Search Results - "Metzger, Louis E"

Gene Therapy-Mediated Partial Reprogramming Extends Lifespan and Reverses Age-Related Changes in Aged Mice by Macip, Carolina Cano, Hasan, Rokib, Hoznek, Victoria, Kim, Jihyun, Lu, Yuancheng Ryan, Metzger, 4th, Louis E, Sethna, Saumil, Davidsohn, Noah

“…Aging is a complex progression of changes best characterized as the chronic dysregulation of cellular processes leading to deteriorated tissue and organ…”
Get more information

Targeted lipopolysaccharide biosynthetic intermediate analysis with normal-phase liquid chromatography mass spectrometry by Sawyer, William S, Wang, Lisha, Uehara, Tsuyoshi, Tamrakar, Pramila, Prathapam, Ramadevi, Mostafavi, Mina, Metzger, 4th, Louis E, Feng, Brian, Baxter Rath, Christopher M

“…Lipopolysacharride (LPS) forms the outer leaflet of the outer membrane in Gram-negative bacteria and contributes to the permeability barrier and immune…”
Get full text

Toxic Accumulation of LPS Pathway Intermediates Underlies the Requirement of LpxH for Growth of Acinetobacter baumannii ATCC 19606 by Richie, Daryl L, Takeoka, Kenneth T, Bojkovic, Jade, Metzger, 4th, Louis E, Rath, Christopher M, Sawyer, William S, Wei, Jun-Rong, Dean, Charles R

“…The lipid A moiety of lipopolysaccharide (LPS) is the main constituent of the outer leaflet of the Gram-negative bacterial outer membrane (OM) and is essential…”
Get full text

Structure-guided enzymology of the lipid A acyltransferase LpxM reveals a dual activity mechanism by Dovala, Dustin, Rath, Christopher M., Hu, Qijun, Sawyer, William S., Shia, Steven, Elling, Robert A., Knapp, Mark S., Metzger, Louis E.

“…Gram-negative bacteria possess a characteristic outer membrane, of which the lipid A constituent elicits a strong host immune response through the Toll-like…”
Get full text

Mode of Action of the Monobactam LYS228 and Mechanisms Decreasing In Vitro Susceptibility in Escherichia coli and Klebsiella pneumoniae by Dean, Charles R, Barkan, David T, Bermingham, Alun, Blais, Johanne, Casey, Fergal, Casarez, Anthony, Colvin, Richard, Fuller, John, Jones, Adriana K, Li, Cindy, Lopez, Sara, Metzger, 4th, Louis E, Mostafavi, Mina, Prathapam, Ramadevi, Rasper, Dita, Reck, Folkert, Ruzin, Alexey, Shaul, Jacob, Shen, Xiaoyu, Simmons, Robert L, Skewes-Cox, Peter, Takeoka, Kenneth T, Tamrakar, Pramila, Uehara, Tsuyoshi, Wei, Jun-Rong

“…The monobactam scaffold is attractive for the development of new agents to treat infections caused by drug-resistant Gram-negative bacteria because it is…”
Get full text

Purification and Characterization of the Lipid A Disaccharide Synthase (LpxB) from Escherichia coli, a Peripheral Membrane Protein by Metzger, Louis E, Raetz, Christian R. H

“…Escherichia coli LpxB, an inverting glycosyl transferase of the GT-B superfamily and a member of CAZy database family 19, catalyzes the fifth step of lipid A…”
Get full text

Different Poses for Ligand and Chaperone in Inhibitor-Bound Hsp90 and GRP94: Implications for Paralog-Specific Drug Design by Immormino, Robert M., Metzger, Louis E., Reardon, Patrick N., Dollins, D. Eric, Blagg, Brian S.J., Gewirth, Daniel T.

“…Hsp90 chaperones contain an N-terminal ATP binding site that has been effectively targeted by competitive inhibitors. Despite the myriad of inhibitors, none to…”
Get full text

Rapid analysis of protein expression and solubility with the SpyTag–SpyCatcher system by Dovala, Dustin, Sawyer, William S., Rath, Christopher M., Metzger, Louis E.

“…•Fluorescent SpyCatcher can be used to probe Western blots akin to antibodies.•Fluorescent SpyCatcher enables Western-like detection directly in SDS…”
Get full text

LpxI structures reveal how a lipid A precursor is synthesized by Metzger, Louis E, Lee, John K, Finer-Moore, Janet S, Raetz, Christian R H, Stroud, Robert M

“…In Gram-negative bacterial outer membranes, lipid A serves as the anchor for lipopolysaccharide. Structural and biochemical data reveal that LpxI, an enzyme…”
Get full text

An Alternative Route for UDP-Diacylglucosamine Hydrolysis in Bacterial Lipid A Biosynthesis by Metzger, Louis E, Raetz, Christian R. H

“…The outer leaflet of the outer membranes of Gram-negative bacteria is composed primarily of lipid A, the hydrophobic anchor of lipopolysaccharide. Like…”
Get full text

LpxK Is Essential for Growth of Acinetobacter baumannii ATCC 19606: Relationship to Toxic Accumulation of Lipid A Pathway Intermediates by Wei, Jun-Rong, Richie, Daryl L, Mostafavi, Mina, Metzger, 4th, Louis E, Rath, Christopher M, Sawyer, William S, Takeoka, Kenneth T, Dean, Charles R

“…ATCC 19606 can grow without lipid A, the major component of lipooligosaccharide. However, we previously reported that depletion of LpxH (the fourth enzyme in…”
Get full text

17-β-Hydroxysteroid dehydrogenase type 1: computational design of active site inhibitors targeted to the Rossmann fold by Brown, William M, Metzger, Louis E, Barlow, Jeremy P, Hunsaker, Lucy A, Deck, Lorraine M, Royer, Robert E, Vander Jagt, David L

“…17-β-Hydroxysteroid dehydrogenase type 1 (17βHSD1), also called estradiol dehydrogenase, catalyzes the NADPH-dependent reduction of the weak estrogen, estrone,…”
Get full text

LpxI: A Novel Phosphosaccharolipid Hydrolase by Metzger, Louis E., Lee, John K., Finer-Moore, Janet S., Raetz, Christian R.H., Stroud, Robert M.

Get full text

Enzymatic and structural studies of UDP‐2,3‐diacylglucosamine hydrolysis in lipid A biosynthesis by Young, Hayley E., Metzger, Louis E., Lee, John K., Stroud, Robert M., Zhou, Pei, Raetz, Christian R. H.

“…Abstract only The outer‐leaflet of the outer membrane of Gram‐negative bacteria is composed of lipopolysaccharide (LPS), which is attached to the membrane via…”
Get full text