Search Results - "Mezgueldi, M"

Role of tropomyosin in the regulation of contraction in smooth muscle by Marston, Steve, El-Mezgueldi, M

“…Smooth muscle contraction is due to the interaction ofmyosin filaments with thin filaments. Thin filaments are composed of actin, tropomyosin, caldesmon and…”
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Mutations in tropomyosin that cause DCM, affect cooperativity of cardiac muscle thin filaments by Marston, S, Mirza, M, Robinson, P, Kremneva, E, Levitsky, D, Redwood, C, EL-Mezgueldi, M

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Mapping of the functional domains in the amino-terminal region of calponin by MEZGUELDI, M, FATTOUM, A, DERANCOURT, J, KASSAB, R

“…Three chymotryptic fragments accounting for almost the entire amino acid sequence of gizzard calponin (Takahashi, K., and Nadal-Ginard, B. (1991) J. Biol…”
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3-D image reconstruction of reconstituted smooth muscle thin filaments containing calponin: Visualization of interactions between F-actin and Calponin by Hodgkinson, J.L., EL-Mezgueldi, M., Craig, R., Vibert, P., Marston, S.B., Lehman, W.

“…Calponin is a putative thin filament regulatory protein of smooth muscle that inhibits actomyosin ATPase in vitro. We have used electron microscopy and…”
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Precise identification of the regulatory F-actin- and calmodulin-binding sequences in the 10-kDa carboxyl-terminal domain of caldesmon by Mezgueldi, M, Derancourt, J, Calas, B, Kassab, R, Fattoum, A

“…The precise location of the regulatory F-actin- and calmodulin-binding sites in the COOH-terminal sequence Trp659-Pro756 of gizzard caldesmon was investigated…”
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Structural interactions between actin, tropomyosin, caldesmon and calcium binding protein and the regulation of smooth muscle thin filaments by MARSTON, S., BURTON, D., COPELAND, O., FRASER, I., GAO, Y., HODGKINSON, J., HUBER, P., LEVINE, B., EL-MEZGUELDI, M., NOTARIANNI, G.

“…ABSTRACT The basic structure and functional properties of smooth muscle thin filaments were established about 10 years ago. Since then we and others have been…”
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Calponin by El-Mezgueldi, Mohammed

“…Calponin is a troponin-T like protein purified from chicken gizzard smooth muscle. It binds to actin, myosin, Ca 2+-binding proteins and tropomyosin and…”
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Characterization of the regulatory domain of gizzard calponin. Interactions of the 145-163 region with F-actin, calcium-binding proteins, and tropomyosin by Mezgueldi, M, Mendre, C, Calas, B, Kassab, R, Fattoum, A

“…Earlier, we proposed that the interaction of gizzard calponin with F-actin, promoting the inhibition of the actomyosin ATPase activity, involves the…”
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Cooperative inhibition of actin filaments in the absence of tropomyosin by Ansari, Saira, El-Mezgueldi, Mohammed, Marston, Steven

“…We measured the inhibition of actin activated myosin subfragment-1 MgATPase activity in a solution containing no added KCl (5 mM PIPES.K2 (pH 7.1), 2.5 mM…”
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Multiple-sited interaction of caldesmon with Ca(2+)-calmodulin by Huber, P A, El-Mezgueldi, M, Grabarek, Z, Slatter, D A, Levine, B A, Marston, S B

“…The binding of Ca(2+)- and Ba(2+)-calmodulin to caldesmon and its functional consequence was investigated with three different calmodulin mutants. Two…”
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The Effects of Smooth Muscle Calponin on the Strong and Weak Myosin Binding Sites of F-actin by EL-Mezgueldi, Mohammed, Marston, Steven B.

“…We have investigated the mechanism of inhibition of the actomyosin MgATPase by the smooth muscle protein calponin. We have shown previously the specific…”
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The Interface between Caldesmon Domain 4b and Subdomain 1 of Actin Studied by Nuclear Magnetic Resonance Spectroscopy by Gao, Yuan, Patchell, Valerie B, Huber, Pia A. J, Copeland, Oneal, El-Mezgueldi, Mohammed, Fattoum, A, Calas, Bernard, Thorsted, Peter B, Marston, Steven B, Levine, Barry A

“…The ability of caldesmon to inhibit actomyosin ATPase activity involves the interaction of three nonsequential segments of caldesmon domain 4 (amino acids…”
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Expressing Functional Domains of Mouse Calponin:  Involvement of the Region Around Alanine 145 in the Actomyosin ATPase Inhibitory Activity of Calponin by EL-Mezgueldi, Mohammed, Strasser, Peter, Fattoum, Abdelatif, Gimona, Mario

“…Previously, we attributed the binding of F-actin to the 38-residue stretch of gizzard calponin encompassing the sequence A145−Y182 and postulated the…”
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Structure−Activity Studies of the Regulatory Interaction of the 10 Kilodalton C-Terminal Fragment of Caldesmon with Actin and the Effect of Mutation of Caldesmon Residues 691−696 by Huber, Pia A. J, Gao, Yuan, Fraser, Iain D. C, Copeland, O'neal, EL-Mezgueldi, Mohammed, Slatter, David A, Keane, Noeleen E, Marston, Steven B, Levine, Barry A

“…We have used isotope-edited nuclear magnetic resonance spectroscopy, binding studies, and ATPase activity assays to investigate the interaction with F-actin of…”
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Characterization of the functional properties of smooth muscle caldesmon domain 4a: evidence for an independent inhibitory actin-tropomyosin binding domain by El-Mezgueldi, M, Copeland, O, Fraser, I D, Marston, S B, Huber, P A

“…Recent analysis has shown the presence of three sequences in the C-terminal 170 amino acids of human caldesmon (domain 4) which are involved in actin binding…”
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Disruption of the actin cytoskeleton in living nonmuscle cells by microinjection of antibodies to domain-3 of caldesmon by Lamb, N J, Fernandez, A, Mezgueldi, M, Labbé, J P, Kassab, R, Fattoum, A

“…Two classes of affinity-purified polyclonal antibodies directed against the four different domains of gizzard caldesmon were prepared and their specificity was…”
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Characterisation of the effects of mutation of the caldesmon sequence 691glu‐trp‐leu‐thr‐lys‐thr696 to pro‐gly‐his‐tyr‐asn‐asn on caldesmon‐calmodulin interaction by Huber, Pia A.J, Levine, Barry A, Copeland, O'neal, Marston, Steven B, EL-Mezgueldi, Mohammed

“…We have investigated the functional properties of a mutant (Cg1) derived from the C‐terminal 99 amino acids of chicken caldesmon, 658–756 (658C) where the…”
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Phosphorylation of the minimal inhibitory region at the C-terminus of caldesmon alters its structural and actin binding properties by Patchell, Valerie B., Vorotnikov, Alexander V., Gao, Yuan, Low, Douglas G., Evans, James S., Fattoum, Abdellatif, El-Mezgueldi, Mohammed, Marston, Steven B., Levine, Barry A.

“…Caldesmon is an inhibitory protein believed to be involved in the regulation of thin filament activity in smooth muscles and is a major cytoplasmic substrate…”
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