Search Results - "Methods in Kidney Cell Biology Part A"

Application of physiological shear stress to renal tubular epithelial cells by Ferrell, Nicholas, Sandoval, Ruben M, Molitoris, Bruce A, Brakeman, Paul, Roy, Shuvo, Fissell, William H

“…Renal tubular epithelial cells are consistently exposed to flow of glomerular filtrate that creates fluid shear stress at the apical cell surface. This…”
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Differentiation of human kidney organoids from pluripotent stem cells by Cruz, Nelly M, Freedman, Benjamin S

“…It is now possible to direct the differentiation of human pluripotent stem cells into three-dimensional nephron-like structures called kidney organoids…”
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In vitro cyst formation of ADPKD cells by Sharma, Madhulika, Reif, Gail A, Wallace, Darren P

“…Autosomal dominant polycystic kidney disease (ADPKD) is a common genetic disorder characterized by the relentless growth of numerous fluid-filled cysts in the…”
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Investigation of epigenetics in kidney cell biology by Li, Linda Xiaoyan, Agborbesong, Ewud, Zhang, Lu, Li, Xiaogang

“…Epigenetics is the study of heritable changes in DNA or its associated proteins except mutations in gene sequence. Epigenetic regulation plays fundamental…”
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Analysis of primary cilia in renal tissue and cells by Silva, Luciane M, Wang, Wei, Allard, Bailey A, Pottorf, Tana S, Jacobs, Damon T, Tran, Pamela V

“…Primary cilia are singular, sensory organelles that extend from the plasma membrane of most quiescent mammalian cells. These slender, microtubule-based…”
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ADPKD cell proliferation and Cl--dependent fluid secretion by Reif, Gail A, Wallace, Darren P

“…Autosomal dominant polycystic kidney disease (ADPKD) is a common genetic disorder characterized by bilateral fluid-filled cysts, renal inflammation and…”
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Generation of primary cells from ADPKD and normal human kidneys by Wallace, Darren P, Reif, Gail A

“…Autosomal dominant polycystic kidney (ADPKD) is a common genetic disorder characterized by the presence of numerous fluid-filled cysts that lead to a…”
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Visualizing gene expression during zebrafish pronephros development and regeneration by Marra, Amanda N, Chambers, Brooke E, Chambers, Joseph M, Drummond, Bridgette E, Adeeb, Basma D, Wesselman, Hannah M, Morales, Elvin E, Handa, Nicole, Pettini, Tom, Ronshaugen, Matthew, Wingert, Rebecca A

“…The vertebrate kidney is comprised of functional units known as nephrons. Defects in nephron development or activity are a common feature of kidney disease…”
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Ex vivo kidney slice preparations as a model system to study signaling cascades in kidney epithelial cells by Saitta, Biagio, Jalili, Michael F, Zohoorkari, Hamidreza, Rao, Renee, Hallows, Kenneth R, Baty, Catherine J, Pastor-Soler, Nuria M

“…Several model systems have been used to study signaling cascades in kidney epithelial cells, including kidney histology after systemic treatments, ex vivo…”
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Engineering kidney tissues for polycystic kidney disease modeling and drug discovery by Brizi, Valerio, Benedetti, Valentina, Lavecchia, Angelo Michele, Xinaris, Christodoulos

“…Autosomal dominant polycystic kidney disease (ADPKD) is the most prevalent, potentially lethal monogenic human disorder. There is currently no cure for ADPKD…”
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Metanephric organ culture by Maser, Robin L, Magenheimer, Brenda S, Calvet, James P

“…Metanephric organ culture, or ex vivo embryonic kidney culture, was developed in the mid-twentieth century as a means to understand the development of the…”
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Manipulation of renal gene expression using oligonucleotides by Lakhia, Ronak, Mishra, Abheepsa, Patel, Vishal

“…Oligonucleotides are small molecules 8-50 nucleotides in length that bind via Watson-Crick base pairing to enhance or repress the expression of target RNA. The…”
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Novel fluorescence techniques to quantitate renal cell biology by Shroff, Urvi Nikhil, Schiessl, Ina Maria, Gyarmati, Georgina, Riquier-Brison, Anne, Peti-Peterdi, Janos

“…Fluorescence microscopy techniques are powerful tools to study tissue dynamics, cellular function and biology both in vivo and in vitro. These tools allow for…”
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Studying Na+ and K+ channels in aldosterone-sensitive distal nephrons by Teulon, Jacques, Wang, Wen-Hui

“…Aldosterone-sensitive distal nephron (ASDN) including the distal convoluted tubule (DCT), connecting tubule (CNT) and collecting duct (CD) plays an important…”
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Measurement of cytoplasmic and cilioplasmic calcium in a single living cell by Sherpa, Rinzhin T, Pala, Rajasekharreddy, Mohieldin, Ashraf M, Nauli, Surya M

“…Cellular signaling represents an evolution of biological systems to sense external stimuli and communicate extracellular microenvironment to the intracellular…”
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Quantifying autophagic flux in kidney tissue using structured illumination microscopy by Taguchi, Kensei, Elias, Bertha C, Qian, Subo, Brooks, Craig R

“…Kidney disease is estimated to affect 15% of the world's population. Autophagy is a key homeostatic pathway in eukaryotic cells, which has been linked to…”
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Urinary extracellular vesicles as a source of biomarkers reflecting renal cellular biology in human disease by Braun, Fabian, Müller, Roman-Ulrich

“…For more than a decade, extracellular vesicles (EVs) have been the focus of extensive research efforts attempting to uncover their biological function in…”
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Drosophila melanogaster and its nephrocytes: A versatile model for glomerular research by Odenthal, Johanna, Brinkkoetter, Paul Thomas

“…Glomerular disorders are a predominant cause of chronic kidney diseases and end-stage renal failure. Especially podocytes, epithelial cells which represent the…”
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In vivo analysis of renal epithelial cells in zebrafish by Li, Yuanyuan, Xu, Wenyan, Jerman, Stephanie, Sun, Zhaoxia

“…The zebrafish kidney has been used effectively for studying kidney development, repair and disease. New gene editing capability makes it a more versatile in…”
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Single glomerular proteomics: A novel tool for translational glomerular cell biology by Rinschen, Markus M

“…The glomerulus harbors the renal filtration barrier and needs to be precisely maintained. In this chapter, the concept of single glomerular proteomics is…”
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