Control of phenylalanine ammonia-lyase gene promoters from pea by UV radiation

Control of phenylalanine ammonia-lyase gene promoters from pea by UV radiation

The gene fusion system was used to study UV light-control of PS PAL1 and PS PAL2 genes encoding phenylalanine ammonia-lyase of pea. The induction of pea PAL promoters was analysed in transgenic tobacco plants. Binary plasmids (derivatives of pBI101.2 vector) containing 5' regulatory fragments o...

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Bibliographic Details
Published in:Acta physiologiae plantarum Vol. 27; no. 2; pp. 229 - 236
Main Authors: Pluskota, W.E, Michalczyk, D.J, Gorecki, R.J.,University of Warmia and Mazury, Olsztyn (Poland). Dept. of Plant Physiology and Biotechnology
Format: Journal Article
Language:English
Published: 01-01-2005
Subjects:
AMONIO FENILALANINA LIASA
GENE
GENES
GUISANTE
http://www.fao.org/aos/agrovoc#c_13177
http://www.fao.org/aos/agrovoc#c_29522
http://www.fao.org/aos/agrovoc#c_3214
http://www.fao.org/aos/agrovoc#c_4788
http://www.fao.org/aos/agrovoc#c_5933
http://www.fao.org/aos/agrovoc#c_6422
http://www.fao.org/aos/agrovoc#c_8046
METHODE
METHODS
METODOS
PEAS
PHENYLALANINE AMMONIA LYASE
PISUM SATIVUM
POIS
RADIACION
RADIACION ULTRAVIOLETA
RADIATION
RAYONNEMENT ULTRAVIOLET
ULTRAVIOLET RADIATION
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Summary:The gene fusion system was used to study UV light-control of PS PAL1 and PS PAL2 genes encoding phenylalanine ammonia-lyase of pea. The induction of pea PAL promoters was analysed in transgenic tobacco plants. Binary plasmids (derivatives of pBI101.2 vector) containing 5' regulatory fragments of PS PAL1 and PS PAL2 linked to reporter genes (GUS, LUC) were constructed. The analyses were performed with the use of single constructs (containing one variant of PS PAL promoter and one reporter gene) and dual constructs (containing both PS PAL1 and PS PAL2 promoters connected with different reporter genes). The use of dual constructs enabled the evaluation of both PS PAL promoters activity in the same plant. The analyses of in vitro grown plants have shown that both PAL promoters are strongly induced in leaves subjected to UV radiation. In some cases, the UV-stimulated expression exceeded the exposed areas. This phenomenon was observed more often in the leaves of plants containing the PS PAL1::GUS than PS PAL2::GUS construct. Removal of boxes 2, 4, 5 from PS PAL1 promoter and deletion of its 5' end region (-339 to -1394) decreases the level of gene expression but does not eliminate its responsiveness to UV
Bibliography:F60
2009000799
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-005-0027-1