Increasing the sensitivity of endocervical curettings by performing ThinPrep® Pap on transport container fluid: is diagnostic material going down the drain?
Objective The sensitivity of endocervical curettage (ECC) can be suboptimal because of limited epithelial tissue. The false‐negative rate for ECC in patients with cervical intraepithelial neoplasia involving the endocervical canal has been reported to be 45%. ECC samples are transported to pathology...
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| Published in: | Cytopathology (Oxford) Vol. 26; no. 6; pp. 368 - 372 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
Blackwell Publishing Ltd
01-12-2015
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Objective
The sensitivity of endocervical curettage (ECC) can be suboptimal because of limited epithelial tissue. The false‐negative rate for ECC in patients with cervical intraepithelial neoplasia involving the endocervical canal has been reported to be 45%. ECC samples are transported to pathology in formalin‐ or saline‐filled containers; this fluid is discarded after the specimen has been submitted. We evaluated the utility of performing liquid‐based cytological preparations from ECC transport container fluid as a way to increase the sensitivity of ECC specimens.
Methods
Consecutive ECC specimens received at one of the two participating institutions were selected prospectively. A surgical pathology mesh bag was placed over a ThinPrep® CytoLyt® solution container, and the specimen was filtered through the bag, collecting the transport fluid in the container. The CytoLyt® was processed to obtain a container fluid ThinPrep® (CF‐TP) liquid‐based Papanicolaou (Pap) slide. The CF‐TP slides were reviewed and the findings were compared with those from the ECC and follow‐up specimens.
Results
The cohort included 53 patients. Discrepancies between CF‐TP and ECC were seen in 14 of the 53 patients (26%); a more significant lesion was identified in CF‐TP relative to ECC in 13 of these cases. CF‐TP diagnosis was confirmed in eight of 11 cases with histological follow‐up. A positive CF‐TP result was confirmed by histology in six of nine cases with negative ECC.
Conclusions
Combining the pathological evaluation of ECC specimens with liquid‐based cytology performed on the transport container fluid can increase the diagnostic sensitivity of the ECC procedure for the detection of cervical lesions. |
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| Bibliography: | ark:/67375/WNG-G0PGX2S0-3 istex:861621B14371F926D8244559BF8BF33FD6163B11 ArticleID:CYT12177 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0956-5507 1365-2303 |
| DOI: | 10.1111/cyt.12177 |