Laser-fabricated cell patterning stencil for single cell analysis

Laser-fabricated cell patterning stencil for single cell analysis

Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning methods have been demonstrated, many of these methods subject cells to high stress environments...

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Bibliographic Details
Published in:BMC biotechnology Vol. 17; no. 1; p. 89
Main Authors: Messner, Jacob J, Glenn, Honor L, Meldrum, Deirdre R
Format: Journal Article
Language:English
Published: England BioMed Central Ltd 19-12-2017
BioMed Central
BMC
Subjects:
Adhesion
Analysis
Cell culture
Cell migration
Cell patterning
Cells (Biology)
Methodology
Microwell
Single-cell
Tissue engineering
Cell culture
Cell patterning
Adhesion
Single-cell
Microwell
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Description
Summary:Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning methods have been demonstrated, many of these methods subject cells to high stress environments, discriminate against certain phenotypes, or are a challenge to implement. Here, we demonstrate a rapid, simple, indiscriminate, and minimally perturbing cell patterning method using a laser fabricated polymer stencil. The stencil fabrication process requires no stencil-substrate alignment, and is readily adaptable to various substrate geometries and experiments.
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ISSN:1472-6750
1472-6750
DOI:10.1186/s12896-017-0408-8