Search Results - "Mie, Masayasu"

Development of an enhanced immunoassay based on protein nanoparticles displaying an IgG-binding domain and luciferase by Wang, Gaoyang, Mashimo, Yasumasa, Kobatake, Eiry, Mie, Masayasu

“…Detection of small amounts of target molecules with high sensitivity is important for the diagnosis of many diseases, including cancers, and is particularly…”
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Construction of DNA-NanoLuc luciferase conjugates for DNA aptamer-based sandwich assay using Rep protein by Mie, Masayasu, Niimi, Takahiro, Mashimo, Yasumasa, Kobatake, Eiry

“…Objective We developed a DNA-NanoLuc luciferase (NnaoLuc) conjugates for DNA aptamer-based sandwich assay using the catalytic domain of the replication…”
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Development and performance evaluation of a rapid in-house ELISA for retrospective serosurveillance of SARS-CoV-2 by Sil, Bijon Kumar, Jahan, Nowshin, Haq, Md Ahsanul, Oishee, Mumtarin Jannat, Ali, Tamanna, Khandker, Shahad Saif, Kobatake, Eiry, Mie, Masayasu, Khondoker, Mohib Ullah, Jamiruddin, Mohd Raeed, Adnan, Nihad

“…In the ongoing pandemic situation of COVID-19, serological tests can complement the molecular diagnostic methods, and can be one of the important tools of…”
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Growth Factor Tethering to Protein Nanoparticles via Coiled-Coil Formation for Targeted Drug Delivery by Assal, Yasmine, Mizuguchi, Yoshinori, Mie, Masayasu, Kobatake, Eiry

“…Protein-based nanoparticles are attractive carriers for drug delivery because they are biodegradable and can be genetically designed. Moreover, modification of…”
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Direct Labeling of Protein Nanoparticles with Fluorescent Compounds for Immunoassay Applications by SUGIHARA, Tsutomu, MIE, Masayasu, KOBATAKE, Eiry

“…A fusion protein, designated ELP-D-C, comprised of a hydrophobic elastin-like polypeptide unit, a hydrophilic aspartic acid-rich peptide unit, and an…”
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Application of elastin-based nanoparticles displaying antibody binding domains for a homogeneous immunoassay by Sugihara, Tsutomu, Mie, Masayasu, Kobatake, Eiry

“…Nanoparticles are small size-controlled particles from 1 to 100 nm diameters and characterized by their structure, base material and functional units displayed…”
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Construction of nanoscale protein particle using temperature-sensitive elastin-like peptide and polyaspartic acid chain by Fujita, Yoshihiko, Mie, Masayasu, Kobatake, Eiry

“…Abstract Temperature-responsive monodisperse spheres are useful for various in vivo and in vitro applications. Size, response temperature and biocompatibility…”
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Selection of DNA aptamers recognizing small cell lung cancer using living cell-SELEX by Kunii, Takao, Ogura, Shun-ichiro, Mie, Masayasu, Kobatake, Eiry

“…We applied Systematic Evolution of Ligands by EXponential enrichment using Small Cell Lung Cancer (SCLC) cells. A DNA aptamer was identified and evaluated by…”
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Design of luciferase-displaying protein nanoparticles for use as highly sensitive immunoassay detection probes by Ikeda, Yusuke, Mashimo, Yasumasa, Mie, Masayasu, Kobatake, Eiry

“…In this study, we developed a protein nanoparticle-based immunoassay to detect cancer biomarkers using a bioluminescent fusion protein. This method relies on…”
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Development of drug-loaded protein nanoparticles displaying enzymatically-conjugated DNA aptamers for cancer cell targeting by Mie, Masayasu, Matsumoto, Rie, Mashimo, Yasumasa, Cass, Anthony E. G., Kobatake, Eiry

“…Modification of protein-based drug carriers with tumor-targeting properties is an important area of research in the field of anticancer drug delivery. To this…”
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Tracking a protein following dissociation from a protein–protein complex using a split SNAP-tag system by Mie, Masayasu, Naoki, Tatsuhiko, Kobatake, Eiry

“…Protein–protein interactions (PPIs) are important for various biological processes in living cells. Several methods have been developed for the visualization…”
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Construction of an Enzymatically-Conjugated DNA Aptamer–Protein Hybrid Molecule for Use as a BRET-Based Biosensor by Mie, Masayasu, Hirashima, Rena, Mashimo, Yasumasa, Kobatake, Eiry

“…DNA-protein conjugates are useful molecules for construction of biosensors. Herein, we report the development of an enzymatically-conjugated DNA…”
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Construction of a Defined Biomimetic Matrix for Long-Term Maintenance of Mouse Induced Pluripotent Stem Cells by Adnan, Nihad, Mie, Masayasu, Haque, Amranul, Hossain, Sharif, Mashimo, Yasumasa, Akaike, Toshihiro, Kobatake, Eiry

“…The existing in vitro culture systems often use undefined and animal-derived components for the culture of pluripotent stem cells. Artificial bioengineered…”
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Systematic screening of the cellular uptake of designed alpha-helix peptides by Usui, Kenji, Kikuchi, Takuya, Mie, Masayasu, Kobatake, Eiry, Mihara, Hisakazu

“…The cellular penetration (CP) activity of functional molecules has attracted significant attention as one of the most promising new approaches for drug…”
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Induction of motor neuron differentiation by transduction of Olig2 protein by Mie, Masayasu, Kaneko, Mami, Henmi, Fumiaki, Kobatake, Eiry

“…► Olig2 protein has the ability to permeate the cell membrane. ► By addition of Olig2 protein, P19 cells showed elongation of neurites. ► Olig2 protein induced…”
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Delivery of bFGF for Tissue Engineering by Tethering to the ECM by Kobatake, Eiry, Mie, Masayasu, Mashimo, Yasumasa, Suttinont, Chawapun

“…Delivery of growth factors to target cells is an important subject in tissue engineering. Towards that end, we have developed a growth factor-tethered…”
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Aptamer-based protein detection using a bioluminescent fusion protein by Akter, Farhima, Mie, Masayasu, Kobatake, Eiry

“…An aptamer-based sandwich-type immunoassay is presented to detect human thrombin using a bioluminescent fusion protein, SSB-fLuc. Escherichia coli…”
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Detection of small RNA molecules by a combination of branched rolling circle amplification and bioluminescent pyrophosphate assay by Mashimo, Yasumasa, Mie, Masayasu, Suzuki, Shigeya, Kobatake, Eiry

“…Aberrant expression of miRNAs often correlates with various human diseases. Therefore, miRNAs have been focused as disease biomarkers. Here, a novel…”
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Construction of a bFGF-tethered multi-functional extracellular matrix protein through coiled-coil structures for neurite outgrowth induction by Mie, Masayasu, Sasaki, Shoichi, Kobatake, Eiry

“…In this study, an artificial multi-functional extracellular matrix (ECM) protein, tethered with a growth factor, was developed for neurite outgrowth induction…”
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Construction of a bFGF-Tethered Extracellular Matrix Using a Coiled-Coil Helical Interaction by Kobatake, Eiry, Takahashi, Ryota, Mie, Masayasu

“…A novel method for construction of biomaterials for tissue engineering was developed. Noncovalent associations between extracellular matrix (ECM) and growth…”
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