and methods. 211 patients with clinically significant C. difficile associated diarrhea participated in the study. Luminal faeces were analysed by immunological (immune chromatographic assay, ICA; ELISA) bacteriological and molecular (GENEexpert rtPCR system, Cepheid, USA) methods. Results. We isolated 126 C. difficile strains from 211 samples of luminal faeces. We identified glutamate dehydrogenase (GDH) in 54% of cases (n=68) by means of immune ICA, and in 11.1% of cases (n=14) by means of ELISA. The increase of bacterial concentration is associated with the growth of sensitivity of GDH detection by immunological tests (p
ISSN:	0372-9311 2686-7613
DOI:	10.36233/0372-9311-2018-2-45-53