Genomic analysis and in vivo efficacy of Pediococcus acidilactici as a potential probiotic to prevent hyperglycemia, hypercholesterolemia and gastrointestinal infections

Lactic acid bacteria are the well acknowledged probiotics that can cure a variety of diseases. In this study, we observed the in vivo potentials of Pediococcus to treat hyperglycemia, hypercholesterolemia and gastrointestinal infections. A total of 77 Lactobacillus were isolated from the milk of 10...

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Published in:	Scientific reports Vol. 12; no. 1; p. 20429
Main Authors:	Al-Emran, Hassan M., Moon, Jannatul Ferdouse, Miah, Md. Liton, Meghla, Nigar Sultana, Reuben, Rine Christopher, Uddin, Mohammad Jashim, Ibnat, Habiba, Sarkar, Shovon Lal, Roy, Pravas Chandra, Rahman, M. Shaminur, Alam, A. S. M. Rubayet Ul, Islam, Ovinu Kibria, Jahid, Iqbal Kabir
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 28-11-2022 Nature Publishing Group Nature Portfolio
Subjects:	631/250 631/326/41 Animals Bacteria Blood Carbohydrates Cattle Communicable Diseases Cow's milk Diabetes mellitus DNA sequencing Drug resistance E coli Electron microscopy Escherichia coli Female Gastrointestinal Diseases Genomic analysis Genomics Goats Humanities and Social Sciences Humans Hypercholesterolemia Hyperglycemia Hyperglycemia - prevention & control Immunomodulation Infections Lactic acid Lactic acid bacteria Metformin Mice multidisciplinary Nucleotide sequence Pediococcus Pediococcus - genetics Pediococcus acidilactici Pediococcus acidilactici - genetics Probiotics Probiotics - pharmacology Probiotics - therapeutic use RNA, Ribosomal, 16S - genetics rRNA 16S Science Science (multidisciplinary) Sequence analysis Strains (organisms) α-Amylase α-Glucosidase
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Summary:	Lactic acid bacteria are the well acknowledged probiotics that can cure a variety of diseases. In this study, we observed the in vivo potentials of Pediococcus to treat hyperglycemia, hypercholesterolemia and gastrointestinal infections. A total of 77 Lactobacillus were isolated from the milk of 10 cows and 10 goats, four of those strains inhibited both carbohydrates-hydrolyzing enzymes, α-glucosidase, and α-amylase. They all showed antagonistic effects on pathogenic E. coli and S. Typhimurium which were confirmed by performing pathogen challenge test and visualizing on Electron microscopy. 16S rRNA gene sequence identified that all four strains belong to Pediococcu s genus which were further distinguished as Pediococcus acidilactici by phe S gene sequence. Whole genome sequence analysis revealed their non-pathogenic properties for human and the presence of probiotic genes responsible for stress resistance, immunomodulation, adhesion, metal and drug resistance. In vivo trial with diabetes-induced mice ascertained that all Pediococcus acidilactici had significant potentials to reduce elevated glucose and low-density lipoprotein level in blood. Interestingly, two out of four strains were significantly more effective (p < 0.0001 each) than metformin in reducing the blood glucose level. This in vivo study demonstrated that Pediococcus acidilactici might be a promising probiotic to prevent hyperglycemia, hypercholesterolemia and gastrointestinal infections.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-022-24791-5