Search Results - "Mestrom, Luuk"

Thermodynamically and Kinetically Controlled Reactions in Biocatalysis – from Concepts to Perspectives by Marsden, Stefan R., Mestrom, Luuk, McMillan, Duncan G. G., Hanefeld, Ulf

“…The enzymatic synthesis of esters and peptides is unfavoured in aqueous solvent systems due to competing hydrolysis. This can be overcome by using energy rich…”
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Leloir Glycosyltransferases in Applied Biocatalysis: A Multidisciplinary Approach by Mestrom, Luuk, Przypis, Marta, Kowalczykiewicz, Daria, Pollender, André, Kumpf, Antje, Marsden, Stefan R, Bento, Isabel, Jarzębski, Andrzej B, Szymańska, Katarzyna, Chruściel, Arkadiusz, Tischler, Dirk, Schoevaart, Rob, Hanefeld, Ulf, Hagedoorn, Peter-Leon

“…Enzymes are nature's catalyst of choice for the highly selective and efficient coupling of carbohydrates. Enzymatic sugar coupling is a competitive technology…”
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Amino Aldehydes Revisited by Mestrom, Luuk, Bracco, Paula, Hanefeld, Ulf

“…The enzymatic oxidation of amino alcohols was studied to address the long‐standing problem of product stability. Amino aldehydes, highly sought and unstable…”
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Beyond the Chemical Step: The Role of Substrate Access in Acyltransferase from Mycobacterium smegmatis by Carvalho, Henrique F., Mestrom, Luuk, Hanefeld, Ulf, Pleiss, Jürgen

“…Acyltransferase from Mycobacterium smegmatis is a versatile enzyme, which catalyzes the transesterification of esters in aqueous media due to a kinetic…”
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Enzyme‐Catalyzed Synthesis of Esters in Water by Mestrom, Luuk, Claessen, Jord G. R., Hanefeld, Ulf

“…MsAcT catalyzes the esterification of primary alcohols in water. When utilizing acid and alcohol as starting materials low yields dictated by thermodynamics…”
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Engineering of continuous bienzymatic cascade process using monolithic microreactors – In flow synthesis of trehalose by Kowalczykiewicz, Daria, Przypis, Marta, Mestrom, Luuk, Kumpf, Antje, Tischler, Dirk, Hagedoorn, Peter-Leon, Hanefeld, Ulf, Jarzębski, Andrzej, Szymańska, Katarzyna

“…[Display omitted] •A continuous bi-enzymatic trehalose synthesis from basic sugars is developed.•UDP-glucose pyrophosphorylase and trehalose…”
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Ester Synthesis in Water: Mycobacterium smegmatis Acyl Transferase for Kinetic Resolutions by de Leeuw, Nicolas, Torrelo, Guzman, Bisterfeld, Carolin, Resch, Verena, Mestrom, Luuk, Straulino, Emanuele, van der Weel, Laura, Hanefeld, Ulf

“…The acyl transferase from Mycobacterium smegmatis (MsAcT) catalyses transesterification reactions in aqueous media because of its hydrophobic active site…”
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Artificial Fusion of mCherry Enhances Trehalose Transferase Solubility and Stability by Mestrom, Luuk, Marsden, Stefan R, Dieters, Marit, Achterberg, Puck, Stolk, Lysanne, Bento, Isabel, Hanefeld, Ulf, Hagedoorn, Peter-Leon

“…LeLoir glycosyltransferases are important biocatalysts for the production of glycosidic bonds in natural products, chiral building blocks, and pharmaceuticals…”
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Substrate Induced Movement of the Metal Cofactor between Active and Resting State by Marsden, Stefan R., Wijma, Hein J., Mohr, Michael K. F., Justo, Inês, Hagedoorn, Peter‐Leon, Laustsen, Jesper, Jeffries, Cy M., Svergun, Dmitri, Mestrom, Luuk, McMillan, Duncan G. G., Bento, Isabel, Hanefeld, Ulf

“…Regulation of enzyme activity is vital for living organisms. In metalloenzymes, far‐reaching rearrangements of the protein scaffold are generally required to…”
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Cover Feature: Thermodynamically and Kinetically Controlled Reactions in Biocatalysis – from Concepts to Perspectives (ChemCatChem 2/2020) by Marsden, Stefan R., Mestrom, Luuk, McMillan, Duncan G. G., Hanefeld, Ulf

“…The Cover Feature shows the enzyme‐catalyzed epimerisation of a kinetic product into the thermodynamic product, where the moving gears depict the power of an…”
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A second shell residue modulates a conserved ATP-binding site with radically different affinities for ATP by Krah, Alexander, van der Hoeven, Bas, Mestrom, Luuk, Tonin, Fabio, Knobel, Kirsten C.C., Bond, Peter J., McMillan, Duncan G.G.

“…Prediction of ligand binding and design of new function in enzymes is a time-consuming and expensive process. Crystallography gives the impression that…”
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CH‐π Interactions Promote the Conversion of Hydroxypyruvate in a Class II Pyruvate Aldolase by Marsden, Stefan R., Mestrom, Luuk, Bento, Isabel, Hagedoorn, Peter‐Leon, McMillan, Duncan G. G., Hanefeld, Ulf

“…The class II hydroxy ketoacid aldolase A5VH82 from Sphingomonas wittichii RW1 (SwHKA) accepts hydroxypyruvate as nucleophilic donor substrate, giving access to…”
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Correction for Mestrom et al., "Artificial Fusion of mCherry Enhances Trehalose Transferase Solubility and Stability" by Mestrom, Luuk, Marsden, Stefan R, Dieters, Marit, Achterberg, Puck, Stolk, Lysanne, Bento, Isabel, Hanefeld, Ulf, Hagedoorn, Peter-Leon

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Thermodynamics Determine the Diastereochemical Outcome of Catalytic Reactions by Marsden, Stefan R., Mestrom, Luuk, Wijma, Hein J., Noordam, Sander J., McMillan, Duncan G. G., Hanefeld, Ulf

“…Diastereomers are characterised by an intrinsic energy difference, and thermodynamics dictate their distribution within a dynamic equilibrium. The…”
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Comparison of Enzymes Immobilised on Immobeads and Inclusion Bodies: A Case Study of a Trehalose Transferase by Mestrom, Luuk, Marsden, Stefan R., McMillan, Duncan G. G., Schoevaart, Rob, Hagedoorn, Peter‐Leon, Hanefeld, Ulf

“…In this case study, we compare the performance of an enzyme immobilised using two different methods: i) as carrier‐free catalytically active inclusion bodies…”
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Anomeric Selectivity of Trehalose Transferase with Rare l‑Sugars by Mestrom, Luuk, Marsden, Stefan R, van der Eijk, Hessel, Laustsen, Jesper U, Jeffries, Cy M, Svergun, Dmitri I, Hagedoorn, Peter-Leon, Bento, Isabel, Hanefeld, Ulf

“…Retaining LeLoir glycosyltransferases catalyze the formation of glycosidic bonds between nucleotide sugar donors and carbohydrate acceptors. The anomeric…”
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Substrate Induced Movement of the Metal Cofactor between Active and Resting State by Marsden, Stefan R., Wijma, Hein J., Mohr, Michael K. F., Justo, Inês, Hagedoorn, Peter‐Leon, Laustsen, Jesper, Jeffries, Cy M., Svergun, Dmitri, Mestrom, Luuk, McMillan, Duncan G. G., Bento, Isabel, Hanefeld, Ulf

“…Regulation of enzyme activity is vital for living organisms. In metalloenzymes, far‐reaching rearrangements of the protein scaffold are generally required to…”
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