Search Results - "Michael R. Kuehn"

Nodal signaling recruits the histone demethylase Jmjd3 to counteract polycomb-mediated repression at target genes by Dahle, Øyvind, Kumar, Amit, Kuehn, Michael R

“…Both intercellular signaling and epigenetic mechanisms regulate embryonic development, but it is unclear how they are integrated to establish and maintain…”
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Essential role of chromatin remodeling protein Bptf in early mouse embryos and embryonic stem cells by Landry, Joseph, Sharov, Alexei A, Piao, Yulan, Sharova, Lioudmila V, Xiao, Hua, Southon, Eileen, Matta, Jennifer, Tessarollo, Lino, Zhang, Ying E, Ko, Minoru S H, Kuehn, Michael R, Yamaguchi, Terry P, Wu, Carl

“…We have characterized the biological functions of the chromatin remodeling protein Bptf (Bromodomain PHD-finger Transcription Factor), the largest subunit of…”
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Nodal signaling from the visceral endoderm is required to maintain Nodal gene expression in the epiblast and drive DVE/AVE migration by Kumar, Amit, Lualdi, Margaret, Lyozin, George T., Sharma, Prashant, Loncarek, Jadranka, Fu, Xin-Yuan, Kuehn, Michael R.

“…In the early mouse embryo, a specialized population of extraembryonic visceral endoderm (VE) cells called the distal VE (DVE) arises at the tip of the egg…”
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Nedd4-binding partner 1 (N4BP1) protein is an inhibitor of the E3 ligase Itch by Oberst, Andrew, Malatesta, Martina, Aqeilan, Rami I, Rossi, Mario, Salomoni, Paolo, Murillas, Rodolfo, Sharma, Prashant, Kuehn, Michael R, Oren, Moshe, Croce, Carlo M, Bernassola, Francesca, Melino, Gerry

“…Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3…”
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Senp1 Is Essential for Desumoylating Sumo1-Modified Proteins but Dispensable for Sumo2 and Sumo3 Deconjugation in the Mouse Embryo by Sharma, Prashant, Yamada, Satoru, Lualdi, Margaret, Dasso, Mary, Kuehn, Michael R.

“…Posttranslational modification with small ubiquitin-like modifier (Sumo) regulates numerous cellular and developmental processes. Sumoylation is dynamic with…”
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Hofmeister series salts enhance purification of plasmid DNA by non-ionic detergents by Lezin, George, Kuehn, Michael R., Brunelli, Luca

“…Ion‐exchange chromatography is the standard technique used for plasmid DNA purification, an essential molecular biology procedure. Non‐ionic detergents (NIDs)…”
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A one-step miniprep for the isolation of plasmid DNA and lambda phage particles by Lezin, George, Kosaka, Yasuhiro, Yost, H Joseph, Kuehn, Michael R, Brunelli, Luca

“…Plasmid DNA minipreps are fundamental techniques in molecular biology. Current plasmid DNA minipreps use alkali and the anionic detergent SDS in a…”
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Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis by Lyozin, George T, Bressloff, Paul C, Kumar, Amit, Kosaka, Yasuhiro, Demarest, Bradley L, Yost, H Joseph, Kuehn, Michael R, Brunelli, Luca

“…Application of the founder principle from population genetics to variant selection after recombineering allows the isolation of rare unselected recombinants…”
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Mutation of SENP1/SuPr-2 Reveals an Essential Role for Desumoylation in Mouse Development by Yamaguchi, Taihei, Sharma, Prashant, Athanasiou, Meropi, Kumar, Amit, Yamada, Satoru, Kuehn, Michael R.

“…Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Broad mesodermal and endodermal deletion of Nodal at postgastrulation stages results solely in left/right axial defects by Kumar, Amit, Lualdi, Margaret, Lewandoski, Mark, Kuehn, Michael R.

“…Nodal signaling is a critical regulator of multiple aspects of early vertebrate development including asymmetry along the left/right (LR) axis. To study Nodal…”
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FGFR1 function at the earliest stages of mouse limb development plays an indispensable role in subsequent autopod morphogenesis by Li, Cuiling, Xu, Xiaoling, Nelson, Danielle K, Williams, Trevor, Kuehn, Michael R, Deng, Chu-Xia

“…Fibroblast growth factors (FGFs) and their receptors have been implicated in limb development. However, because of early post-implantation lethality associated…”
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Transgenic mouse lines expressing Cre recombinase specifically in posterior notochord and notochord by Kumar, Amit, Yamaguchi, Taihei, Sharma, Prashant, Kuehn, Michael R.

“…During development, the organizer provides instructive signals to surrounding cells as well as contributing cells to axial structures. To dissect organizer…”
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Loss of SUMO1 in mice affects RanGAP1 localization and formation of PML nuclear bodies, but is not lethal as it can be compensated by SUMO2 or SUMO3 by Evdokimov, Evgenij, Sharma, Prashant, Lockett, Stephen J, Lualdi, Margaret, Kuehn, Michael R

“…Conjugation of the small ubiquitin-like modifier (SUMO) to target proteins regulates numerous biological processes and has been implicated in tumorigenesis and…”
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Identification of Developmentally Expressed Proteins That Functionally Interact with Nedd4 Ubiquitin Ligase by Murillas, Rodolfo, Simms, Kimberly S., Hatakeyama, Shigetsugu, Weissman, Allan M., Kuehn, Michael R.

“…Nedd4 is a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins. The molecular basis for the…”
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Inhibiting Smad2/3 signaling in pluripotent mouse embryonic stem cells enhances endoderm formation by increasing transcriptional priming of lineage‐specifying target genes by Dahle, Øyvind, Kuehn, Michael R.

“…Background: Pluripotent embryonic stem cells (ESCs) offer great potential for regenerative medicine. However, efficient in vitro generation of specific desired…”
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N4BP1 is a newly identified nucleolar protein that undergoes SUMO-regulated polyubiquitylation and proteasomal turnover at promyelocytic leukemia nuclear bodies by Sharma, Prashant, Murillas, Rodolfo, Zhang, Huafeng, Kuehn, Michael R

“…A number of proteins can be conjugated with both ubiquitin and the small ubiquitin-related modifier (SUMO), with crosstalk between these two post-translational…”
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Sonic hedgehog signaling directly targets Hyaluronic Acid Synthase 2, an essential regulator of phalangeal joint patterning by Liu, Jiang, Li, Qiang, Kuehn, Michael R., Litingtung, Ying, Vokes, Steven A., Chiang, Chin

“…Sonic hedgehog (Shh) signal, mediated by the Gli family of transcription factors, plays an essential role in the growth and patterning of the limb. Through…”
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Polycomb Determines Responses to Smad2/3 Signaling in Embryonic Stem Cell Differentiation and in Reprogramming by Dahle, Øyvind, Kuehn, Michael R.

“…Integration of extrinsic signals, epigenetic regulators, and intrinsic transcription factors establishes pluripotent stem cell identity. Interplay between…”
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Loss of function of mouse Pax‐Interacting Protein 1‐associated glutamate rich protein 1a (Pagr1a) leads to reduced Bmp2 expression and defects in chorion and amnion development by Kumar, Amit, Lualdi, Margaret, Loncarek, Jadranka, Cho, Young‐Wook, Lee, Ji‐Eun, Ge, Kai, Kuehn, Michael R.

“…Background: Human PAX‐Interacting Protein 1 (PAXIP1)‐associated glutamate rich protein 1 (PAGR1, also known as PA1) originally was discovered as part of a…”
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Midline signaling regulates kidney positioning but not nephrogenesis through Shh by Tripathi, Piyush, Guo, Qiusha, Wang, Yinqiu, Coussens, Matthew, Liapis, Helen, Jain, Sanjay, Kuehn, Michael R., Capecchi, Mario R., Chen, Feng

“…The role of axial structures, especially the notochord, in metanephric kidney development has not been directly examined. Here, we showed that disruption of…”
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