Search Results - "Mierendorf, Robert C."
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A novel transcription property of SP6 and T7 RNA polymerases: dependence on template structure
Published in Nucleic acids research (11-09-1985)“…The in vitro synthesis of extraneous RNA sequences by SP6 and T7 RNA polymerases from specific DNA templates is described. Transcription of templates prepared…”
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2
Phage lambda cDNA cloning vectors for subtractive hybridization, fusion-protein synthesis and Cre-loxP automatic plasmid subcloning
Published in Gene (30-03-1990)“…We describe the construction and use of two classes of cDNA cloning vectors. The first class comprises the lambda EXLX(+) and lambda EXLX(-) vectors that can…”
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3
Functional heterogeneity of monoclonal antibodies obtained using different screening assays
Published in Analytical biochemistry (01-11-1983)“…Two alternate screening methods have enabled the detection of monoclonal antibodies with different specificities toward the lysosomal enzyme alpha-mannosidase…”
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4
Direct sequencing of denatured plasmid DNA
Published in Methods in enzymology (1987)Get more information
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5
Gene isolation by screening lambda gt11 libraries with antibodies
Published in Methods in enzymology (1987)Get more information
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6
Sequencing of RNA transcripts synthesized in vitro from plasmids containing bacteriophage promoters
Published in Methods in enzymology (1987)Get more information
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7
Pathways Involved in Targeting and Secretion of a Lysosomal Enzyme in Dictyostelium discoideum
Published in The Journal of cell biology (01-05-1985)“…In Dictyostelium discoideum, the lysosomal enzyme α-mannosidase is first synthesized as an N-glycosylated precursor of Mr 140,000. After a 20-30-min lag…”
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Defining the intracellular localization pathways followed by lysosomal enzymes in Dictyostelium discoideum
Published in Methods in cell biology (1987)Get more information
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9
Initial events involved in the synthesis of the lysosomal enzyme alpha-mannosidase in Dictyostelium discoideum
Published in Archives of biochemistry and biophysics (01-01-1986)“…In Dictyostelium discoideum the lysosomal enzyme alpha-mannosidase is initially synthesized in vivo as a 140,000 Mr protein which is subsequently processed…”
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10
Regulation of lysosomal α-mannosidase-1 synthesis during development in Dictyostelium discoideum
Published in Developmental biology (01-01-1985)“…The cellular specific activity of lysosomal α-mannosidase-1 increases dramatically during development in Dictyostelium discoideum. α-Mannosidase-1 is composed…”
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11
Possible role of glucocorticoid receptors in globin gene expression in differentiating Friend cells
Published in Molecular and cellular endocrinology (01-03-1979)“…Previous studies from this laboratory have shown that the expression of globin genes during the dimethyl sulfoxide-induced differentiation of T3C12 Friend…”
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12
Immunological recognition of modifications on functionally related proteins
Published in Methods in enzymology (1983)Get more information
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